3300025304_10 - 14 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00004_3300025.. (original name was: 3300025304.a:Ga0209257_1000004)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	resorcinol,arylpolyene	1,458,607	1,505,092

c00005_3300025.. (original name was: 3300025304.a:Ga0209257_1000070)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	lanthipeptide	268,910	291,084

c00007_3300025.. (original name was: 3300025304.a:Ga0209257_1000064)

Region	Type	From	To
Region 7.1	terpene	8,919	26,981
Region 7.2	terpene	224,903	245,877
Region 7.3	NRPS	301,926	356,803

c00008_3300025.. (original name was: 3300025304.a:Ga0209257_1000089)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	NRPS	1	47,261

c00009_3300025.. (original name was: 3300025304.a:Ga0209257_1000008)

Region	Type	From	To
Region 9.1	T3PKS	234	41,286
Region 9.2	terpene	120,077	140,470
Region 9.3	lassopeptide	186,589	208,847
Region 9.4	terpene	463,236	484,201

c00010_3300025.. (original name was: 3300025304.a:Ga0209257_1000077)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	lanthipeptide	2,637	27,845

c00011_3300025.. (original name was: 3300025304.a:Ga0209257_1000071)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	NRPS,transAT-PKS	250,081	335,832

c00014_3300025.. (original name was: 3300025304.a:Ga0209257_1000013)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	terpene	407,613	428,440
Region 14.2	NRPS,lanthipeptide	1,008,945	1,047,305

Region	Type	From	To	Similarity
Region 4.1	resorcinol,arylpolyene	1,458,607	1,505,092
Region 5.1	lanthipeptide	268,910	291,084
Region 7.1	terpene	8,919	26,981
Region 7.2	terpene	224,903	245,877
Region 7.3	NRPS	301,926	356,803
Region 8.1	NRPS	1	47,261
Region 9.1	T3PKS	234	41,286
Region 9.2	terpene	120,077	140,470
Region 9.3	lassopeptide	186,589	208,847
Region 9.4	terpene	463,236	484,201
Region 10.1	lanthipeptide	2,637	27,845
Region 11.1	NRPS,transAT-PKS	250,081	335,832
Region 14.1	terpene	407,613	428,440
Region 14.2	NRPS,lanthipeptide	1,008,945	1,047,305

No secondary metabolite regions were found in these records:: c00001_3300025.. (original name was: 3300025304.a:Ga0209257_1000233); c00002_3300025.. (original name was: 3300025304.a:Ga0209257_1000770); c00003_3300025.. (original name was: 3300025304.a:Ga0209257_1000280); c00006_3300025.. (original name was: 3300025304.a:Ga0209257_1000511); c00012_3300025.. (original name was: 3300025304.a:Ga0209257_1000025); c00013_3300025.. (original name was: 3300025304.a:Ga0209257_1000959); c00015_3300025.. (original name was: 3300025304.a:Ga0209257_1000128)

Compact view

c00004_3300025.. - Region 1 - arylpolyene,resorcinol

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Location: 1,458,607 - 1,505,092 nt. (total: 46,486 nt) Show pHMM detection rules used

resorcinol: DarB
arylpolyene: (APE_KS1 or APE_KS2)

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c00005_3300025.. - Region 1 - lanthipeptide

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Location: 268,910 - 291,084 nt. (total: 22,175 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

c00007_3300025.. - Region 1 - terpene

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Location: 8,919 - 26,981 nt. (total: 18,063 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00007_3300025.. - Region 2 - terpene

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Location: 224,903 - 245,877 nt. (total: 20,975 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00007_3300025.. - Region 3 - NRPS

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Location: 301,926 - 356,803 nt. (total: 54,878 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 3, location 301925 - 356803:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-asp) + (tyr) + (D-X - ala-b) + (gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg7_243

Search NORINE for peptide: strict or relaxed

AMP-binding (458..829): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg7_254

Search NORINE for peptide: strict or relaxed

AMP-binding (545..923): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAEhIGeVtK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 70% (weak)

AMP-binding (2048..2441): ala-b

NRPSPredictor2: ala-b

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: VDWVtSiADK
Nearest Stachelhaus code:: ala-b
Stachelhaus code match:: 80% (moderate)

ctg7_255

Search NORINE for peptide: strict or relaxed

AMP-binding (539..932): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg7_256

Search NORINE for peptide: strict or relaxed

AMP-binding (591..991): asp

NRPSPredictor2: asp

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKiGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 90% (moderate)

c00008_3300025.. - Region 1 - NRPS

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Location: 1 - 47,261 nt. (total: 47,261 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 47261:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (tyr - D-asn - D-ser - D-tyr - X) + (asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_1

