3300022821_3 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00009_3300022.. (original name was: 3300022821.a:Ga0222673_1000136)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	T3PKS	1	32,072

c00010_3300022.. (original name was: 3300022821.a:Ga0222673_1000007)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	NRPS,T1PKS	1	69,583

c00017_3300022.. (original name was: 3300022821.a:Ga0222673_1000006)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	terpene	56,687	77,652

c00087_3300022.. (original name was: 3300022821.a:Ga0222673_1000090)

Region	Type	From	To	Most similar known cluster		Similarity
Region 87.1	terpene	41,353	55,856

c00090_3300022.. (original name was: 3300022821.a:Ga0222673_1000305)

Region	Type	From	To	Most similar known cluster		Similarity
Region 90.1	hserlactone	7,682	25,533

Region	Type	From	To	Similarity
Region 9.1	T3PKS	1	32,072
Region 10.1	NRPS,T1PKS	1	69,583
Region 17.1	terpene	56,687	77,652
Region 87.1	terpene	41,353	55,856
Region 90.1	hserlactone	7,682	25,533

No secondary metabolite regions were found in these records:: c00001_3300022.. (original name was: 3300022821.a:Ga0222673_1004851); c00002_3300022.. (original name was: 3300022821.a:Ga0222673_1003891); c00003_3300022.. (original name was: 3300022821.a:Ga0222673_1003137); c00004_3300022.. (original name was: 3300022821.a:Ga0222673_1000186); c00005_3300022.. (original name was: 3300022821.a:Ga0222673_1000085); c00006_3300022.. (original name was: 3300022821.a:Ga0222673_1000096); c00007_3300022.. (original name was: 3300022821.a:Ga0222673_1000043); c00008_3300022.. (original name was: 3300022821.a:Ga0222673_1000269); c00011_3300022.. (original name was: 3300022821.a:Ga0222673_1000019); c00012_3300022.. (original name was: 3300022821.a:Ga0222673_1000016); c00013_3300022.. (original name was: 3300022821.a:Ga0222673_1000065); c00014_3300022.. (original name was: 3300022821.a:Ga0222673_1000013); c00015_3300022.. (original name was: 3300022821.a:Ga0222673_1004297); c00016_3300022.. (original name was: 3300022821.a:Ga0222673_1000123); c00018_3300022.. (original name was: 3300022821.a:Ga0222673_1003765); c00019_3300022.. (original name was: 3300022821.a:Ga0222673_1003748); c00020_3300022.. (original name was: 3300022821.a:Ga0222673_1003535); c00021_3300022.. (original name was: 3300022821.a:Ga0222673_1000244); c00022_3300022.. (original name was: 3300022821.a:Ga0222673_1001387); c00023_3300022.. (original name was: 3300022821.a:Ga0222673_1000559); c00024_3300022.. (original name was: 3300022821.a:Ga0222673_1000135); c00025_3300022.. (original name was: 3300022821.a:Ga0222673_1000152); c00026_3300022.. (original name was: 3300022821.a:Ga0222673_1000079); c00027_3300022.. (original name was: 3300022821.a:Ga0222673_1000036); c00028_3300022.. (original name was: 3300022821.a:Ga0222673_1000063); c00029_3300022.. (original name was: 3300022821.a:Ga0222673_1000026); c00030_3300022.. (original name was: 3300022821.a:Ga0222673_1001519); c00031_3300022.. (original name was: 3300022821.a:Ga0222673_1000098); c00032_3300022.. (original name was: 3300022821.a:Ga0222673_1000041); c00033_3300022.. (original name was: 3300022821.a:Ga0222673_1000556); c00034_3300022.. (original name was: 3300022821.a:Ga0222673_1000059); c00035_3300022.. (original name was: 3300022821.a:Ga0222673_1004165); c00036_3300022.. (original name was: 3300022821.a:Ga0222673_1000150); c00037_3300022.. (original name was: 3300022821.a:Ga0222673_1001643); c00038_3300022.. (original name was: 3300022821.a:Ga0222673_1000248); c00039_3300022.. (original name was: 3300022821.a:Ga0222673_1000273); c00040_3300022.. (original name was: 3300022821.a:Ga0222673_1000514); c00041_3300022.. (original name was: 3300022821.a:Ga0222673_1000156); c00042_3300022.. (original name was: 3300022821.a:Ga0222673_1000015); c00043_3300022.. (original name was: 3300022821.a:Ga0222673_1000340); c00044_3300022.. (original