3300017649_12 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00002_3300017.. (original name was: 3300017649.a:Ga0182741_1000053)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	terpene	7,517	28,665

c00011_3300017.. (original name was: 3300017649.a:Ga0182741_1000001)

Region	Type	From	To
Region 11.1	NRPS,T1PKS	325,777	430,081
Region 11.2	T1PKS	432,124	479,938
Region 11.3	terpene	676,727	698,667

c00014_3300017.. (original name was: 3300017649.a:Ga0182741_1000002)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	NRPS-like	820	43,510
Region 14.2	bacteriocin	52,405	63,232

Region	Type	From	To	Similarity
Region 2.1	terpene	7,517	28,665
Region 11.1	NRPS,T1PKS	325,777	430,081
Region 11.2	T1PKS	432,124	479,938
Region 11.3	terpene	676,727	698,667
Region 14.1	NRPS-like	820	43,510
Region 14.2	bacteriocin	52,405	63,232

No secondary metabolite regions were found in these records:: c00001_3300017.. (original name was: 3300017649.a:Ga0182741_1000457); c00003_3300017.. (original name was: 3300017649.a:Ga0182741_1000032); c00004_3300017.. (original name was: 3300017649.a:Ga0182741_1000061); c00005_3300017.. (original name was: 3300017649.a:Ga0182741_1000200); c00006_3300017.. (original name was: 3300017649.a:Ga0182741_1000021); c00007_3300017.. (original name was: 3300017649.a:Ga0182741_1000005); c00008_3300017.. (original name was: 3300017649.a:Ga0182741_1000035); c00009_3300017.. (original name was: 3300017649.a:Ga0182741_1000068); c00010_3300017.. (original name was: 3300017649.a:Ga0182741_1000263); c00012_3300017.. (original name was: 3300017649.a:Ga0182741_1000020); c00013_3300017.. (original name was: 3300017649.a:Ga0182741_1000304)

Compact view

c00011_3300017.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 325,777 - 430,081 nt. (total: 104,305 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 325776 - 430081:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal) + (pk) + (redmal) + (mal) + (redmal) + (ohmal) + (redmal) + (redmal) + (gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 325776 - 377171:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal) + (ohmal) + (redmal) + (redmal) + (gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 338767 - 430081:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal) + (pk) + (redmal) + (mal) + (redmal) + (ohmal) + (redmal) + (redmal) + (gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg11_304

Search NORINE for peptide: strict or relaxed

AMP-binding (10..410): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: aysaTAmarK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 30% (weak)

ctg11_310

Search NORINE for peptide: strict or relaxed

AMP-binding (540..932): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg11_311

PKS_AT (569..862): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 167.0
inactive: 115.3
Methylmalonyl-CoA: 80.2
Methoxymalonyl-CoA: 77.8
Propionyl-CoA: 66.5
Isobutyryl-CoA: 44.2
Benzoyl-CoA: 41.0
Ethylmalonyl-CoA: 40.0
2-Methylbutyryl-CoA: 40.0
CHC-CoA: 28.9
trans-1,2-CPDA: 26.6
Acetyl-CoA: 25.1
fatty_acid: 12.8
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

ctg11_312

PKS_AT (566..864): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.9
inactive: 120.7
Methoxymalonyl-CoA: 72.3
Methylmalonyl-CoA: 68.3
Isobutyryl-CoA: 41.4
Propionyl-CoA: 40.5
Ethylmalonyl-CoA: 40.3
Benzoyl-CoA: 24.4
Acetyl-CoA: 24.3
fatty_acid: 22.5
2-Methylbutyryl-CoA: 22.5
CHC-CoA: 18.9
trans-1,2-CPDA: 16.3
3-Methylbutyryl-CoA: 14.7

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

ctg11_313

PKS_AT (569..869): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.1
inactive: 106.9
Methoxymalonyl-CoA: 86.5
Methylmalonyl-CoA: 83.5
Ethylmalonyl-CoA: 56.7
Isobutyryl-CoA: 49.6
trans-1,2-CPDA: 48.5
Propionyl-CoA: 39.9
Benzoyl-CoA: 38.4
Acetyl-CoA: 32.3
fatty_acid: 30.2
CHC-CoA: 19.2
2-Methylbutyryl-CoA: 17.0
3-Methylbutyryl-CoA: 16.8

