LOCUS       c00544_3300002..        4835 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  3300002858.a:JGI1201J43222_100623.
ACCESSION   c00544_3300002..
VERSION     c00544_3300002..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 5.1.0
            Run date     :: 2019-11-23 04:47:05
            Original ID  :: 3300002858.a:JGI1201J43222_100623
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4835
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4835
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[252:2253](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      253..2253
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..4835
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4835
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /tool="antismash"
     CDS             complement(3..251)
                     /locus_tag="ctg544_1"
                     /transl_table=11
                     /translation="MKNPLHYIQEYPQRTKQILGISNEQFQELLNQAEIHHNKIQAEIE
                     RKKIRINEKGGGRKPKLEVKEEICLCLFYLRQMPTFEV"
     CDS             253..2253
                     /NRPS_PKS="Domain: CAL_domain (51-371). E-value: 7.4e-112.
                     Score: 365.9. Matches aSDomain:
                     nrpspksdomains_ctg544_2_CAL_domain.1"
                     /NRPS_PKS="Domain: ACP (579-638). E-value: 1.5e-18. Score:
                     58.6. Matches aSDomain: nrpspksdomains_ctg544_2_ACP.1"
                     /NRPS_PKS="type: other"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     304.6; E-value: 1.7e-92)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg544_2"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-80, bitscore:
                     259.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.6e-16, bitscore:
                     48.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LVDGENSVLPFFWHQVNIPDRSNIENDLDENDLDENNLTREWREP
                     ITNSHTLAVFQYTSGSTGKPKGVMLTHGNLLHNSALIHQCFGHSKDSQGVIWLPPYHDM
                     GLIGGIIQPLYGGFPVTLMSPVDFLQKPYRWLQAISRYKATTSGGPNFAYDLCMHKITE
                     EQRATLDLSSWEVAFNGAEPIRAETLEQFVAVFEPCGFRKEAFYPCYGMAESTLIISGG
                     LKDSQPVYKTIQANRLEENRVVLTTKDNHKNLTLVSCGQTRMNQKITIADPEKLTSCSP
                     GQVGEIWVSGQSIAQGYWNCSEQTKLNFQAYLLDSGEGPFLRTGDLGFIEDGELYITGR
                     IKDLIIIRGRNHYPQDIEQTVQTSHPALKMGHGAAFSVEVEGNETLVITQEVKRSYLRK
                     LNINQVVTAICQAVAQEHEIQVSGILLLKTGSIPKTSSGKIQRYACRQGFMNNTLNVVS
                     DWTSSPRMKSEFRNLQAELNQIEQDLHTNRSPHQSLIKSDSQVNGQGNYGENIQTQRIS
                     VVKPPNSYFSQTEETQIQNISVTQIQEQNTCDNQEMLSSSHNSLEAIQNLLVVRIIDYL
                     KANATHLNVDDIDIQKPLTTYGLDSLALIELSAQLENWLGFGLSPTTLYEYPTIKTLAE
                     YLDSLQNQKLEAKVEELSEQQIDSVLQKLLD"
     aSDomain        406..1365
                     /aSDomain="CAL_domain"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg544_2_CAL_domain.1"
                     /evalue="7.40E-112"
                     /label="ctg544_2_CAL_domain.1"
                     /locus_tag="ctg544_2"
                     /protein_end="371"
                     /protein_start="51"
                     /score="365.9"
                     /specificity="Minowa: NH2"
                     /tool="antismash"
                     /translation="LAVFQYTSGSTGKPKGVMLTHGNLLHNSALIHQCFGHSKDSQGVI
                     WLPPYHDMGLIGGIIQPLYGGFPVTLMSPVDFLQKPYRWLQAISRYKATTSGGPNFAYD
                     LCMHKITEEQRATLDLSSWEVAFNGAEPIRAETLEQFVAVFEPCGFRKEAFYPCYGMAE
                     STLIISGGLKDSQPVYKTIQANRLEENRVVLTTKDNHKNLTLVSCGQTRMNQKITIADP
                     EKLTSCSPGQVGEIWVSGQSIAQGYWNCSEQTKLNFQAYLLDSGEGPFLRTGDLGFIED