name was: 3300022821.a:Ga0222673_1000368); c00045_3300022.. (original name was: 3300022821.a:Ga0222673_1000115); c00046_3300022.. (original name was: 3300022821.a:Ga0222673_1000592); c00047_3300022.. (original name was: 3300022821.a:Ga0222673_1000868); c00048_3300022.. (original name was: 3300022821.a:Ga0222673_1000153); c00049_3300022.. (original name was: 3300022821.a:Ga0222673_1000031); c00050_3300022.. (original name was: 3300022821.a:Ga0222673_1001942); c00051_3300022.. (original name was: 3300022821.a:Ga0222673_1000074); c00052_3300022.. (original name was: 3300022821.a:Ga0222673_1001295); c00053_3300022.. (original name was: 3300022821.a:Ga0222673_1003211); c00054_3300022.. (original name was: 3300022821.a:Ga0222673_1001884); c00055_3300022.. (original name was: 3300022821.a:Ga0222673_1002138); c00056_3300022.. (original name was: 3300022821.a:Ga0222673_1000722); c00057_3300022.. (original name was: 3300022821.a:Ga0222673_1002688); c00058_3300022.. (original name was: 3300022821.a:Ga0222673_1000373); c00059_3300022.. (original name was: 3300022821.a:Ga0222673_1000349); c00060_3300022.. (original name was: 3300022821.a:Ga0222673_1001343); c00061_3300022.. (original name was: 3300022821.a:Ga0222673_1000436); c00062_3300022.. (original name was: 3300022821.a:Ga0222673_1000449); c00063_3300022.. (original name was: 3300022821.a:Ga0222673_1000459); c00064_3300022.. (original name was: 3300022821.a:Ga0222673_1000553); c00065_3300022.. (original name was: 3300022821.a:Ga0222673_1000428); c00066_3300022.. (original name was: 3300022821.a:Ga0222673_1001280); c00067_3300022.. (original name was: 3300022821.a:Ga0222673_1000789); c00068_3300022.. (original name was: 3300022821.a:Ga0222673_1000930); c00069_3300022.. (original name was: 3300022821.a:Ga0222673_1000097); c00070_3300022.. (original name was: 3300022821.a:Ga0222673_1000277); c00071_3300022.. (original name was: 3300022821.a:Ga0222673_1001089); c00072_3300022.. (original name was: 3300022821.a:Ga0222673_1001316); c00073_3300022.. (original name was: 3300022821.a:Ga0222673_1001346); c00074_3300022.. (original name was: 3300022821.a:Ga0222673_1002709); c00075_3300022.. (original name was: 3300022821.a:Ga0222673_1002880); c00076_3300022.. (original name was: 3300022821.a:Ga0222673_1000578); c00077_3300022.. (original name was: 3300022821.a:Ga0222673_1000021); c00078_3300022.. (original name was: 3300022821.a:Ga0222673_1002413); c00079_3300022.. (original name was: 3300022821.a:Ga0222673_1002602); c00080_3300022.. (original name was: 3300022821.a:Ga0222673_1000054); c00081_3300022.. (original name was: 3300022821.a:Ga0222673_1000081); c00082_3300022.. (original name was: 3300022821.a:Ga0222673_1000104); c00083_3300022.. (original name was: 3300022821.a:Ga0222673_1000144); c00084_3300022.. (original name was: 3300022821.a:Ga0222673_1000251); c00085_3300022.. (original name was: 3300022821.a:Ga0222673_1000261); c00086_3300022.. (original name was: 3300022821.a:Ga0222673_1002822); c00088_3300022.. (original name was: 3300022821.a:Ga0222673_1000274); c00089_3300022.. (original name was: 3300022821.a:Ga0222673_1000297); c00091_3300022.. (original name was: 3300022821.a:Ga0222673_1000411); c00092_3300022.. (original name was: 3300022821.a:Ga0222673_1000047); c00093_3300022.. (original name was: 3300022821.a:Ga0222673_1000711); c00094_3300022.. (original name was: 3300022821.a:Ga0222673_1000265); c00095_3300022.. (original name was: 3300022821.a:Ga0222673_1001034); c00096_3300022.. (original name was: 3300022821.a:Ga0222673_1001594); c00097_3300022.. (original name was: 3300022821.a:Ga0222673_1003018); c00098_3300022.. (original name was: 3300022821.a:Ga0222673_1001730); c00099_3300022.. (original name was: 3300022821.a:Ga0222673_1001797); c00100_3300022.. (original name was: 3300022821.a:Ga0222673_1000902); c00101_3300022.. (original name was: 3300022821.a:Ga0222673_1002334)