Search NORINE for peptide: strict or relaxed

AMP-binding (409..799): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg8_3

Search NORINE for peptide: strict or relaxed

AMP-binding (252..642): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

AMP-binding (1291..1688): asn

NRPSPredictor2: asn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGhVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)

AMP-binding (2812..3215): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHlSLVeK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 80% (moderate)

AMP-binding (4332..4728): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

A-OX (5867..6631): X

c00009_3300025.. - Region 1 - T3PKS

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Location: 234 - 41,286 nt. (total: 41,053 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

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c00009_3300025.. - Region 2 - terpene

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Location: 120,077 - 140,470 nt. (total: 20,394 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00009_3300025.. - Region 3 - lassopeptide

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Location: 186,589 - 208,847 nt. (total: 22,259 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

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Lasso peptides

Lasso peptide predictions

Lassopeptides - No core peptides found.

Lasso peptides

Core peptide predictions

No core peptides found.

c00009_3300025.. - Region 4 - terpene

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Location: 463,236 - 484,201 nt. (total: 20,966 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00010_3300025.. - Region 1 - lanthipeptide

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Location: 2,637 - 27,845 nt. (total: 25,209 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

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Lanthipeptides

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

Core peptide predictions

No core peptides found.

c00011_3300025.. - Region 1 - NRPS,transAT-PKS

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Location: 250,081 - 335,832 nt. (total: 85,752 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
transAT-PKS: (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 250080 - 335832:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (X) + (asp - orn) + (asp) + (ohmal) + (asp) + (mal - orn - ala) + (glu - leu - gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg11_196

Search NORINE for peptide: strict or relaxed

AMP-binding (546..928): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DAKhsGtVnK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 60% (weak)

AMP-binding (1591..1979): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (2636..3038): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVnK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

ctg11_197

Search NORINE for peptide: strict or relaxed

AMP-binding (2698..3104): orn

NRPSPredictor2: orn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

AMP-binding (3773..4147): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

ctg11_198

PKS_AT (6..287): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.6
Methoxymalonyl-CoA: 43.7
inactive: 42.4
Methylmalonyl-CoA: 42.1
Propionyl-CoA: 21.4
fatty_acid: 19.5
CHC-CoA: 17.4
2-Methylbutyryl-CoA: 10.6
Ethylmalonyl-CoA: 10.4
Acetyl-CoA: 8.9
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%

ctg11_199

Search NORINE for peptide: strict or relaxed

AMP-binding (249..650): asp

NRPSPredictor2: asp

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKfGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 90% (moderate)

ctg11_201

Search NORINE for peptide: strict or relaxed

AMP-binding (473..869): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg11_202

Search NORINE for peptide: strict or relaxed

AMP-binding (476..873): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKlGHIGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 90% (moderate)

AMP-binding (1930..2221): orn

NRPSPredictor2: orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVADiGcIeK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 70% (weak)

ctg11_204

Search NORINE for peptide: strict or relaxed

AMP-binding (12..434): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DArHLaLmaK
Nearest Stachelhaus code:: hpg
Stachelhaus code match:: 60% (weak)

ctg11_209

Search NORINE for peptide: strict or relaxed

AMP-binding (9..441): X

NRPSPredictor2: pro, pip

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DArHLsLmvK
Nearest Stachelhaus code:: hpg
Stachelhaus code match:: 60% (weak)

ctg11_210

Search NORINE for peptide: strict or relaxed

AMP-binding (9..427): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DArHLaLmaK
Nearest Stachelhaus code:: hpg
Stachelhaus code match:: 60% (weak)

c00014_3300025.. - Region 1 - terpene

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Location: 407,613 - 428,440 nt. (total: 20,828 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00014_3300025.. - Region 2 - NRPS,lanthipeptide

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Location: 1,008,945 - 1,047,305 nt. (total: 38,361 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

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NRPS/PKS domains

Lanthipeptides

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

NRPS/PKS products

NRPS/PKS monomers

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 2, location 1008944 - 1047305:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (lys - orn - gln - val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_892

Search NORINE for peptide: strict or relaxed

CAL_domain (36..482): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 143.3
NH2: 36.8
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1125..1518): lys

NRPSPredictor2: lys

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DtESIGtVCK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 80% (moderate)

AMP-binding (2178..2584): orn

NRPSPredictor2: orn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

AMP-binding (3243..3631): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGVVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

AMP-binding (4290..4671): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWlGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)