                     GELYITGRIKDLIIIRGRNHYPQDIEQTVQTSHPALKMGH"
     aSModule        406..2166
                     /domains="nrpspksdomains_ctg544_2_CAL_domain.1"
                     /domains="nrpspksdomains_ctg544_2_ACP.1"
                     /incomplete
                     /locus_tags="ctg544_2"
                     /starter_module
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       412..468
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg544_2_0001"
                     /evalue="8.20E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg544_2"
                     /protein_end="72"
                     /protein_start="53"
                     /score="27.6"
                     /tool="antismash"
                     /translation="VFQYTSGSTGKPKGVMLTHG"
     CDS_motif       1093..1170
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg544_2_0002"
                     /evalue="3.20E-09"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg544_2"
                     /protein_end="306"
                     /protein_start="280"
                     /score="29.1"
                     /tool="antismash"
                     /translation="PGQVGEIWVSGQSIAQGYWNCSEQTKL"
     CDS_motif       1264..1326
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg544_2_0003"
                     /evalue="1.30E-05"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg544_2"
                     /protein_end="358"
                     /protein_start="337"
                     /score="17.8"
                     /tool="antismash"
                     /translation="TGRIKDLIIIRGRNHYPQDIEQ"
     aSDomain        1990..2166
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg544_2_ACP.1"
                     /evalue="1.50E-18"
                     /label="ctg544_2_ACP.1"
                     /locus_tag="ctg544_2"
                     /protein_end="638"
                     /protein_start="579"
                     /score="58.6"
                     /tool="antismash"
                     /translation="ATHLNVDDIDIQKPLTTYGLDSLALIELSAQLENWLGFGLSPTTL
                     YEYPTIKTLAEYLDS"
     CDS             2279..3658
                     /NRPS_PKS="Domain: Aminotran_1_2 (104-443). E-value:
                     1.1e-50. Score: 164.4. Matches aSDomain:
                     nrpspksdomains_ctg544_3_Aminotran_1_2.1"
                     /NRPS_PKS="type: other"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109:8-amino-7-oxononanoate synthase (Score: 561;
                     E-value: 2.5e-170)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg544_3"
                     /transl_table=11
                     /translation="MSTSNLSQDISSLSTAEKRQLIKKLISQRATDLHNCDLSNLETFF
                     SQLLGNSKDIPPEYYNFSLYPDYRKLIEQIQGMKDIGAFNPYFTMHDGLNNHITVINGQ
                     EFINYSSYNYIGLSGDPRVSAFAQEAISHFGTSVSASRIVSGERPLHRKLETELAKFIG
                     TEDCIVYVGGHSTNVTTIGHLFGEDDLIIHDALIHNSALEGCKLSGAKRISFPHNNWEE
                     LDRILQERRHKYKRVLLVIEGIYSMDGDIPDLPKFIEIKKHHKTFLMVDEAHSIGVLGE
                     HGRGIGEYFGINPADVDLWMGTLSKSFASCGGYIAGCKALVEYLKYSAPGFVYSVGMSP
                     PNAAAALAAINVLKSERERVKCLYQRSKLFLELASQKGLNTGMSRNTPIVPVILGNSQQ
                     CIQLSQLLFEQGINVRPIIYPAVPDKEARLRFFITSTHTEEQIRFTVNTVAEKLTQTKA
                     Y"
     aSDomain        2591..3607
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg544_3_Aminotran_1_2.1"
                     /evalue="1.10E-50"
                     /label="ctg544_3_Aminotran_1_2.1"
                     /locus_tag="ctg544_3"
                     /protein_end="443"
                     /protein_start="104"
                     /score="164.4"
                     /tool="antismash"