Compact view

c00009_3300022.. - Region 1 - T3PKS

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Location: 1 - 32,072 nt. (total: 32,072 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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c00010_3300022.. - Region 1 - NRPS,T1PKS

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Location: 1 - 69,583 nt. (total: 69,583 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 69583:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (mal) + (ccmal) + (asn) + (thr - gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_7

Search NORINE for peptide: strict or relaxed

AMP-binding (547..979): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2011..2425): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMvyK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 80% (moderate)

ctg10_10

Search NORINE for peptide: strict or relaxed

AMP-binding (1160..1575): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg10_16

PKS_AT (897..1213): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; 2-Rhyd-Malonyl-CoA: 66.7%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 100.9
inactive: 78.7
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 38.2
Isobutyryl-CoA: 23.5
Benzoyl-CoA: 18.4
Propionyl-CoA: 11.1
3-Methylbutyryl-CoA: 10.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg10_17

PKS_AT (565..871): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.0
inactive: 128.6
Methoxymalonyl-CoA: 73.5
Methylmalonyl-CoA: 63.9
Propionyl-CoA: 45.1
Isobutyryl-CoA: 29.4
Ethylmalonyl-CoA: 29.3
CHC-CoA: 28.6
2-Methylbutyryl-CoA: 25.4
trans-1,2-CPDA: 21.3
Benzoyl-CoA: 15.7
Acetyl-CoA: 15.7
3-Methylbutyryl-CoA: 12.8
fatty_acid: 0.0

ctg10_18

PKS_AT (581..867): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.4
inactive: 117.2
Methoxymalonyl-CoA: 66.1
Methylmalonyl-CoA: 64.9
Acetyl-CoA: 33.2
Propionyl-CoA: 30.4
Ethylmalonyl-CoA: 28.6
Isobutyryl-CoA: 25.3
Benzoyl-CoA: 20.5
trans-1,2-CPDA: 18.0
2-Methylbutyryl-CoA: 18.0
CHC-CoA: 16.6
3-Methylbutyryl-CoA: 16.6
fatty_acid: 0.0

ctg10_19

CAL_domain (161..473): pk

minowa: NH2

Prediction, score:

NH2: 76.2
Acetyl-CoA: 24.9
fatty_acid: 8.0
shikimic_acid: 0.0
AHBA: 0.0

PKS_AT (1207..1490): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 132.7
inactive: 88.3
Methoxymalonyl-CoA: 61.2
Methylmalonyl-CoA: 56.9
Isobutyryl-CoA: 42.9
Propionyl-CoA: 28.5
fatty_acid: 22.1
3-Methylbutyryl-CoA: 18.2
CHC-CoA: 17.9
Benzoyl-CoA: 17.7
Acetyl-CoA: 17.7
Ethylmalonyl-CoA: 15.5
trans-1,2-CPDA: 13.2
2-Methylbutyryl-CoA: 0.0

c00017_3300022.. - Region 1 - terpene

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Location: 56,687 - 77,652 nt. (total: 20,966 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

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c00087_3300022.. - Region 1 - terpene

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Location: 41,353 - 55,856 nt. (total: 14,504 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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c00090_3300022.. - Region 1 - hserlactone

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Location: 7,682 - 25,533 nt. (total: 17,852 nt) Show pHMM detection rules used

Region on contig edge.

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it