3300025297_10 - 11 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00001_3300025.. (original name was: 3300025297.a:Ga0209758_1000027)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	NRPS,T1PKS	30,144	98,489
Region 1.2	resorcinol,arylpolyene	447,291	493,223

c00002_3300025.. (original name was: 3300025297.a:Ga0209758_1000067)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	NRPS	96,829	164,747

c00007_3300025.. (original name was: 3300025297.a:Ga0209758_1000180)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	bacteriocin	61,584	72,366

c00013_3300025.. (original name was: 3300025297.a:Ga0209758_1000025)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	resorcinol	192,295	234,211

c00020_3300025.. (original name was: 3300025297.a:Ga0209758_1000042)

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	arylpolyene	137,677	178,879

c00025_3300025.. (original name was: 3300025297.a:Ga0209758_1000064)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	terpene	132,815	154,601

c00030_3300025.. (original name was: 3300025297.a:Ga0209758_1000496)

Region	Type	From	To	Most similar known cluster		Similarity
Region 30.1	NRPS	27,393	64,085

c00032_3300025.. (original name was: 3300025297.a:Ga0209758_1000330)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	NRPS	63,399	89,233

c00037_3300025.. (original name was: 3300025297.a:Ga0209758_1000037)

Region	Type	From	To	Most similar known cluster		Similarity
Region 37.1	hserlactone	112,498	133,094

c00038_3300025.. (original name was: 3300025297.a:Ga0209758_1000044)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	NRPS,T1PKS	93,956	146,317

Region	Type	From	To	Similarity
Region 1.1	NRPS,T1PKS	30,144	98,489
Region 1.2	resorcinol,arylpolyene	447,291	493,223
Region 2.1	NRPS	96,829	164,747
Region 7.1	bacteriocin	61,584	72,366
Region 13.1	resorcinol	192,295	234,211
Region 20.1	arylpolyene	137,677	178,879
Region 25.1	terpene	132,815	154,601
Region 30.1	NRPS	27,393	64,085
Region 32.1	NRPS	63,399	89,233
Region 37.1	hserlactone	112,498	133,094
Region 38.1	NRPS,T1PKS	93,956	146,317

No secondary metabolite regions were found in these records:: c00003_3300025.. (original name was: 3300025297.a:Ga0209758_1000909); c00004_3300025.. (original name was: 3300025297.a:Ga0209758_1000664); c00005_3300025.. (original name was: 3300025297.a:Ga0209758_1000127); c00006_3300025.. (original name was: 3300025297.a:Ga0209758_1000691); c00008_3300025.. (original name was: 3300025297.a:Ga0209758_1001355); c00009_3300025.. (original name was: 3300025297.a:Ga0209758_1000039); c00010_3300025.. (original name was: 3300025297.a:Ga0209758_1000184); c00011_3300025.. (original name was: 3300025297.a:Ga0209758_1000199); c00012_3300025.. (original name was: 3300025297.a:Ga0209758_1000107); c00014_3300025.. (original name was: 3300025297.a:Ga0209758_1000442); c00015_3300025.. (original name was: 3300025297.a:Ga0209758_1002886); c00016_3300025.. (original name was: 3300025297.a:Ga0209758_1008150); c00017_3300025.. (original name was: 3300025297.a:Ga0209758_1004533); c00018_3300025.. (original name was: 3300025297.a:Ga0209758_1008763); c00019_3300025.. (original name was: 3300025297.a:Ga0209758_1000529); c00021_3300025.. (original name was: 3300025297.a:Ga0209758_1000135); c00022_3300025.. (original name was: 3300025297.a:Ga0209758_1003331); c00023_3300025.. (original name was: 3300025297.a:Ga0209758_1000510); c00024_3300025.. (original name was: 3300025297.a:Ga0209758_1000134); c00026_3300025.. (original name was: 3300025297.a:Ga0209758_1004044); c00027_3300025.. (original name was: 3300025297.a:Ga0209758_1000049); c00028_3300025.. (original name was: 3300025297.a:Ga0209758_1000754); c00029_3300025.. (original name was: 3300025297.a:Ga0209758_1011588); c00031_3300025.. (original name was: 3300025297.a:Ga0209758_1000050); c00033_3300025.. (original name was: 3300025297.a:Ga0209758_1000142); c00034_3300025.. (original name was: 3300025297.a:Ga0209758_1000034); c00035_3300025.. (original name was: 3300025297.a:Ga0209758_1000222); c00036_3300025.. (original name was: 3300025297.a:Ga0209758_1000833); c00039_3300025.. (original name was: 3300025297.a:Ga0209758_1004046); c00040_3300025.. (original name was: 3300025297.a:Ga0209758_1000297); c00041_3300025.. (original name was: 3300025297.a:Ga0209758_1000092); c00042_3300025.. (original name was: 3300025297.a:Ga0209758_1000891); c00043_3300025.. (original name was: 3300025297.a:Ga0209758_1000132); c00044_3300025.. (original name was: 3300025297.a:Ga0209758_1001159)

