3300013732_1 - 10 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00001_3300013.. (original name was: 3300013732.a:Ga0121191_100033)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	betalactone	1,448	24,600

c00006_3300013.. (original name was: 3300013732.a:Ga0121191_100018)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS	63,011	99,949

c00009_3300013.. (original name was: 3300013732.a:Ga0121191_100001)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	NAGGN	696,468	711,144

c00013_3300013.. (original name was: 3300013732.a:Ga0121191_100005)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	butyrolactone	127,316	138,212

c00017_3300013.. (original name was: 3300013732.a:Ga0121191_100007)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	CDPS	19,443	40,180

c00019_3300013.. (original name was: 3300013732.a:Ga0121191_100009)

Region	Type	From	To
Region 19.1	NRPS	17,133	84,527
Region 19.2	bacteriocin	218,491	229,327
Region 19.3	NRPS	239,055	280,835

c00021_3300013.. (original name was: 3300013732.a:Ga0121191_100014)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	phenazine	71,785	93,370
Region 21.2	phenazine	94,087	115,085

Region	Type	From	To	Similarity
Region 1.1	betalactone	1,448	24,600
Region 6.1	NRPS	63,011	99,949
Region 9.1	NAGGN	696,468	711,144
Region 13.1	butyrolactone	127,316	138,212
Region 17.1	CDPS	19,443	40,180
Region 19.1	NRPS	17,133	84,527
Region 19.2	bacteriocin	218,491	229,327
Region 19.3	NRPS	239,055	280,835
Region 21.1	phenazine	71,785	93,370
Region 21.2	phenazine	94,087	115,085

No secondary metabolite regions were found in these records:: c00002_3300013.. (original name was: 3300013732.a:Ga0121191_100026); c00003_3300013.. (original name was: 3300013732.a:Ga0121191_100023); c00004_3300013.. (original name was: 3300013732.a:Ga0121191_100022); c00005_3300013.. (original name was: 3300013732.a:Ga0121191_100019); c00007_3300013.. (original name was: 3300013732.a:Ga0121191_100017); c00008_3300013.. (original name was: 3300013732.a:Ga0121191_100016); c00010_3300013.. (original name was: 3300013732.a:Ga0121191_100025); c00011_3300013.. (original name was: 3300013732.a:Ga0121191_100002); c00012_3300013.. (original name was: 3300013732.a:Ga0121191_100013); c00014_3300013.. (original name was: 3300013732.a:Ga0121191_100029); c00015_3300013.. (original name was: 3300013732.a:Ga0121191_100006); c00016_3300013.. (original name was: 3300013732.a:Ga0121191_100030); c00018_3300013.. (original name was: 3300013732.a:Ga0121191_100032); c00020_3300013.. (original name was: 3300013732.a:Ga0121191_100011); c00022_3300013.. (original name was: 3300013732.a:Ga0121191_100015)

Compact view

c00001_3300013.. - Region 1 - betalactone

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Location: 1,448 - 24,600 nt. (total: 23,153 nt) Show pHMM detection rules used

Region on contig edge.

betalactone: (HMGL-like and AMP-binding)

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c00006_3300013.. - Region 1 - NRPS

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Location: 63,011 - 99,949 nt. (total: 36,939 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 63010 - 99949:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - thr) + (D-ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_71

Search NORINE for peptide: strict or relaxed

AMP-binding (495..905): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeDhGtVtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 50% (weak)

AMP-binding (1566..1974): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg6_72

Search NORINE for peptide: strict or relaxed

AMP-binding (511..908): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (2012..2411): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: N/A
Single AA prediction:: lys

Stachelhaus prediction details:

Stachelhaus sequence:: DgeccGGVtK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

c00009_3300013.. - Region 1 - NAGGN

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Location: 696,468 - 711,144 nt. (total: 14,677 nt) Show pHMM detection rules used

NAGGN: ((TIGR03104 or cds(GATase_7 and Asn_synthase)) and TIGR03103 and (TIGR03106 or Peptidase_M42))

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c00013_3300013.. - Region 1 - butyrolactone

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Location: 127,316 - 138,212 nt. (total: 10,897 nt) Show pHMM detection rules used

butyrolactone: AfsA

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c00017_3300013.. - Region 1 - CDPS

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Location: 19,443 - 40,180 nt. (total: 20,738 nt) Show pHMM detection rules used

CDPS: (CDPS or cycdipepsynth)

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c00019_3300013.. - Region 1 - NRPS

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Location: 17,133 - 84,527 nt. (total: 67,395 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 17132 - 84527:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-X - X) + (D-ala - X) + (asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg19_45

Search NORINE for peptide: strict or relaxed

CAL_domain (45..481): pk

minowa: NH2

Prediction, score:

NH2: 147.3
AHBA: 31.8
fatty_acid: 3.4
shikimic_acid: 2.9
Acetyl-CoA: -1.7

AMP-binding (1136..1530): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFGRINK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (2190..2595): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAegiGAVMK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 70% (weak)

AMP-binding (3715..4121): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg19_47

Search NORINE for peptide: strict or relaxed

AMP-binding (515..905): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLwNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 90% (moderate)

AMP-binding (2009..2417): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAeDhGtVsK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)

ctg19_48

Search NORINE for peptide: strict or relaxed

AMP-binding (450..845): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

c00019_3300013.. - Region 2 - bacteriocin

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Location: 218,491 - 229,327 nt. (total: 10,837 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

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c00019_3300013.. - Region 3 - NRPS

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Location: 239,055 - 280,835 nt. (total: 41,781 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 239054 - 280835:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ala) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg19_246

Search NORINE for peptide: strict or relaxed

AMP-binding (449..842): X

NRPSPredictor2: orn, lys, arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DsKDmGlVnK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 60% (weak)

ctg19_249

Search NORINE for peptide: strict or relaxed

AMP-binding (259..651): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

c00021_3300013.. - Region 1 - phenazine

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Location: 71,785 - 93,370 nt. (total: 21,586 nt) Show pHMM detection rules used

phenazine: phzB

Legend:

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c00021_3300013.. - Region 2 - phenazine

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Location: 94,087 - 115,085 nt. (total: 20,999 nt) Show pHMM detection rules used

phenazine: phzB

Legend:

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