3300008012_33 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00024_3300008.. (original name was: 3300008012.a:Ga0075480_10000323)

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	T1PKS,NRPS	1	27,554

c00146_3300008.. (original name was: 3300008012.a:Ga0075480_10000540)

Region	Type	From	To	Most similar known cluster		Similarity
Region 146.1	NRPS,T1PKS	1	22,821

c00170_3300008.. (original name was: 3300008012.a:Ga0075480_10000213)

Region	Type	From	To	Most similar known cluster		Similarity
Region 170.1	NRPS-like,T1PKS	1	32,486

c00174_3300008.. (original name was: 3300008012.a:Ga0075480_10000740)

Region	Type	From	To	Most similar known cluster		Similarity
Region 174.1	T1PKS,NRPS	1	19,638

c00198_3300008.. (original name was: 3300008012.a:Ga0075480_10011531)

Region	Type	From	To	Most similar known cluster		Similarity
Region 198.1	bacteriocin	1	5,526

Region	Type	From	To	Similarity
Region 24.1	T1PKS,NRPS	1	27,554
Region 146.1	NRPS,T1PKS	1	22,821
Region 170.1	NRPS-like,T1PKS	1	32,486
Region 174.1	T1PKS,NRPS	1	19,638
Region 198.1	bacteriocin	1	5,526