                     /translation="EFINYSSYNYIGLSGDPRVSAFAQEAISHFGTSVSASRIVSGERP
                     LHRKLETELAKFIGTEDCIVYVGGHSTNVTTIGHLFGEDDLIIHDALIHNSALEGCKLS
                     GAKRISFPHNNWEELDRILQERRHKYKRVLLVIEGIYSMDGDIPDLPKFIEIKKHHKTF
                     LMVDEAHSIGVLGEHGRGIGEYFGINPADVDLWMGTLSKSFASCGGYIAGCKALVEYLK
                     YSAPGFVYSVGMSPPNAAAALAAINVLKSERERVKCLYQRSKLFLELASQKGLNTGMSR
                     NTPIVPVILGNSQQCIQLSQLLFEQGINVRPIIYPAVPDKEARLRFFITSTHTEEQIRF
                     "
     CDS             3805..4683
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 78.4; E-value:
                     9.8e-24)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg544_4"
                     /sec_met_domain="PCMT (E-value: 6.6e-11, bitscore: 30.4,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSALKLPKLLYRTAILKLSHNNSDPVTDYDNAAASYDEYYSKYL
                     GKRALEMLEKLPIKPGDCILDLACGTGVFSHPLAEQVGESGTIVAVDISPGMLQQNREK
                     AMIKNLSNINFVESDALSFLQRVSSNSVDGVVCAWGICYMNHEQLRQELERVVKPGGFI
                     GLIENRACTLKDVSDLFTKVLMDYPEAIEKNMVLHLPKDHKYLVKAFCKNRFKALEALD
                     DFVVVPCKNGNEIVDYMVKSGASAGFINALEASMVNTVLQKLIEYANEGMSRGRKILVK
                     HEFSILIGEKI"
ORIGIN
        1 aaaacttcaa atgttggcat ttgcctcaaa taaaacaggc ataaacatat ctcttctttt
       61 acctccagtt ttggtttacg acctcctcct ttctcattta tacgtatttt tttacgttca
      121 atctcagcct gaattttatt atgatgtatt tcagcctggt ttaataattc ttgaaattgc
      181 tcgttgctta ttcccagtat ttgttttgtg cgctgtgggt attcttgaat ataatgaagt
      241 ggatttttca ttttggtaga cggtgaaaac tccgttctac catttttttg gcaccaagtt
      301 aatattccgg acaggtctaa tatagaaaat gatttagatg aaaatgattt agatgaaaat
      361 aatttaaccc gagaatggcg tgaaccaatt acaaacagtc atacactagc agtttttcaa
      421 tacacttcag gttctacagg taaacccaaa ggagtaatgt taacccatgg gaacttatta
      481 cataattccg ctctaattca tcagtgtttt ggacatagta aagacagtca aggtgtaatt
      541 tggttaccac cttatcatga tatgggtcta atcggaggaa ttatacaacc cctgtatgga
      601 ggttttcccg ttaccctgat gtctccagtt gatttcttac aaaaacccta tcgttggtta
      661 caagcaattt ctcggtataa ggctactacg agtggtggtc ctaattttgc ttatgacttg
      721 tgcatgcata aaataactga agaacagcga gcaactctcg atctaagtag ttgggaagtc
      781 gctttcaatg gagccgaacc tattcgtgca gagactttag aacaatttgt tgcagtattt
      841 gaaccttgtg gttttcgtaa agaagccttt tacccctgct atggaatggc tgaatcgacc
      901 ttaattatat ctggtggttt aaaagattct cagccagttt acaagactat tcaagccaat
      961 aggttagagg aaaatcgagt agttttaaca acaaaagata accataaaaa tcttactcta
     1021 gtcagttgcg gacaaactag aatgaaccaa aaaattacga tcgcagatcc ggaaaaatta
     1081 acatcttgtt ccccgggtca agttggtgaa atttgggtat caggtcaaag tatcgctcaa
     1141 ggatattgga attgttccga gcaaacaaag ctcaattttc aagcttattt attggacagt
     1201 ggagagggac catttttacg aactggagat ctaggattta tagaagatgg tgagttatat
     1261 attactggac ggatcaaaga cttaataatt attcggggtc gcaatcacta tcctcaagat
     1321 atcgaacaga cagttcaaac tagtcaccca gctttaaaaa tgggtcatgg agcagcattt
     1381 tcagttgaag tagaaggaaa tgaaacatta gtaattaccc aagaagtaaa acgtagttat
     1441 ttacgcaaac taaatatcaa tcaagtagtt acagctattt gtcaagcagt agcacaagaa
     1501 catgaaatcc aagtatctgg gattttgtta ctaaagactg gaagcatacc taagacttct
     1561 agcggtaaaa ttcaacgtta tgcttgtcgt caaggtttta tgaataatac tttgaatgtt
     1621 gtatcagatt ggacttcaag tccgagaatg aaatctgagt ttcggaattt acaagcagaa
     1681 ttaaatcaaa tagagcaaga tttgcacact aatcgatcgc cccatcaatc cctcataaaa
     1741 tctgattctc aagtcaatgg tcaaggaaac tatggagaaa atattcaaac tcaaagaata
     1801 tctgtagtta aaccacctaa ttcctacttt tctcaaacag aagagactca aattcaaaat
     1861 atatctgtaa cgcagattca agaacagaac acttgcgata atcaggaaat gctttccagt
