LOCUS       c00476_3300026..        3874 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  3300026144.a:Ga0209954_1002023.
ACCESSION   c00476_3300026..
VERSION     c00476_3300026..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 5.1.0
            Run date     :: 2019-11-23 13:43:48
            Original ID  :: 3300026144.a:Ga0209954_1002023
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3874
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3874
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[844:3874](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(Condensation and (AMP-binding or
                     A-OX)) or (Condensation and AMP-binding))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      845..3874
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(Condensation and (AMP-binding or
                     A-OX)) or (Condensation and AMP-binding))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..3874
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cds(Condensation and (AMP-binding or
                     A-OX)) or (Condensation and AMP-binding))"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..3874
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="(cds(Condensation and (AMP-binding or A-OX)) or
                     (Condensation and AMP-binding))"
                     /tool="antismash"
     CDS             845..3874
                     /NRPS_PKS="Domain: CAL_domain (50-508). E-value: 3e-124.
                     Score: 406.9. Matches aSDomain:
                     nrpspksdomains_ctg476_1_CAL_domain.1"
                     /NRPS_PKS="Domain: PP-binding (664-724). E-value: 6.4e-14.
                     Score: 44.0. Matches aSDomain:
                     nrpspksdomains_ctg476_1_PP-binding.1"
                     /NRPS_PKS="Domain: Condensation_LCL (754-1009). E-value:
                     2.2e-54. Score: 176.2. Matches aSDomain:
                     nrpspksdomains_ctg476_1_Condensation_LCL.1"
                     /NRPS_PKS="type: NRPS-like protein"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     401.9; E-value: 5.4e-122)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg476_1"
                     /sec_met_domain="Condensation (E-value: 4.4e-38, bitscore:
                     117.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-96, bitscore:
                     309.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.3e-15, bitscore:
                     44.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHEIASSSDAQETCGVSLADFLEAHARRQPERTLYTWLDGSGEET
                     DSLTRGDLDLRARAVAAHLTGEAGLVAGDRALLLFAPGLEFIAAFLGCLYAGVVAVPAY
                     PPSRRSMARLTGIAGDAEPGAVLTTASLLPRVASWLDEQPASGPLAGATPVAVDEVWAG
                     APESVAAWERPPREGSDLAFLQYTSGSTGTPKGVRVTHANLVHNEEMIRRAFDQSEESV
                     IVSWLPLYHDMGLIGGVLQPLYLGARCVLTSPAAFLRRPRLWLEAIHRYRGTTSGGPDF
                     GYALVEAKVGPEEREGLDLSSWSVAFNGSEPVRAATLERFARAFAPHGFHRRAFYPCYG
                     MAEATLLVSGGDAAAEPVVGRYDTTALEGHEAVETAADDAGNRDLVGCGRTWRGQRLEI
                     VDPESRRALPAGRVGEIWVAGPSVADGYWRQPEATEHAFDGRLAGVARDEDEADGPFLR
                     TGDLGFLGAGDELFVTGRLKDLIILRGRNVYPHDVERTAEAAHDAFRAGCSAAFPVELD
                     GEERMVLAVEVDRHFAAEDPAAAREATDAARRAVFEEHEAALHEVVLLRVGTLPKTSSG
                     KVRRHACRAAYLGDGLAGAMAPKAGAVDTPEADAATGAGTVAAPEVATPEVAPVTPAPA
                     TPDLPSRADLAGLDAGGRQRALRGPLTAWAAAALRRRADDLDPAAPLIAQGLDSLSAAE
                     LLARVEAELGVDLDFGDLLEGASVEELAALAAGALVDGAGSASAAIQTEPFETRGAPAP
                     GPLPLSRGQAALWFLHRLDPSSAAYHITVGARVAGDLDVAALERALLALARRHPQLRAT
                     FHDLDGEPVQRVAPPAAAAVELLRPPRPLEAAGRDAAPWGDEAARARLLAEARRPFDLT
                     RGPLLRAVVLPRGGDGEGGAGADLALVVHHLVADFWSLVVLFEELETLYTAEVSEAPAA
                     ADAASLGAPAASFADFVDWERRRLAGPEGEALEAWWREALSDGAVADLDLPTDRPRPAV
                     LSDRGGAVAAPLGEAATRAVE"