No secondary metabolite regions were found in these records:: c00001_3300008.. (original name was: 3300008012.a:Ga0075480_10019500); c00002_3300008.. (original name was: 3300008012.a:Ga0075480_10019890); c00003_3300008.. (original name was: 3300008012.a:Ga0075480_10009683); c00004_3300008.. (original name was: 3300008012.a:Ga0075480_10002326); c00005_3300008.. (original name was: 3300008012.a:Ga0075480_10010523); c00006_3300008.. (original name was: 3300008012.a:Ga0075480_10012278); c00007_3300008.. (original name was: 3300008012.a:Ga0075480_10005710); c00008_3300008.. (original name was: 3300008012.a:Ga0075480_10022070); c00009_3300008.. (original name was: 3300008012.a:Ga0075480_10016400); c00010_3300008.. (original name was: 3300008012.a:Ga0075480_10004177); c00011_3300008.. (original name was: 3300008012.a:Ga0075480_10031084); c00012_3300008.. (original name was: 3300008012.a:Ga0075480_10011931); c00013_3300008.. (original name was: 3300008012.a:Ga0075480_10017634); c00014_3300008.. (original name was: 3300008012.a:Ga0075480_10026312); c00015_3300008.. (original name was: 3300008012.a:Ga0075480_10014142); c00016_3300008.. (original name was: 3300008012.a:Ga0075480_10018772); c00017_3300008.. (original name was: 3300008012.a:Ga0075480_10024706); c00018_3300008.. (original name was: 3300008012.a:Ga0075480_10014320); c00019_3300008.. (original name was: 3300008012.a:Ga0075480_10027826); c00020_3300008.. (original name was: 3300008012.a:Ga0075480_10033934); c00021_3300008.. (original name was: 3300008012.a:Ga0075480_10013127); c00022_3300008.. (original name was: 3300008012.a:Ga0075480_10003142); c00023_3300008.. (original name was: 3300008012.a:Ga0075480_10003725); c00025_3300008.. (original name was: 3300008012.a:Ga0075480_10001959); c00026_3300008.. (original name was: 3300008012.a:Ga0075480_10025068); c00027_3300008.. (original name was: 3300008012.a:Ga0075480_10002690); c00028_3300008.. (original name was: 3300008012.a:Ga0075480_10013207); c00029_3300008.. (original name was: 3300008012.a:Ga0075480_10007254); c00030_3300008.. (original name was: 3300008012.a:Ga0075480_10001810); c00031_3300008.. (original name was: 3300008012.a:Ga0075480_10003503); c00032_3300008.. (original name was: 3300008012.a:Ga0075480_10016882); c00033_3300008.. (original name was: 3300008012.a:Ga0075480_10002833); c00034_3300008.. (original name was: 3300008012.a:Ga0075480_10016125); c00035_3300008.. (original name was: 3300008012.a:Ga0075480_10010268); c00036_3300008.. (original name was: 3300008012.a:Ga0075480_10006885); c00037_3300008.. (original name was: 3300008012.a:Ga0075480_10008677); c00038_3300008.. (original name was: 3300008012.a:Ga0075480_10017185); c00039_3300008.. (original name was: 3300008012.a:Ga0075480_10010720); c00040_3300008.. (original name was: 3300008012.a:Ga0075480_10017138); c00041_3300008.. (original name was: 3300008012.a:Ga0075480_10013711); c00042_3300008.. (original name was: 3300008012.a:Ga0075480_10029532); c00043_3300008.. (original name was: 3300008012.a:Ga0075480_10029404); c00044_3300008.. (original name was: 3300008012.a:Ga0075480_10021270); c00045_3300008.. (original name was: 3300008012.a:Ga0075480_10030840); c00046_3300008.. (original name was: 3300008012.a:Ga0075480_10024091); c00047_3300008.. (original name was: 3300008012.a:Ga0075480_10019341); c00048_3300008.. (original name was: 3300008012.a:Ga0075480_10030073); c00049_3300008.. (original name was: 3300008012.a:Ga0075480_10003455); c00050_3300008.. (original name was: 3300008012.a:Ga0075480_10002950); c00051_3300008.. (original name was: 3300008012.a:Ga0075480_10000860); c00052_3300008.. (original name was: 3300008012.a:Ga0075480_10021144); c00053_3300008.. (original name was: 3300008012.a:Ga0075480_10015501); c00054_3300008.. (original name was: 3300008012.a:Ga0075480_10005313); c00055_3300008.. (original name was: 3300008012.a:Ga0075480_10012993); c00056_3300008.. (original name was: 3300008012.a:Ga0075480_10001580); c00057_3300008.. (original name was: 3300008012.a:Ga0075480_10014840); c00058_3300008.. (original name was: 3300008012.a:Ga0075480_10004828); c00059_3300008.. (original name was: 3300008012.a:Ga0075480_10020589); c00060_3300008.. (original name was: 3300008012.a:Ga0075480_10020532); c00061_3300008.. (original name was: 3300008012.a:Ga0075480_10020023); c00062_3300008.. (original name was: 3300008012.a:Ga0075480_10007116); c00063_3300008.. (original name was: 3300008012.a:Ga0075480_10009986); c00064_3300008.. (original name was: 3300008012.a:Ga0075480_10001481); c00065_3300008.. (original name was: 3300008012.a:Ga0075480_10001551); c00066_3300008.. (original name was: 3300008012.a:Ga0075480_10000705); c00067_3300008.. (original name