3300025261_12 - 8 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00002_3300025.. (original name was: 3300025261.a:Ga0209233_1000179)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	bacteriocin	132,291	140,518

c00004_3300025.. (original name was: 3300025261.a:Ga0209233_1000009)

Region	Type	From	To
Region 4.1	terpene	242,607	263,446
Region 4.2	NRPS,T1PKS	881,157	938,829
Region 4.3	NRPS	1,214,484	1,265,567

c00005_3300025.. (original name was: 3300025261.a:Ga0209233_1000002)

Region	Type	From	To
Region 5.1	bacteriocin	28,628	39,114
Region 5.2	terpene	106,375	129,505
Region 5.3	bacteriocin	1,950,896	1,961,738
Region 5.4	arylpolyene	2,110,050	2,151,249

Region	Type	From	To	Similarity
Region 2.1	bacteriocin	132,291	140,518
Region 4.1	terpene	242,607	263,446
Region 4.2	NRPS,T1PKS	881,157	938,829
Region 4.3	NRPS	1,214,484	1,265,567
Region 5.1	bacteriocin	28,628	39,114
Region 5.2	terpene	106,375	129,505
Region 5.3	bacteriocin	1,950,896	1,961,738
Region 5.4	arylpolyene	2,110,050	2,151,249

No secondary metabolite regions were found in these records:: c00001_3300025.. (original name was: 3300025261.a:Ga0209233_1000040); c00003_3300025.. (original name was: 3300025261.a:Ga0209233_1000100); c00006_3300025.. (original name was: 3300025261.a:Ga0209233_1000136)

Compact view

c00002_3300025.. - Region 1 - bacteriocin

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Location: 132,291 - 140,518 nt. (total: 8,228 nt) Show pHMM detection rules used

Region on contig edge.

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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c00004_3300025.. - Region 1 - terpene

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Location: 242,607 - 263,446 nt. (total: 20,840 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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other genes

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c00004_3300025.. - Region 2 - NRPS,T1PKS

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Location: 881,157 - 938,829 nt. (total: 57,673 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 881156 - 938829:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - mal) + (gly - gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_847

Search NORINE for peptide: strict or relaxed

AMP-binding (117..314): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: Drp-iAtLIK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 50% (weak)

ctg4_851

Search NORINE for peptide: strict or relaxed

AMP-binding (491..888): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQiGaVWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 80% (moderate)

AMP-binding (1549..1944): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGmVWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 90% (moderate)

ctg4_852

Search NORINE for peptide: strict or relaxed

AMP-binding (215..608): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 100% (strong)

PKS_AT (1328..1617): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.7
inactive: 82.2
Methylmalonyl-CoA: 63.7
Methoxymalonyl-CoA: 60.8
Propionyl-CoA: 46.8
Acetyl-CoA: 29.9
Isobutyryl-CoA: 26.5
Benzoyl-CoA: 24.9
CHC-CoA: 24.2
Ethylmalonyl-CoA: 23.5
2-Methylbutyryl-CoA: 23.2
fatty_acid: 18.0
trans-1,2-CPDA: 15.6
3-Methylbutyryl-CoA: 10.3

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

c00004_3300025.. - Region 3 - NRPS

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Location: 1,214,484 - 1,265,567 nt. (total: 51,084 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 1214483 - 1265567:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (asp) + (val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_1117

Search NORINE for peptide: strict or relaxed

AMP-binding (473..875): X

NRPSPredictor2: tyr, bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvSviGAICK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)

ctg4_1118

Search NORINE for peptide: strict or relaxed

AMP-binding (496..899): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVaHVaK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 80% (moderate)

ctg4_1120

Search NORINE for peptide: strict or relaxed

AMP-binding (36..428): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DgFllGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 70% (weak)

c00005_3300025.. - Region 1 - bacteriocin

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Location: 28,628 - 39,114 nt. (total: 10,487 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

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c00005_3300025.. - Region 2 - terpene

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Location: 106,375 - 129,505 nt. (total: 23,131 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

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other genes

resistance

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Gene details

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c00005_3300025.. - Region 3 - bacteriocin

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Location: 1,950,896 - 1,961,738 nt. (total: 10,843 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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c00005_3300025.. - Region 4 - arylpolyene

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Location: 2,110,050 - 2,151,249 nt. (total: 41,200 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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