3300025925_12 - 11 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300025.. (original name was: 3300025925.a:Ga0207650_10000041)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	bacteriocin	79,827	91,665

c00006_3300025.. (original name was: 3300025925.a:Ga0207650_10000007)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	lassopeptide	167,260	190,005

c00008_3300025.. (original name was: 3300025925.a:Ga0207650_10000269)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	bacteriocin	1	9,610

c00010_3300025.. (original name was: 3300025925.a:Ga0207650_10000005)

Region	Type	From	To
Region 10.1	NRPS,T1PKS	261	55,412
Region 10.2	lanthipeptide,NRPS,T1PKS	107,568	268,116
Region 10.3	lassopeptide	273,752	295,811

c00017_3300025.. (original name was: 3300025925.a:Ga0207650_10000080)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	NRPS-like	23,466	67,881

c00021_3300025.. (original name was: 3300025925.a:Ga0207650_10000009)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	NRPS-like	261,185	300,326
Region 21.2	T1PKS,NRPS	432,875	483,583

c00023_3300025.. (original name was: 3300025925.a:Ga0207650_10000022)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	NRPS-like	78,677	120,359
Region 23.2	NRPS-like	234,272	277,007

Region	Type	From	To	Similarity
Region 3.1	bacteriocin	79,827	91,665
Region 6.1	lassopeptide	167,260	190,005
Region 8.1	bacteriocin	1	9,610
Region 10.1	NRPS,T1PKS	261	55,412
Region 10.2	lanthipeptide,NRPS,T1PKS	107,568	268,116
Region 10.3	lassopeptide	273,752	295,811
Region 17.1	NRPS-like	23,466	67,881
Region 21.1	NRPS-like	261,185	300,326
Region 21.2	T1PKS,NRPS	432,875	483,583
Region 23.1	NRPS-like	78,677	120,359
Region 23.2	NRPS-like	234,272	277,007

No secondary metabolite regions were found in these records:: c00001_3300025.. (original name was: 3300025925.a:Ga0207650_10044685); c00002_3300025.. (original name was: 3300025925.a:Ga0207650_10000156); c00004_3300025.. (original name was: 3300025925.a:Ga0207650_10000087); c00005_3300025.. (original name was: 3300025925.a:Ga0207650_10043999); c00007_3300025.. (original name was: 3300025925.a:Ga0207650_10000927); c00009_3300025.. (original name was: 3300025925.a:Ga0207650_10000027); c00011_3300025.. (original name was: 3300025925.a:Ga0207650_10000028); c00012_3300025.. (original name was: 3300025925.a:Ga0207650_10000143); c00013_3300025.. (original name was: 3300025925.a:Ga0207650_10000006); c00014_3300025.. (original name was: 3300025925.a:Ga0207650_10050163); c00015_3300025.. (original name was: 3300025925.a:Ga0207650_10000061); c00016_3300025.. (original name was: 3300025925.a:Ga0207650_10000011); c00018_3300025.. (original name was: 3300025925.a:Ga0207650_10000332); c00019_3300025.. (original name was: 3300025925.a:Ga0207650_10000047); c00020_3300025.. (original name was: 3300025925.a:Ga0207650_10000017); c00022_3300025.. (original name was: 3300025925.a:Ga0207650_10000112); c00024_3300025.. (original name was: 3300025925.a:Ga0207650_10000313); c00025_3300025.. (original name was: 3300025925.a:Ga0207650_10000046)

Compact view

c00003_3300025.. - Region 1 - bacteriocin

Download region GenBank file

Location: 79,827 - 91,665 nt. (total: 11,839 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

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c00006_3300025.. - Region 1 - lassopeptide

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Location: 167,260 - 190,005 nt. (total: 22,746 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

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Lasso peptides

Lasso peptide predictions

Lassopeptides - No core peptides found.

Lasso peptides

Core peptide predictions

No core peptides found.

c00008_3300025.. - Region 1 - bacteriocin

Download region GenBank file

Location: 1 - 9,610 nt. (total: 9,610 nt) Show pHMM detection rules used

Region on contig edge.

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

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c00010_3300025.. - Region 1 - NRPS,T1PKS

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Location: 261 - 55,412 nt. (total: 55,152 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 260 - 55412:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ala - ala - gly - X) + (X - X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 260 - 19575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_4

PKS_AT (535..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 139.4
inactive: 92.8
Methoxymalonyl-CoA: 58.6
Methylmalonyl-CoA: 48.6
Ethylmalonyl-CoA: 28.4
Propionyl-CoA: 22.8
Isobutyryl-CoA: 22.8
CHC-CoA: 21.1
2-Methylbutyryl-CoA: 16.9
Benzoyl-CoA: 14.6
3-Methylbutyryl-CoA: 10.6
Acetyl-CoA: 8.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0

ctg10_6

Search NORINE for peptide: strict or relaxed

AMP-binding (471..871): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWHFgqIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

A-OX (1550..2316): X

ctg10_7

Search NORINE for peptide: strict or relaxed

CAL_domain (37..486): pk

minowa: NH2

Prediction, score:

NH2: 184.3
AHBA: 17.0
fatty_acid: 13.2
shikimic_acid: 12.0
Acetyl-CoA: -1.8

AMP-binding (1161..1557): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

AMP-binding (2219..2615): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

AMP-binding (3269..3664): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (4742..5142): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWHFgqIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

c00010_3300025.. - Region 2 - NRPS,T1PKS,lanthipeptide

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Location: 107,568 - 268,116 nt. (total: 160,549 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS domains