was: 3300008012.a:Ga0075480_10009494); c00068_3300008.. (original name was: 3300008012.a:Ga0075480_10005772); c00069_3300008.. (original name was: 3300008012.a:Ga0075480_10023675); c00070_3300008.. (original name was: 3300008012.a:Ga0075480_10008987); c00071_3300008.. (original name was: 3300008012.a:Ga0075480_10024726); c00072_3300008.. (original name was: 3300008012.a:Ga0075480_10023185); c00073_3300008.. (original name was: 3300008012.a:Ga0075480_10015839); c00074_3300008.. (original name was: 3300008012.a:Ga0075480_10011708); c00075_3300008.. (original name was: 3300008012.a:Ga0075480_10014183); c00076_3300008.. (original name was: 3300008012.a:Ga0075480_10005496); c00077_3300008.. (original name was: 3300008012.a:Ga0075480_10000713); c00078_3300008.. (original name was: 3300008012.a:Ga0075480_10028636); c00079_3300008.. (original name was: 3300008012.a:Ga0075480_10014691); c00080_3300008.. (original name was: 3300008012.a:Ga0075480_10009633); c00081_3300008.. (original name was: 3300008012.a:Ga0075480_10028211); c00082_3300008.. (original name was: 3300008012.a:Ga0075480_10009755); c00083_3300008.. (original name was: 3300008012.a:Ga0075480_10000569); c00084_3300008.. (original name was: 3300008012.a:Ga0075480_10011216); c00085_3300008.. (original name was: 3300008012.a:Ga0075480_10018279); c00086_3300008.. (original name was: 3300008012.a:Ga0075480_10008982); c00087_3300008.. (original name was: 3300008012.a:Ga0075480_10003981); c00088_3300008.. (original name was: 3300008012.a:Ga0075480_10006419); c00089_3300008.. (original name was: 3300008012.a:Ga0075480_10015352); c00090_3300008.. (original name was: 3300008012.a:Ga0075480_10029157); c00091_3300008.. (original name was: 3300008012.a:Ga0075480_10003619); c00092_3300008.. (original name was: 3300008012.a:Ga0075480_10002019); c00093_3300008.. (original name was: 3300008012.a:Ga0075480_10017049); c00094_3300008.. (original name was: 3300008012.a:Ga0075480_10009748); c00095_3300008.. (original name was: 3300008012.a:Ga0075480_10006416); c00096_3300008.. (original name was: 3300008012.a:Ga0075480_10012127); c00097_3300008.. (original name was: 3300008012.a:Ga0075480_10003653); c00098_3300008.. (original name was: 3300008012.a:Ga0075480_10005954); c00099_3300008.. (original name was: 3300008012.a:Ga0075480_10009540); c00100_3300008.. (original name was: 3300008012.a:Ga0075480_10022118); c00101_3300008.. (original name was: 3300008012.a:Ga0075480_10012673); c00102_3300008.. (original name was: 3300008012.a:Ga0075480_10032879); c00103_3300008.. (original name was: 3300008012.a:Ga0075480_10012438); c00104_3300008.. (original name was: 3300008012.a:Ga0075480_10029409); c00105_3300008.. (original name was: 3300008012.a:Ga0075480_10022406); c00106_3300008.. (original name was: 3300008012.a:Ga0075480_10030255); c00107_3300008.. (original name was: 3300008012.a:Ga0075480_10004551); c00108_3300008.. (original name was: 3300008012.a:Ga0075480_10003755); c00109_3300008.. (original name was: 3300008012.a:Ga0075480_10017502); c00110_3300008.. (original name was: 3300008012.a:Ga0075480_10011602); c00111_3300008.. (original name was: 3300008012.a:Ga0075480_10019052); c00112_3300008.. (original name was: 3300008012.a:Ga0075480_10012694); c00113_3300008.. (original name was: 3300008012.a:Ga0075480_10007714); c00114_3300008.. (original name was: 3300008012.a:Ga0075480_10017841); c00115_3300008.. (original name was: 3300008012.a:Ga0075480_10020388); c00116_3300008.. (original name was: 3300008012.a:Ga0075480_10020113); c00117_3300008.. (original name was: 3300008012.a:Ga0075480_10020605); c00118_3300008.. (original name was: 3300008012.a:Ga0075480_10001825); c00119_3300008.. (original name was: 3300008012.a:Ga0075480_10026933); c00120_3300008.. (original name was: 3300008012.a:Ga0075480_10001055); c00121_3300008.. (original name was: 3300008012.a:Ga0075480_10004615); c00122_3300008.. (original name was: 3300008012.a:Ga0075480_10016014); c00123_3300008.. (original name was: 3300008012.a:Ga0075480_10008794); c00124_3300008.. (original name was: 3300008012.a:Ga0075480_10010522); c00125_3300008.. (original name was: 3300008012.a:Ga0075480_10009185); c00126_3300008.. (original name was: 3300008012.a:Ga0075480_10031955); c00127_3300008.. (original name was: 3300008012.a:Ga0075480_10009715); c00128_3300008.. (original name was: 3300008012.a:Ga0075480_10015156); c00129_3300008.. (original name was: 3300008012.a:Ga0075480_10008964); c00130_3300008.. (original name was: 3300008012.a:Ga0075480_10026172); c00131_3300008.. (original name was: 3300008012.a:Ga0075480_10010341); c00132_3300008.. (original name was: 3300008012.a:Ga0075480_10003845); c00133_3300008.. (original name was: 