     aSDomain        995..2368
                     /aSDomain="CAL_domain"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg476_1_CAL_domain.1"
                     /evalue="3.00E-124"
                     /label="ctg476_1_CAL_domain.1"
                     /locus_tag="ctg476_1"
                     /protein_end="508"
                     /protein_start="50"
                     /score="406.9"
                     /specificity="Minowa: NH2"
                     /tool="antismash"
                     /translation="GDLDLRARAVAAHLTGEAGLVAGDRALLLFAPGLEFIAAFLGCLY
                     AGVVAVPAYPPSRRSMARLTGIAGDAEPGAVLTTASLLPRVASWLDEQPASGPLAGATP
                     VAVDEVWAGAPESVAAWERPPREGSDLAFLQYTSGSTGTPKGVRVTHANLVHNEEMIRR
                     AFDQSEESVIVSWLPLYHDMGLIGGVLQPLYLGARCVLTSPAAFLRRPRLWLEAIHRYR
                     GTTSGGPDFGYALVEAKVGPEEREGLDLSSWSVAFNGSEPVRAATLERFARAFAPHGFH
                     RRAFYPCYGMAEATLLVSGGDAAAEPVVGRYDTTALEGHEAVETAADDAGNRDLVGCGR
                     TWRGQRLEIVDPESRRALPAGRVGEIWVAGPSVADGYWRQPEATEHAFDGRLAGVARDE
                     DEADGPFLRTGDLGFLGAGDELFVTGRLKDLIILRGRNVYPHDVERTAEAAHDAFRAGC
                     S"
     aSModule        995..3016
                     /domains="nrpspksdomains_ctg476_1_CAL_domain.1"
                     /domains="nrpspksdomains_ctg476_1_PP-binding.1"
                     /incomplete
                     /locus_tags="ctg476_1"
                     /starter_module
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       1388..1447
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0001"
                     /evalue="2.00E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="ctg476_1"
                     /protein_end="201"
                     /protein_start="181"
                     /score="26.4"
                     /tool="antismash"
                     /translation="AFLQYTSGSTGTPKGVRVTHA"
     CDS_motif       2069..2158
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0002"
                     /evalue="6.90E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="ctg476_1"
                     /protein_end="438"
                     /protein_start="408"
                     /score="40.8"
                     /tool="antismash"
                     /translation="PAGRVGEIWVAGPSVADGYWRQPEATEHAFD"
     CDS_motif       2264..2326
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0003"
                     /evalue="9.70E-05"
                     /label="NRPS-A_a8"
                     /locus_tag="ctg476_1"
                     /protein_end="494"
                     /protein_start="473"
                     /score="15.1"
                     /tool="antismash"
                     /translation="TGRLKDLIILRGRNVYPHDVER"
     aSDomain        2837..3016
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg476_1_PP-binding.1"
                     /evalue="6.40E-14"
                     /label="ctg476_1_PP-binding.1"
                     /locus_tag="ctg476_1"
                     /protein_end="724"
                     /protein_start="664"
                     /score="44.0"
                     /tool="antismash"
                     /translation="AAAALRRRADDLDPAAPLIAQGLDSLSAAELLARVEAELGVDLDF
                     GDLLEGASVEELAALA"
     aSDomain        3107..3871
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg476_1_Condensation_LCL.1"
                     /domain_subtype="Condensation_LCL"
                     /evalue="2.20E-54"
                     /label="ctg476_1_Condensation_LCL.1"
                     /locus_tag="ctg476_1"
                     /protein_end="1009"
                     /protein_start="754"
                     /score="176.2"
                     /tool="antismash"
                     /translation="PLPLSRGQAALWFLHRLDPSSAAYHITVGARVAGDLDVAALERAL
                     LALARRHPQLRATFHDLDGEPVQRVAPPAAAAVELLRPPRPLEAAGRDAAPWGDEAARA
                     RLLAEARRPFDLTRGPLLRAVVLPRGGDGEGGAGADLALVVHHLVADFWSLVVLFEELE