     1921 tcccataaca gcttagaagc aattcagaat ttgttagtag ttaggattat tgattatctc
     1981 aaagctaatg cgacccatct taatgttgat gatatagata tccaaaaacc cttaaccaca
     2041 tatggattgg actctttagc tttaattgaa ttatcagccc aattagaaaa ttggcttggg
     2101 tttgggcttt ctccgactac tttatatgaa tatcccacca ttaaaactct tgctgaatat
     2161 ctggattctt tgcaaaatca aaaacttgaa gcaaaagtag aagaactctc cgaacaacaa
     2221 atcgattcag tactacaaaa attattggac taaatcaaac tcaataaaag gtagaaccat
     2281 gtcaacgtct aatctatccc aagatatatc cagtttatcc acagcagaaa aacgccaact
     2341 aattaaaaaa ctaatatccc aaagagcaac cgatctccat aattgcgacc tttctaactt
     2401 agaaaccttt tttagtcagt tgttgggaaa cagtaaagac ataccacctg aatattacaa
     2461 cttttccctc tatcccgatt accgcaagct tatcgaacaa attcagggaa tgaaagatat
     2521 aggtgctttc aatccatatt tcaccatgca tgatggttta aataaccaca taaccgtaat
     2581 taacggtcaa gaatttatta attattccag ctacaactac atcggtttat ccggggatcc
     2641 tagagtatcc gcttttgctc aagaagcaat ttcacatttc ggaacatctg tatctgctag
     2701 tcgcattgtt tctggagaaa gaccattaca ccgcaaatta gaaacagagc tagcaaaatt
     2761 cattggtacc gaagactgta tagtttacgt cggaggtcat tccactaacg ttacaaccat
     2821 cggtcattta tttggagaag atgatttaat tatccacgat gcactcattc acaacagtgc
     2881 tttagaaggt tgtaaattat ctggagccaa aagaatttca ttcccccaca acaattggga
     2941 ggaactagat cggatcctcc aagagcgaag acataaatac aaacgggtat tattagtaat
     3001 tgaaggtatc tatagtatgg atggagatat tcccgatcta ccaaaattta ttgaaattaa
     3061 aaaacatcat aagacattcc taatggtaga tgaagcccat tccatcggtg ttttaggaga
     3121 acatggtcgt gggatcggtg aatattttgg aataaatcct gcagatgtgg atttatggat
     3181 gggtacccta agtaaatctt ttgcaagttg tgggggttac atagctggtt gcaaagcttt
     3241 agtggagtat ctcaaatatt cagcaccagg atttgtgtat agcgttggga tgtcaccacc
     3301 aaatgcagca gcagctcttg cagcaattaa tgtcctaaaa agcgagcgag aaagagttaa
     3361 atgtttgtat cagcgctcaa agctattttt agaacttgct agtcagaaag gacttaatac
     3421 gggtatgagt cgtaatactc caatcgtacc ggtgatttta ggtaactctc aacagtgtat
     3481 ccaactttct caactcctct ttgaacaagg tattaacgtt agacctatta tctatcctgc
     3541 agttccagat aaagaagctc gtctgcggtt tttcatcact tccacacata cagaagaaca
     3601 gattcgtttc accgtaaata cagtagccga aaaattaact caaaccaaag catattaatt
     3661 ttacagactt tgtttttcca caaaatcctg tagagactta ccaaaacctg aaccgcacct
     3721 acggtgaatg gtgtcggtgt agcctttggt aggtctgtac aatgatgtat ttttacatcg
     3781 aaataaagca attgaggtaa accaatgaaa tcagcattaa aactaccaaa acttttgtat
     3841 agaacagcca tcctcaaact gagtcataat aactcagacc ccgtgactga ttatgacaat
     3901 gctgctgcat cctacgacga atattactcc aaatatctgg ggaaacgggc gttagaaatg
     3961 ttagaaaaac tacccataaa gccgggagat tgtattttag atttagcttg tggtactggc
     4021 gtttttagcc atccattagc agaacaagtt ggagaatcgg gaacaattgt tgcagtagat
     4081 atttccccag ggatgctaca acaaaatcga gagaaagcaa tgattaaaaa cctatcaaat
     4141 attaactttg ttgaatcaga tgctctttca tttttacaac gagtttcctc caactcggtt
     4201 gatggtgtag tttgtgcttg gggaatttgc tacatgaatc acgagcaatt acgacaggaa
     4261 ttagaaaggg tagttaaacc cggtggtttt attggtttaa ttgaaaatcg agcttgcacc
     4321 ctcaaagatg tatccgatct atttaccaaa gttcttatgg attatccaga agcaatagaa
     4381 aaaaatatgg ttttacattt acctaaagac cataaatatt tagtcaaagc tttttgtaaa
     4441 aataggttta aagctctaga agcattagat gattttgttg tagtaccttg taaaaatggg
     4501 aatgaaatcg tagattacat ggtcaaatca ggggcttcag ccggttttat aaatgcatta
     4561 gaagcatcaa tggtaaatac agttttgcaa aaattaattg aatatgcaaa tgaaggaatg
     4621 tctaggggaa gaaagatatt agtcaaacat gaattttcta ttttgattgg tgaaaaaatt
     4681 taatactatt aggagtggga tatatttccc acttgtaatc atctttttat ccccaataaa
     4741 atttaaaaat aattactaac taggtaatca aagtaatttg tatatgcata attgatttct
     4801 attcgtaatt acccattacc cattacccat tacca
//