3300008012.a:Ga0075480_10002268); c00134_3300008.. (original name was: 3300008012.a:Ga0075480_10009848); c00135_3300008.. (original name was: 3300008012.a:Ga0075480_10012338); c00136_3300008.. (original name was: 3300008012.a:Ga0075480_10004017); c00137_3300008.. (original name was: 3300008012.a:Ga0075480_10009768); c00138_3300008.. (original name was: 3300008012.a:Ga0075480_10009666); c00139_3300008.. (original name was: 3300008012.a:Ga0075480_10021358); c00140_3300008.. (original name was: 3300008012.a:Ga0075480_10001149); c00141_3300008.. (original name was: 3300008012.a:Ga0075480_10012505); c00142_3300008.. (original name was: 3300008012.a:Ga0075480_10005481); c00143_3300008.. (original name was: 3300008012.a:Ga0075480_10028607); c00144_3300008.. (original name was: 3300008012.a:Ga0075480_10019970); c00145_3300008.. (original name was: 3300008012.a:Ga0075480_10013073); c00147_3300008.. (original name was: 3300008012.a:Ga0075480_10009134); c00148_3300008.. (original name was: 3300008012.a:Ga0075480_10012039); c00149_3300008.. (original name was: 3300008012.a:Ga0075480_10004427); c00150_3300008.. (original name was: 3300008012.a:Ga0075480_10019181); c00151_3300008.. (original name was: 3300008012.a:Ga0075480_10009160); c00152_3300008.. (original name was: 3300008012.a:Ga0075480_10020433); c00153_3300008.. (original name was: 3300008012.a:Ga0075480_10021132); c00154_3300008.. (original name was: 3300008012.a:Ga0075480_10025683); c00155_3300008.. (original name was: 3300008012.a:Ga0075480_10002673); c00156_3300008.. (original name was: 3300008012.a:Ga0075480_10025850); c00157_3300008.. (original name was: 3300008012.a:Ga0075480_10006229); c00158_3300008.. (original name was: 3300008012.a:Ga0075480_10015166); c00159_3300008.. (original name was: 3300008012.a:Ga0075480_10034689); c00160_3300008.. (original name was: 3300008012.a:Ga0075480_10000592); c00161_3300008.. (original name was: 3300008012.a:Ga0075480_10030361); c00162_3300008.. (original name was: 3300008012.a:Ga0075480_10017753); c00163_3300008.. (original name was: 3300008012.a:Ga0075480_10019213); c00164_3300008.. (original name was: 3300008012.a:Ga0075480_10016363); c00165_3300008.. (original name was: 3300008012.a:Ga0075480_10028029); c00166_3300008.. (original name was: 3300008012.a:Ga0075480_10000396); c00167_3300008.. (original name was: 3300008012.a:Ga0075480_10032618); c00168_3300008.. (original name was: 3300008012.a:Ga0075480_10023142); c00169_3300008.. (original name was: 3300008012.a:Ga0075480_10025237); c00171_3300008.. (original name was: 3300008012.a:Ga0075480_10012486); c00172_3300008.. (original name was: 3300008012.a:Ga0075480_10034621); c00173_3300008.. (original name was: 3300008012.a:Ga0075480_10012387); c00175_3300008.. (original name was: 3300008012.a:Ga0075480_10027253); c00176_3300008.. (original name was: 3300008012.a:Ga0075480_10026839); c00177_3300008.. (original name was: 3300008012.a:Ga0075480_10014581); c00178_3300008.. (original name was: 3300008012.a:Ga0075480_10030869); c00179_3300008.. (original name was: 3300008012.a:Ga0075480_10031508); c00180_3300008.. (original name was: 3300008012.a:Ga0075480_10007493); c00181_3300008.. (original name was: 3300008012.a:Ga0075480_10016448); c00182_3300008.. (original name was: 3300008012.a:Ga0075480_10012765); c00183_3300008.. (original name was: 3300008012.a:Ga0075480_10020886); c00184_3300008.. (original name was: 3300008012.a:Ga0075480_10020022); c00185_3300008.. (original name was: 3300008012.a:Ga0075480_10002812); c00186_3300008.. (original name was: 3300008012.a:Ga0075480_10033726); c00187_3300008.. (original name was: 3300008012.a:Ga0075480_10032086); c00188_3300008.. (original name was: 3300008012.a:Ga0075480_10007532); c00189_3300008.. (original name was: 3300008012.a:Ga0075480_10009979); c00190_3300008.. (original name was: 3300008012.a:Ga0075480_10024527); c00191_3300008.. (original name was: 3300008012.a:Ga0075480_10030121); c00192_3300008.. (original name was: 3300008012.a:Ga0075480_10027161); c00193_3300008.. (original name was: 3300008012.a:Ga0075480_10034529); c00194_3300008.. (original name was: 3300008012.a:Ga0075480_10016243); c00195_3300008.. (original name was: 3300008012.a:Ga0075480_10028384); c00196_3300008.. (original name was: 3300008012.a:Ga0075480_10004125); c00197_3300008.. (original name was: 3300008012.a:Ga0075480_10028079); c00199_3300008.. (original name was: 3300008012.a:Ga0075480_10010159); c00200_3300008.. (original name was: 3300008012.a:Ga0075480_10031907); c00201_3300008.. (original name was: 3300008012.a:Ga0075480_10015820); c00202_3300008.. (original name was: 3300008012.a:Ga0075480_10016979); c00203_3300008.. (original name was: 3300008012.a:Ga0075480_10007042)

