3300021540_2 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300021.. (original name was: 3300021540.a:Ga0214517_100023)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS	90,641	133,631

c00007_3300021.. (original name was: 3300021540.a:Ga0214517_100003)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	sactipeptide	35,094	55,246
Region 7.2	NRPS	204,385	251,614

c00009_3300021.. (original name was: 3300021540.a:Ga0214517_100002)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	lassopeptide	66,602	89,895

Region	Type	From	To	Similarity
Region 3.1	NRPS	90,641	133,631
Region 7.1	sactipeptide	35,094	55,246
Region 7.2	NRPS	204,385	251,614
Region 9.1	lassopeptide	66,602	89,895

No secondary metabolite regions were found in these records:: c00001_3300021.. (original name was: 3300021540.a:Ga0214517_100063); c00002_3300021.. (original name was: 3300021540.a:Ga0214517_100009); c00004_3300021.. (original name was: 3300021540.a:Ga0214517_100001); c00005_3300021.. (original name was: 3300021540.a:Ga0214517_100400); c00006_3300021.. (original name was: 3300021540.a:Ga0214517_100042); c00008_3300021.. (original name was: 3300021540.a:Ga0214517_100013)

Compact view

c00003_3300021.. - Region 1 - NRPS

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Location: 90,641 - 133,631 nt. (total: 42,991 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 90640 - 133631:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg3_96

Search NORINE for peptide: strict or relaxed

AMP-binding (12..372): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: vAvFfGIfaK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 50% (weak)

c00007_3300021.. - Region 1 - sactipeptide

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Location: 35,094 - 55,246 nt. (total: 20,153 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or TIGR03973)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Sactipeptides

Sactipeptide predictions

Sactipeptides - No core peptides found.

Sactipeptides

Core peptide predictions

No core peptides found.

c00007_3300021.. - Region 2 - NRPS

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Location: 204,385 - 251,614 nt. (total: 47,230 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 204384 - 251614:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg7_184

Search NORINE for peptide: strict or relaxed

AMP-binding (434..820): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAmLfGAVFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

AMP-binding (1435..1831): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQfdGgVtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)

c00009_3300021.. - Region 1 - lassopeptide

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Location: 66,602 - 89,895 nt. (total: 23,294 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lasso peptides

Lasso peptide predictions

Lassopeptide(s) for ctg9_82 - Putative Class II

ctg9_82 leader / core peptide / cleaved tail: MFEIMETFELPTLFVVGQVKELTG - SSGKDNDDGFGYDNCA

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

ctg9_82 - Class II: Cleavage pHMM score: 1.90; RODEO score: 21; Monoisotopic mass: 1645.6 Da; Molecular weight: 1646.6 Da; Number of bridges: 0