Lanthipeptides

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

NRPS/PKS products

NRPS/PKS monomers

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 3, location 107567 - 268116:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu - ala) + (X - D-thr) + (gln) + (gln) + (asn) + (ccmal - gln) + (gln - gln - D-X) + (asp) + (thr) + (gly - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 139240 - 268116:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu - ala) + (X - D-thr) + (gln) + (gln) + (asn) + (ccmal - gln) + (gln - gln - D-X) + (asp) + (thr) + (gly - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_116

Search NORINE for peptide: strict or relaxed

AMP-binding (458..853): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1515..1924): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVrHFaLlaK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 60% (weak)

AMP-binding (3000..3225): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: esWHFScgvK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg10_117

Search NORINE for peptide: strict or relaxed

AMP-binding (983..1381): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg10_118

Search NORINE for peptide: strict or relaxed

AMP-binding (441..839): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg10_119

Search NORINE for peptide: strict or relaxed

AMP-binding (935..1335): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQaGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

AMP-binding (2001..2402): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

AMP-binding (3064..3470): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeDVGAvtK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 60% (weak)

ctg10_120

Search NORINE for peptide: strict or relaxed

PKS_AT (537..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.5
inactive: 108.0
Methoxymalonyl-CoA: 75.1
Methylmalonyl-CoA: 63.7
Propionyl-CoA: 53.6
Ethylmalonyl-CoA: 41.7
2-Methylbutyryl-CoA: 32.4
CHC-CoA: 29.4
Isobutyryl-CoA: 25.5
Acetyl-CoA: 17.2
trans-1,2-CPDA: 14.9
Benzoyl-CoA: 14.9
fatty_acid: 10.1
3-Methylbutyryl-CoA: 6.7

AMP-binding (1975..2376): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

ctg10_121

Search NORINE for peptide: strict or relaxed

AMP-binding (460..862): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg10_123

Search NORINE for peptide: strict or relaxed

AMP-binding (460..862): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

ctg10_124

Search NORINE for peptide: strict or relaxed

AMP-binding (457..854): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

ctg10_126

Search NORINE for peptide: strict or relaxed

AMP-binding (463..876): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHfvyraK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 50% (weak)

AMP-binding (1538..1945): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg10_132

Search NORINE for peptide: strict or relaxed

CAL_domain (176..498): pk

minowa: NH2

Prediction, score:

NH2: 173.9
Acetyl-CoA: 34.1
fatty_acid: 19.6
shikimic_acid: 0.0
AHBA: 0.0

AMP-binding (1168..1565): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGhVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 90% (moderate)

AMP-binding (2221..2614): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

c00010_3300025.. - Region 3 - lassopeptide

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Location: 273,752 - 295,811 nt. (total: 22,060 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

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Lasso peptides

Lasso peptide predictions

Lassopeptide(s) for ctg10_158 - Putative Class II

ctg10_158 leader / core peptide / cleaved tail: MQNNYEAPELTLIGEANEVVM - GAAIGGNDFPKEFGLDFEFEQD

Legend:

Red: Putative macrolactam
Purple: Putative cleaved off residues

Lasso peptides

Core peptide predictions

ctg10_158 - Class II: Cleavage pHMM score: -9.50; RODEO score: 15; Monoisotopic mass: 2384.1 Da; Molecular weight: 2385.5 Da; Number of bridges: 0

c00017_3300025.. - Region 1 - NRPS-like

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Location: 23,466 - 67,881 nt. (total: 44,416 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 23465 - 67881:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg17_42

Search NORINE for peptide: strict or relaxed

AMP-binding (570..1006): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: eFtviGGpVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

c00021_3300025.. - Region 1 - NRPS-like

Download region GenBank file

Location: 261,185 - 300,326 nt. (total: 39,142 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 261184 - 300326:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg21_204

Search NORINE for peptide: strict or relaxed

AMP-binding (10..409): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: vfmvIGGslK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 40% (weak)

c00021_3300025.. - Region 2 - NRPS,T1PKS

Download region GenBank file

Location: 432,875 - 483,583 nt. (total: 50,709 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 432874 - 483583:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 432874 - 478769:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 457719 - 483583:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg21_347

PKS_AT (615..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Propionyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 95.2
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 57.2
inactive: 44.2
Propionyl-CoA: 26.9
Ethylmalonyl-CoA: 21.3
Isobutyryl-CoA: 20.0
CHC-CoA: 19.9
trans-1,2-CPDA: 14.6
fatty_acid: 14.4
Benzoyl-CoA: 13.3
Acetyl-CoA: 9.3
2-Methylbutyryl-CoA: 8.2
3-Methylbutyryl-CoA: 0.0

ctg21_362

Search NORINE for peptide: strict or relaxed

AMP-binding (496..895): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWlGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

c00023_3300025.. - Region 1 - NRPS-like

Download region GenBank file

Location: 78,677 - 120,359 nt. (total: 41,683 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 78676 - 120359:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg23_95

Search NORINE for peptide: strict or relaxed

AMP-binding (17..420): asn

NRPSPredictor2: asn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGhVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)

c00023_3300025.. - Region 2 - NRPS-like

Download region GenBank file

Location: 234,272 - 277,007 nt. (total: 42,736 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 234271 - 277007:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg23_232

Search NORINE for peptide: strict or relaxed

AMP-binding (35..439): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGhVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)