Compact view

c00024_3300008.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 1 - 27,554 nt. (total: 27,554 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 27554:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal) + (mal - asn) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg24_7

PKS_AT (533..821): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.6
inactive: 76.2
Methoxymalonyl-CoA: 53.5
Methylmalonyl-CoA: 36.9
Benzoyl-CoA: 20.1
Propionyl-CoA: 20.0
2-Methylbutyryl-CoA: 19.3
CHC-CoA: 18.0
fatty_acid: 17.1
Acetyl-CoA: 16.4
Isobutyryl-CoA: 15.9
trans-1,2-CPDA: 10.0
Ethylmalonyl-CoA: 6.9
3-Methylbutyryl-CoA: 0.0

ctg24_8

Search NORINE for peptide: strict or relaxed

PKS_AT (568..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.9
inactive: 73.9
Methylmalonyl-CoA: 70.7
Methoxymalonyl-CoA: 67.1
Propionyl-CoA: 51.3
Ethylmalonyl-CoA: 48.6
Isobutyryl-CoA: 44.0
2-Methylbutyryl-CoA: 32.6
Acetyl-CoA: 19.4
CHC-CoA: 19.3
Benzoyl-CoA: 19.1
fatty_acid: 18.9
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 9.0

AMP-binding (2413..2820): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DApKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)

ctg24_9

PKS_AT (335..630): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 152.2
inactive: 73.7
Methoxymalonyl-CoA: 72.1
Methylmalonyl-CoA: 63.7
Propionyl-CoA: 48.5
Ethylmalonyl-CoA: 35.0
Isobutyryl-CoA: 34.3
fatty_acid: 24.6
Benzoyl-CoA: 24.2
2-Methylbutyryl-CoA: 24.1
CHC-CoA: 23.1
Acetyl-CoA: 22.2
3-Methylbutyryl-CoA: 18.9
trans-1,2-CPDA: 18.6

ctg24_11

PKS_AT (1..168): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 54.5
inactive: 25.0
Methoxymalonyl-CoA: 20.5
Methylmalonyl-CoA: 15.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

c00146_3300008.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 1 - 22,821 nt. (total: 22,821 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 22821:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asn) + (pk) + (thr - gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg146_1

