3300000236_6 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000006)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	T2PKS	65,721	130,885

c00012_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000054)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS-like,betalactone	1	22,524

c00031_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000003)

Region	Type	From	To	Most similar known cluster		Similarity
Region 31.1	T1PKS,NRPS	1	48,728
Region 31.2	terpene	108,786	129,838

c00037_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000008)

Region	Type	From	To	Most similar known cluster		Similarity
Region 37.1	NRPS,T1PKS,bacteriocin	1	58,262

c00048_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000020)

Region	Type	From	To	Most similar known cluster		Similarity
Region 48.1	NRPS,betalactone	49,034	84,133

Region	Type	From	To	Similarity
Region 3.1	T2PKS	65,721	130,885
Region 12.1	NRPS-like,betalactone	1	22,524
Region 31.1	T1PKS,NRPS	1	48,728
Region 31.2	terpene	108,786	129,838
Region 37.1	NRPS,T1PKS,bacteriocin	1	58,262
Region 48.1	NRPS,betalactone	49,034	84,133

No secondary metabolite regions were found in these records:: c00001_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000525); c00002_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000014); c00004_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000015); c00005_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000028); c00006_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000594); c00007_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000011); c00008_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000361); c00009_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000001); c00010_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000007); c00011_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000010); c00013_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000071); c00014_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000056); c00015_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1003821); c00016_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000185); c00017_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000076); c00018_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000171); c00019_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000033); c00020_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000017); c00021_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000025); c00022_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000260); c00023_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000342); c00024_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000021); c00025_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000004); c00026_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1003635); c00027_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000145); c00028_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000074); c00029_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1002220); c00030_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000437); c00032_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000005); c00033_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000230); c00034_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000383); c00035_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000027); c00036_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000401); c00038_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000002); c00039_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000094); c00040_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000567); c00041_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000081); c00042_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000065); c00043_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000330); c00044_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000592); c00045_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000024); c00046_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000016); c00047_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000034); c00049_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000009); c00050_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000012); c00051_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000723); c00052_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1004747); c00053_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000013); c00054_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000019); c00055_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000079); c00056_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000030); c00057_3300000.. (original name was: 3300000236.a:TB_FS08_3DRAFT_1000202)

Compact view

c00003_3300000.. - Region 1 - T2PKS

Download region GenBank file

Location: 65,721 - 130,885 nt. (total: 65,165 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

Type II PKS

Type II PKS gene cluster prediction details

Cluster section:: 65720 to 130885
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Product class(es):: anthracycline
aureolic acid
tetracycline

ctg3_68: CYC C7-C12 (Score: 184.4; E-value: 5.3e-57)
ctg3_69: KR (Score: 243.3; E-value: 7.6e-75)
ctg3_70: CYC C5-C14/C3-C16 (Score: 295.7; E-value: 6.9e-91)
ctg3_78: KS (Score: 406.9; E-value: 2.1e-124)

c00012_3300000.. - Region 1 - NRPS-like,betalactone

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Location: 1 - 22,524 nt. (total: 22,524 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 22524:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg12_1

Search NORINE for peptide: strict or relaxed

AMP-binding (0..376): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

c00031_3300000.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 1 - 48,728 nt. (total: 48,728 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 48728:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr - thr) + (X - mal - X - cys - gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg31_4

Search NORINE for peptide: strict or relaxed

AMP-binding (472..869): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1856..2253): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg31_5

Search NORINE for peptide: strict or relaxed

AMP-binding (533..930): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVemIGAVsK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 70% (weak)

PKS_AT (1657..1936): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 159.6
inactive: 115.4
Methoxymalonyl-CoA: 62.3
Methylmalonyl-CoA: 50.9
Propionyl-CoA: 37.7
Ethylmalonyl-CoA: 28.1
2-Methylbutyryl-CoA: 20.8
Benzoyl-CoA: 19.4
Isobutyryl-CoA: 16.7
CHC-CoA: 16.7
Acetyl-CoA: 15.9
trans-1,2-CPDA: 10.9
3-Methylbutyryl-CoA: 8.0
fatty_acid: 0.0

AMP-binding (2599..3006): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLwYLgGVcK
Nearest Stachelhaus code:: iva
Stachelhaus code match:: 70% (weak)

AMP-binding (4060..4347): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYdfSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 80% (moderate)

AMP-binding (5004..5399): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

c00031_3300000.. - Region 2 - terpene

Download region GenBank file

Location: 108,786 - 129,838 nt. (total: 21,053 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

c00037_3300000.. - Region 1 - NRPS,T1PKS,bacteriocin

Download region GenBank file

Location: 1 - 58,262 nt. (total: 58,262 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 58262:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (thr - val - val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg37_16

PKS_AT (533..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 158.7
inactive: 119.9
Methoxymalonyl-CoA: 52.8
Methylmalonyl-CoA: 37.5
Propionyl-CoA: 23.5
trans-1,2-CPDA: 18.1
Isobutyryl-CoA: 15.6
fatty_acid: 14.7
Acetyl-CoA: 14.5
Ethylmalonyl-CoA: 14.4
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg37_19

Search NORINE for peptide: strict or relaxed

AMP-binding (16..413): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DVWnMgLVnK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

AMP-binding (1475..1874): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAfWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

AMP-binding (2532..2931): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAfWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

c00048_3300000.. - Region 1 - NRPS,betalactone

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Location: 49,034 - 84,133 nt. (total: 35,100 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 49033 - 84133:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg48_65

Search NORINE for peptide: strict or relaxed

AMP-binding (17..428): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (1089..1515): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAfWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)