Compact view

c00001_3300025.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 30,144 - 98,489 nt. (total: 68,346 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 30143 - 98489:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal) + (ser) + (asp) + (thr - D-X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg1_52

Search NORINE for peptide: strict or relaxed

CAL_domain (49..487): pk

minowa: NH2

Prediction, score:

NH2: 184.2
Acetyl-CoA: 44.5
fatty_acid: 6.9
AHBA: 5.4
shikimic_acid: 0.0

AMP-binding (1159..1555): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvWNIGliHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

ctg1_53

PKS_AT (544..830): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.8
inactive: 89.4
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 62.3
Propionyl-CoA: 47.3
Ethylmalonyl-CoA: 46.5
2-Methylbutyryl-CoA: 28.1
Isobutyryl-CoA: 26.9
CHC-CoA: 23.3
Acetyl-CoA: 18.4
fatty_acid: 18.2
Benzoyl-CoA: 17.2
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0

ctg1_54

Search NORINE for peptide: strict or relaxed

AMP-binding (517..915): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg1_55

Search NORINE for peptide: strict or relaxed

AMP-binding (442..833): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg1_56

Search NORINE for peptide: strict or relaxed

AMP-binding (490..893): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1584..1982): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvWNIGliHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

c00001_3300025.. - Region 2 - arylpolyene,resorcinol

Download region GenBank file

Location: 447,291 - 493,223 nt. (total: 45,933 nt) Show pHMM detection rules used

resorcinol: DarB
arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00002_3300025.. - Region 1 - NRPS

Download region GenBank file

Location: 96,829 - 164,747 nt. (total: 67,919 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 96828 - 164747:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr - pro - pro - X - X) + (ser) + (pro - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_111

Search NORINE for peptide: strict or relaxed

AMP-binding (577..986): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1643..2044): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMveLGMVDK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 80% (moderate)

ctg2_112

Search NORINE for peptide: strict or relaxed

AMP-binding (576..976): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg2_113

Search NORINE for peptide: strict or relaxed

AMP-binding (454..865): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1560..1964): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (2638..3041): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (3693..4096): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVenVGAInK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 70% (weak)

AMP-binding (4755..5157): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMveLGMVDK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 80% (moderate)

c00007_3300025.. - Region 1 - bacteriocin

Download region GenBank file

Location: 61,584 - 72,366 nt. (total: 10,783 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00013_3300025.. - Region 1 - resorcinol

Download region GenBank file

Location: 192,295 - 234,211 nt. (total: 41,917 nt) Show pHMM detection rules used

resorcinol: DarB

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00020_3300025.. - Region 1 - arylpolyene

Download region GenBank file

Location: 137,677 - 178,879 nt. (total: 41,203 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00025_3300025.. - Region 1 - terpene

Download region GenBank file

Location: 132,815 - 154,601 nt. (total: 21,787 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00030_3300025.. - Region 1 - NRPS

Download region GenBank file

Location: 27,393 - 64,085 nt. (total: 36,693 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 27392 - 64085:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg30_49

Search NORINE for peptide: strict or relaxed

AMP-binding (467..871): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (1537..1930): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAmlvGAICK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)

c00032_3300025.. - Region 1 - NRPS

Download region GenBank file

Location: 63,399 - 89,233 nt. (total: 25,835 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 63398 - 89233:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg32_58

Search NORINE for peptide: strict or relaxed

AMP-binding (462..870): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: iDWVsgLdaK
Nearest Stachelhaus code:: ala-b
Stachelhaus code match:: 50% (weak)

c00037_3300025.. - Region 1 - hserlactone

Download region GenBank file

Location: 112,498 - 133,094 nt. (total: 20,597 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00038_3300025.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 93,956 - 146,317 nt. (total: 52,362 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 93955 - 146317:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg38_111

Search NORINE for peptide: strict or relaxed

AMP-binding (486..883): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMpqwGGVyK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 60% (weak)

ctg38_112

Search NORINE for peptide: strict or relaxed

AMP-binding (230..625): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DALWfGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

PKS_AT (1357..1639): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 87.5%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 140.9
inactive: 68.7
Methoxymalonyl-CoA: 61.0
Methylmalonyl-CoA: 53.2
Propionyl-CoA: 36.4
Isobutyryl-CoA: 25.3
2-Methylbutyryl-CoA: 23.0
Ethylmalonyl-CoA: 21.8
fatty_acid: 20.0
Benzoyl-CoA: 16.8
CHC-CoA: 16.0
Acetyl-CoA: 12.8
trans-1,2-CPDA: 7.6
3-Methylbutyryl-CoA: 0.0