Search NORINE for peptide: strict or relaxed

AMP-binding (82..488): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1488..1874): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DIpQLGfVWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 80% (moderate)

ctg146_2

PKS_AT (481..757): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 30.6
Propionyl-CoA: 14.2
Malonyl-CoA: 13.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg146_3

Search NORINE for peptide: strict or relaxed

AMP-binding (1134..1532): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg146_8

PKS_AT (18..258): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 58.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 79.7
inactive: 44.9
Methoxymalonyl-CoA: 42.5
Methylmalonyl-CoA: 34.5
Propionyl-CoA: 28.2
Ethylmalonyl-CoA: 16.7
3-Methylbutyryl-CoA: 14.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

c00170_3300008.. - Region 1 - NRPS-like,T1PKS

Download region GenBank file

Location: 1 - 32,486 nt. (total: 32,486 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 32486:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (ccmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg170_11

CAL_domain (108..430): pk

minowa: NH2

Prediction, score:

NH2: 112.6
Acetyl-CoA: 72.9
fatty_acid: 40.9
AHBA: 11.7
shikimic_acid: 0.0

PKS_AT (1156..1438): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.0
Methoxymalonyl-CoA: 51.6
inactive: 45.6
Methylmalonyl-CoA: 41.6
Propionyl-CoA: 24.6
Ethylmalonyl-CoA: 23.9
2-Methylbutyryl-CoA: 20.6
CHC-CoA: 15.7
Benzoyl-CoA: 12.4
trans-1,2-CPDA: 10.5
Isobutyryl-CoA: 10.3
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg170_12

PKS_AT (569..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.5
Methoxymalonyl-CoA: 80.9
Methylmalonyl-CoA: 71.3
inactive: 69.6
Propionyl-CoA: 51.0
Ethylmalonyl-CoA: 34.5
fatty_acid: 22.2
Isobutyryl-CoA: 19.9
Acetyl-CoA: 18.0
CHC-CoA: 15.5
2-Methylbutyryl-CoA: 14.4
trans-1,2-CPDA: 14.3
Benzoyl-CoA: 10.8
3-Methylbutyryl-CoA: 9.7

ctg170_13

PKS_AT (559..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 62.5%; CE-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 103.9
Methylmalonyl-CoA: 78.1
Methoxymalonyl-CoA: 69.3
inactive: 59.5
Isobutyryl-CoA: 46.2
Propionyl-CoA: 44.4
Ethylmalonyl-CoA: 38.5
Acetyl-CoA: 28.0
2-Methylbutyryl-CoA: 24.2
CHC-CoA: 21.1
Benzoyl-CoA: 20.2
fatty_acid: 18.4
trans-1,2-CPDA: 13.2
3-Methylbutyryl-CoA: 7.2

c00174_3300008.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 1 - 19,638 nt. (total: 19,638 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 19638:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X) + (gly - ohmal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg174_1

Search NORINE for peptide: strict or relaxed

AMP-binding (826..1218): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVGvIGeVmK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 70% (weak)

ctg174_2

Search NORINE for peptide: strict or relaxed

AMP-binding (534..930): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

PKS_AT (1641..1928): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.5
inactive: 48.1
Methoxymalonyl-CoA: 43.9
Methylmalonyl-CoA: 30.2
Propionyl-CoA: 22.4
2-Methylbutyryl-CoA: 17.9
Benzoyl-CoA: 12.9
Ethylmalonyl-CoA: 10.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg174_3

Search NORINE for peptide: strict or relaxed

AMP-binding (45..471): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: nIyWLAASgK
Nearest Stachelhaus code:: 2-oxo-isovaleric-acid
Stachelhaus code match:: 70% (weak)