3300022177_6 - 3 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00006_3300022.. (original name was: 3300022177.a:Ga0228536_1000005)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS,T1PKS	482,507	555,977

c00013_3300022.. (original name was: 3300022177.a:Ga0228536_1000178)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	NRPS,T1PKS	46,754	113,187

c00014_3300022.. (original name was: 3300022177.a:Ga0228536_1000019)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	thiopeptide,LAP,bacteriocin	279,717	312,199

Region	Type	From	To	Similarity
Region 6.1	NRPS,T1PKS	482,507	555,977
Region 13.1	NRPS,T1PKS	46,754	113,187
Region 14.1	thiopeptide,LAP,bacteriocin	279,717	312,199

No secondary metabolite regions were found in these records:: c00001_3300022.. (original name was: 3300022177.a:Ga0228536_1001264); c00002_3300022.. (original name was: 3300022177.a:Ga0228536_1000230); c00003_3300022.. (original name was: 3300022177.a:Ga0228536_1000182); c00004_3300022.. (original name was: 3300022177.a:Ga0228536_1000061); c00005_3300022.. (original name was: 3300022177.a:Ga0228536_1000166); c00007_3300022.. (original name was: 3300022177.a:Ga0228536_1000781); c00008_3300022.. (original name was: 3300022177.a:Ga0228536_1000045); c00009_3300022.. (original name was: 3300022177.a:Ga0228536_1000660); c00010_3300022.. (original name was: 3300022177.a:Ga0228536_1001605); c00011_3300022.. (original name was: 3300022177.a:Ga0228536_1000113); c00012_3300022.. (original name was: 3300022177.a:Ga0228536_1000312); c00015_3300022.. (original name was: 3300022177.a:Ga0228536_1000112); c00016_3300022.. (original name was: 3300022177.a:Ga0228536_1000272); c00017_3300022.. (original name was: 3300022177.a:Ga0228536_1005799); c00018_3300022.. (original name was: 3300022177.a:Ga0228536_1001039); c00019_3300022.. (original name was: 3300022177.a:Ga0228536_1001064); c00020_3300022.. (original name was: 3300022177.a:Ga0228536_1000012); c00021_3300022.. (original name was: 3300022177.a:Ga0228536_1000268); c00022_3300022.. (original name was: 3300022177.a:Ga0228536_1001325); c00023_3300022.. (original name was: 3300022177.a:Ga0228536_1002675); c00024_3300022.. (original name was: 3300022177.a:Ga0228536_1000039); c00025_3300022.. (original name was: 3300022177.a:Ga0228536_1000815); c00026_3300022.. (original name was: 3300022177.a:Ga0228536_1002443); c00027_3300022.. (original name was: 3300022177.a:Ga0228536_1001179)

Compact view

c00006_3300022.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 482,507 - 555,977 nt. (total: 73,471 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 482506 - 555977:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (tyr) + (ser) + (D-asn) + (X - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_466

Search NORINE for peptide: strict or relaxed

AMP-binding (875..1236): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: agQDsGasEK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 50% (weak)

ctg6_468

Search NORINE for peptide: strict or relaxed

AMP-binding (265..662): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DGAaTaEVtK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 70% (weak)

ctg6_469

Search NORINE for peptide: strict or relaxed

AMP-binding (480..892): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg6_472

Search NORINE for peptide: strict or relaxed

CAL_domain (45..485): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 120.6
NH2: 51.7
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1137..1533): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg6_473

Search NORINE for peptide: strict or relaxed

AMP-binding (458..857): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvtncAlVYK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 50% (weak)

PKS_AT (1587..1888): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 143.1
inactive: 81.7
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 56.4
Propionyl-CoA: 35.1
trans-1,2-CPDA: 22.8
Ethylmalonyl-CoA: 19.9
Benzoyl-CoA: 14.0
2-Methylbutyryl-CoA: 11.7
fatty_acid: 7.6
CHC-CoA: 7.5
Isobutyryl-CoA: 5.4
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

c00013_3300022.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 46,754 - 113,187 nt. (total: 66,434 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 46753 - 113187:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal) + (val - ser - ohmal - val) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg13_58

Search NORINE for peptide: strict or relaxed

AMP-binding (432..715): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DpQDiGaVaK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)

ctg13_59

Search NORINE for peptide: strict or relaxed

AMP-binding (1076..1471): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

AMP-binding (2132..2529): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (4569..4962): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

ctg13_60

Search NORINE for peptide: strict or relaxed

AMP-binding (434..834): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DpqNVGMVdK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

PKS_AT (1563..1847): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 144.5
inactive: 78.2
Methoxymalonyl-CoA: 59.2
Methylmalonyl-CoA: 39.4
Propionyl-CoA: 25.4
Isobutyryl-CoA: 19.5
2-Methylbutyryl-CoA: 16.6
CHC-CoA: 15.3
Ethylmalonyl-CoA: 13.5
Benzoyl-CoA: 9.9
fatty_acid: 6.4
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

c00014_3300022.. - Region 1 - LAP,bacteriocin,thiopeptide

Download region GenBank file

Location: 279,717 - 312,199 nt. (total: 32,483 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03882) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)
LAP: ((goadsporin_like or PF00881 or TIGR03605) and (YcaO or TIGR03882))
bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

Select a gene to view the details available for it

Thiopeptides

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.