3300021541_2 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300021.. (original name was: 3300021541.a:Ga0214529_10010)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	NRPS	91,283	134,273

c00006_3300021.. (original name was: 3300021541.a:Ga0214529_10003)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	sactipeptide	35,736	55,888
Region 6.2	NRPS	205,027	252,256

c00007_3300021.. (original name was: 3300021541.a:Ga0214529_10002)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	lassopeptide	66,602	89,895

Region	Type	From	To	Similarity
Region 3.1	NRPS	91,283	134,273
Region 6.1	sactipeptide	35,736	55,888
Region 6.2	NRPS	205,027	252,256
Region 7.1	lassopeptide	66,602	89,895

No secondary metabolite regions were found in these records:: c00001_3300021.. (original name was: 3300021541.a:Ga0214529_10020); c00002_3300021.. (original name was: 3300021541.a:Ga0214529_10015); c00004_3300021.. (original name was: 3300021541.a:Ga0214529_10007); c00005_3300021.. (original name was: 3300021541.a:Ga0214529_10006); c00008_3300021.. (original name was: 3300021541.a:Ga0214529_10045); c00009_3300021.. (original name was: 3300021541.a:Ga0214529_10001)

Compact view

c00003_3300021.. - Region 1 - NRPS

Download region GenBank file

Location: 91,283 - 134,273 nt. (total: 42,991 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 91282 - 134273:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg3_96

Search NORINE for peptide: strict or relaxed

AMP-binding (12..372): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: vAvFfGIfaK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 50% (weak)

c00006_3300021.. - Region 1 - sactipeptide

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Location: 35,736 - 55,888 nt. (total: 20,153 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or TIGR03973)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Sactipeptides

Sactipeptide predictions

Sactipeptides - No core peptides found.

Sactipeptides

Core peptide predictions

No core peptides found.

c00006_3300021.. - Region 2 - NRPS

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Location: 205,027 - 252,256 nt. (total: 47,230 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 205026 - 252256:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_184

Search NORINE for peptide: strict or relaxed

AMP-binding (434..820): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAmLfGAVFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

AMP-binding (1435..1831): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQfdGgVtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)

c00007_3300021.. - Region 1 - lassopeptide

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Location: 66,602 - 89,895 nt. (total: 23,294 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Lasso peptides

Lasso peptide predictions

Lassopeptide(s) for ctg7_82 - Putative Class II

ctg7_82 leader / core peptide / cleaved tail: MFEIMETFELPTLFVVGQVKELTG - SSGKDNDDGFGYDNCA

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

ctg7_82 - Class II: Cleavage pHMM score: 1.90; RODEO score: 21; Monoisotopic mass: 1645.6 Da; Molecular weight: 1646.6 Da; Number of bridges: 0