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

ctg11_314

PKS_AT (558..854): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.7
inactive: 109.1
Methoxymalonyl-CoA: 93.7
Methylmalonyl-CoA: 84.9
Ethylmalonyl-CoA: 59.7
Isobutyryl-CoA: 53.7
Propionyl-CoA: 52.0
CHC-CoA: 50.9
2-Methylbutyryl-CoA: 42.3
Acetyl-CoA: 34.8
Benzoyl-CoA: 33.2
trans-1,2-CPDA: 33.1
fatty_acid: 28.9
3-Methylbutyryl-CoA: 13.5

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

ctg11_315

PKS_AT (567..868): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.5
inactive: 118.0
Methoxymalonyl-CoA: 72.3
Methylmalonyl-CoA: 63.6
Propionyl-CoA: 51.5
Benzoyl-CoA: 32.5
Isobutyryl-CoA: 30.8
Ethylmalonyl-CoA: 29.7
Acetyl-CoA: 21.5
2-Methylbutyryl-CoA: 19.4
fatty_acid: 18.9
CHC-CoA: 17.2
3-Methylbutyryl-CoA: 11.8
trans-1,2-CPDA: 7.5

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

ctg11_316

PKS_AT (572..867): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.2
inactive: 148.7
Methoxymalonyl-CoA: 69.9
Methylmalonyl-CoA: 64.2
Propionyl-CoA: 55.0
2-Methylbutyryl-CoA: 30.4
Ethylmalonyl-CoA: 26.1
Isobutyryl-CoA: 24.0
CHC-CoA: 23.3
fatty_acid: 18.0
Benzoyl-CoA: 17.5
trans-1,2-CPDA: 16.3
Acetyl-CoA: 8.5
3-Methylbutyryl-CoA: 8.1

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

ctg11_317

PKS_AT (564..861): pk

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 140.0
Malonyl-CoA: 139.5
Methoxymalonyl-CoA: 127.1
Propionyl-CoA: 96.3
Ethylmalonyl-CoA: 96.3
Isobutyryl-CoA: 95.2
2-Methylbutyryl-CoA: 92.2
CHC-CoA: 69.2
Acetyl-CoA: 68.9
inactive: 65.0
trans-1,2-CPDA: 64.1
Benzoyl-CoA: 52.2
3-Methylbutyryl-CoA: 46.8
fatty_acid: 44.9

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 70.8%; Isobutyryl-CoA: 62.5%

ctg11_318

PKS_AT (1350..1648): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.4
inactive: 143.5
Methoxymalonyl-CoA: 62.3
Methylmalonyl-CoA: 55.4
Propionyl-CoA: 42.1
fatty_acid: 22.8
Ethylmalonyl-CoA: 21.2
Isobutyryl-CoA: 18.4
Benzoyl-CoA: 17.9
CHC-CoA: 17.5
Acetyl-CoA: 14.4
2-Methylbutyryl-CoA: 5.4
trans-1,2-CPDA: 5.3
3-Methylbutyryl-CoA: -1.4

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

c00011_3300017.. - Region 2 - T1PKS

Download region GenBank file

Location: 432,124 - 479,938 nt. (total: 47,815 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 4, location 432123 - 479938:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg11_350

PKS_AT (1261..1554): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 148.1
inactive: 103.7
Methylmalonyl-CoA: 88.5
Methoxymalonyl-CoA: 83.1
Propionyl-CoA: 74.0
2-Methylbutyryl-CoA: 58.4
Isobutyryl-CoA: 51.0
Ethylmalonyl-CoA: 48.9
CHC-CoA: 43.7
fatty_acid: 36.9
Acetyl-CoA: 31.1
trans-1,2-CPDA: 30.3
Benzoyl-CoA: 25.6
3-Methylbutyryl-CoA: 15.4

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%; Propionyl-CoA: 54.2%

c00014_3300017.. - Region 1 - NRPS-like

Download region GenBank file

Location: 820 - 43,510 nt. (total: 42,691 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 819 - 43510:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_15

Search NORINE for peptide: strict or relaxed

AMP-binding (17..443): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: pFvlgGGpVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)