3300025522_1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300025.. (original name was: 3300025522.a:Ga0208371_1000026)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	terpene	1	14,403

c00025_3300025.. (original name was: 3300025522.a:Ga0208371_1000039)

Region	Type	From	To	Most similar known cluster		Similarity
Region 25.1	NRPS,T1PKS	89	112,488

Region	Type	From	To	Most similar known cluster	Similarity
Region 3.1	terpene	1	14,403
Region 25.1	NRPS,T1PKS	89	112,488

No secondary metabolite regions were found in these records:: c00001_3300025.. (original name was: 3300025522.a:Ga0208371_1001087); c00002_3300025.. (original name was: 3300025522.a:Ga0208371_1000100); c00004_3300025.. (original name was: 3300025522.a:Ga0208371_1000338); c00005_3300025.. (original name was: 3300025522.a:Ga0208371_1000075); c00006_3300025.. (original name was: 3300025522.a:Ga0208371_1000281); c00007_3300025.. (original name was: 3300025522.a:Ga0208371_1000037); c00008_3300025.. (original name was: 3300025522.a:Ga0208371_1000055); c00009_3300025.. (original name was: 3300025522.a:Ga0208371_1000470); c00010_3300025.. (original name was: 3300025522.a:Ga0208371_1000105); c00011_3300025.. (original name was: 3300025522.a:Ga0208371_1000056); c00012_3300025.. (original name was: 3300025522.a:Ga0208371_1000017); c00013_3300025.. (original name was: 3300025522.a:Ga0208371_1000067); c00014_3300025.. (original name was: 3300025522.a:Ga0208371_1000072); c00015_3300025.. (original name was: 3300025522.a:Ga0208371_1000025); c00016_3300025.. (original name was: 3300025522.a:Ga0208371_1000048); c00017_3300025.. (original name was: 3300025522.a:Ga0208371_1000066); c00018_3300025.. (original name was: 3300025522.a:Ga0208371_1000155); c00019_3300025.. (original name was: 3300025522.a:Ga0208371_1000108); c00020_3300025.. (original name was: 3300025522.a:Ga0208371_1000029); c00021_3300025.. (original name was: 3300025522.a:Ga0208371_1000440); c00022_3300025.. (original name was: 3300025522.a:Ga0208371_1000052); c00023_3300025.. (original name was: 3300025522.a:Ga0208371_1000062); c00024_3300025.. (original name was: 3300025522.a:Ga0208371_1000336); c00026_3300025.. (original name was: 3300025522.a:Ga0208371_1000274); c00027_3300025.. (original name was: 3300025522.a:Ga0208371_1000085); c00028_3300025.. (original name was: 3300025522.a:Ga0208371_1000550); c00029_3300025.. (original name was: 3300025522.a:Ga0208371_1000065); c00030_3300025.. (original name was: 3300025522.a:Ga0208371_1000306); c00031_3300025.. (original name was: 3300025522.a:Ga0208371_1000015); c00032_3300025.. (original name was: 3300025522.a:Ga0208371_1000112); c00033_3300025.. (original name was: 3300025522.a:Ga0208371_1000018); c00034_3300025.. (original name was: 3300025522.a:Ga0208371_1000309); c00035_3300025.. (original name was: 3300025522.a:Ga0208371_1000027); c00036_3300025.. (original name was: 3300025522.a:Ga0208371_1000007); c00037_3300025.. (original name was: 3300025522.a:Ga0208371_1000104); c00038_3300025.. (original name was: 3300025522.a:Ga0208371_1000068); c00039_3300025.. (original name was: 3300025522.a:Ga0208371_1000036); c00040_3300025.. (original name was: 3300025522.a:Ga0208371_1000030); c00041_3300025.. (original name was: 3300025522.a:Ga0208371_1000877); c00042_3300025.. (original name was: 3300025522.a:Ga0208371_1000031); c00043_3300025.. (original name was: 3300025522.a:Ga0208371_1000538); c00044_3300025.. (original name was: 3300025522.a:Ga0208371_1000053); c00045_3300025.. (original name was: 3300025522.a:Ga0208371_1000084); c00046_3300025.. (original name was: 3300025522.a:Ga0208371_1000012); c00047_3300025.. (original name was: 3300025522.a:Ga0208371_1000756); c00048_3300025.. (original name was: 3300025522.a:Ga0208371_1000035); c00049_3300025.. (original name was: 3300025522.a:Ga0208371_1000076); c00050_3300025.. (original name was: 3300025522.a:Ga0208371_1000270); c00051_3300025.. (original name was: 3300025522.a:Ga0208371_1000088); c00052_3300025.. (original name was: 3300025522.a:Ga0208371_1000042); c00053_3300025.. (original name was: 3300025522.a:Ga0208371_1000033); c00054_3300025.. (original name was: 3300025522.a:Ga0208371_1000040); c00055_3300025.. (original name was: 3300025522.a:Ga0208371_1000160); c00056_3300025.. (original name was: 3300025522.a:Ga0208371_1000063); c00057_3300025.. (original name was: 3300025522.a:Ga0208371_1000016); c00058_3300025.. (original name was: 3300025522.a:Ga0208371_1000855); c00059_3300025.. (original name was: 3300025522.a:Ga0208371_1000043); c00060_3300025.. (original name was: 3300025522.a:Ga0208371_1000140); c00061_3300025.. (original name was: 3300025522.a:Ga0208371_1000237); c00062_3300025.. (original name was: 3300025522.a:Ga0208371_1000049); c00063_3300025.. (original name was: 3300025522.a:Ga0208371_1000064)

Compact view

c00025_3300025.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 89 - 112,488 nt. (total: 112,400 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 88 - 112488:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pro) + (tyr) + (X - ohmal) + (X) + (ohmal) + (D-gln) + (D-arg) + (leu) + (pk) + (thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 88 - 100907:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pro) + (tyr) + (X - ohmal) + (X) + (ohmal) + (D-gln) + (D-arg) + (leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 80976 - 112488:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu) + (pk) + (thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 87523 - 112488:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg25_14

Search NORINE for peptide: strict or relaxed

AMP-binding (1112..1470): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

ctg25_17

Search NORINE for peptide: strict or relaxed

AMP-binding (283..666): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: tyr

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg25_23

Search NORINE for peptide: strict or relaxed

AMP-binding (2154..2578): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeMtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

PKS_AT (3307..3596): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 157.7
inactive: 93.8
Methoxymalonyl-CoA: 62.3
Methylmalonyl-CoA: 47.3
Propionyl-CoA: 28.4
Ethylmalonyl-CoA: 23.0
Isobutyryl-CoA: 20.1
fatty_acid: 12.3
Acetyl-CoA: 11.6
Benzoyl-CoA: 11.0
2-Methylbutyryl-CoA: 10.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

ctg25_24

Search NORINE for peptide: strict or relaxed

AMP-binding (549..955): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DlWNIGfVwK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

ctg25_29

PKS_AT (533..823): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 178.7
inactive: 97.0
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 60.6
Propionyl-CoA: 47.5
Ethylmalonyl-CoA: 29.0
2-Methylbutyryl-CoA: 23.9
CHC-CoA: 22.2
Isobutyryl-CoA: 20.6
trans-1,2-CPDA: 15.9
Acetyl-CoA: 14.1
Benzoyl-CoA: 11.4
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

ctg25_32

Search NORINE for peptide: strict or relaxed

AMP-binding (588..997): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDiGVVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

ctg25_37

Search NORINE for peptide: strict or relaxed

AMP-binding (473..877): arg

NRPSPredictor2: arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: glu, gln
Single AA prediction:: arg

Stachelhaus prediction details:

Stachelhaus sequence:: DgqgiGlVNK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 50% (weak)

ctg25_38

Search NORINE for peptide: strict or relaxed

AMP-binding (467..857): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAlviGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 70% (weak)

ctg25_58

PKS_AT (534..831): pk

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 110.4
Malonyl-CoA: 108.6
Methoxymalonyl-CoA: 103.2
Ethylmalonyl-CoA: 96.1
trans-1,2-CPDA: 74.4
Isobutyryl-CoA: 70.7
Propionyl-CoA: 53.4
Acetyl-CoA: 52.0
Benzoyl-CoA: 51.0
fatty_acid: 50.7
2-Methylbutyryl-CoA: 41.5
CHC-CoA: 31.4
inactive: 27.3
3-Methylbutyryl-CoA: 26.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

ctg25_59

Search NORINE for peptide: strict or relaxed

AMP-binding (474..880): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)