                     TLYTAEVSEAPAAADAASLGAPAASFADFVDWERRRLAGPEGEALEAWWREALSDGAVA
                     DLDLPTDRPRPAVLSDRGGAVAAPLGEAATRAVE"
     aSModule        3107..3871
                     /domains="nrpspksdomains_ctg476_1_Condensation_LCL.1"
                     /incomplete
                     /locus_tags="ctg476_1"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3170..3286
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0004"
                     /evalue="1.00E-13"
                     /label="C2_LCL_024-062"
                     /locus_tag="ctg476_1"
                     /protein_end="814"
                     /protein_start="775"
                     /score="43.7"
                     /tool="antismash"
                     /translation="AAYHITVGARVAGDLDVAALERALLALARRHPQLRATFHD"
     CDS_motif       3464..3496
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0005"
                     /evalue="2.70E-01"
                     /label="Cy2"
                     /locus_tag="ctg476_1"
                     /protein_end="884"
                     /protein_start="873"
                     /score="4.2"
                     /tool="antismash"
                     /translation="PLLRAVVLPRGG"
     CDS_motif       3539..3604
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg476_1_0006"
                     /evalue="5.50E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="ctg476_1"
                     /protein_end="920"
                     /protein_start="898"
                     /score="25.4"
                     /tool="antismash"
                     /translation="VHHLVADFWSLVVLFEELETLYT"
     misc_feature    3599..3601
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gcggtgtctc ttctatgggt cgtggggcgg tgcggccagg tcggggccgg cgccgcctct
       61 cggtttcggt ggtctcgtct caccgacgcg ggtggcgggg cgcttcgtgg gccgcccgcc
      121 gggcgtcggt ctaccactat ctacagcatt gcttgtgcca ggtttcaatg cagtctgaaa
      181 tatcttgtcg cagaagggga ttgtagagtt ctgcggtggc tggcgacagc ggcaagctgc
      241 ccacgggcgt ccggtggcgc ggagtatggt cccaaaatcg gacggttgcc cgcttcactc
      301 agttaggtgc gccgcggcgg gcggaccggg acagccccgc acgacggccc ggagccgccg
      361 ggcgggtgcc gatcctggaa agatttgaca aagttgtcac ggtcccgtgt ttttttggca
      421 cttacgagtt gagacagcag cattagagcc aaatggcgat gatgcgaaga agtctatgtc
      481 tggcgactcg gtctagcgat gcgcagtcgc cggggcgccg gattcactag cccgggcgac
      541 tctagaagaa gtaccccagc cggtccagac actccacgcg acgtgctccc acggaccgat
      601 acgttgggag agcttgaacc agtctccacg aaatcgtctt tcgacgtggg ggcgtccctt
      661 gcccggggca gccttggttt cggctcatct ttccccgcta gcacgggtga gctgcacccc
      721 cacccactcg cttgcgagat ttaataagag ctaaatactg caacagttcg cagattattt
      781 cgttaccaag ttagtgaaga ataccgcagt gatgaacaac gtcgataagc caggtgccgt
      841 acctatgcac gagatcgcca gcagcagtga cgctcaggaa acttgcgggg tgagcctcgc
      901 cgatttcctg gaggcccacg cccggcgtca gccggagcgc accctctaca cctggctgga
      961 cggctcgggg gaggagaccg actcgctgac ccggggcgac ctcgatctgc gcgcccgggc
     1021 ggtggcggcc cacctgaccg gcgaggccgg gctggtggcc ggcgaccggg cccttctgct
     1081 cttcgccccc ggcctggagt tcatcgccgc cttcctcggc tgcctctacg ccggcgtcgt
     1141 ggcggtgccc gcctacccgc ccagccggcg ctccatggca cgcctcaccg gcatcgccgg
     1201 cgacgccgag ccgggggcgg tgctgaccac cgcctccctg ctgccccggg tcgcctcctg
     1261 gctggacgag cagcccgctt ccgggccgtt ggccggcgcc acgccggtgg cggtggacga
     1321 ggtgtgggcc ggggcgccgg agtcggtggc ggcgtgggag cgcccgcccc gggagggctc
     1381 cgacctggcc ttcctccagt acacctccgg ctccaccggc acccccaagg gcgtgcgggt
     1441 gacccacgcc aacctggttc acaacgagga gatgatccgc cgcgccttcg accagtcgga
     1501 ggagtcggtc atcgtctcct ggctgcccct ctaccacgac atggggctca tcggcggcgt
     1561 cctgcagccg ctctacctgg gggcgcgctg cgtgctgacc tcgccggcgg cgttcctgcg
     1621 ccgcccccgc ctctggctgg aggccatcca ccgctaccgg ggcaccacca gcggcggccc
     1681 cgacttcggc tacgccctgg tggaggcgaa ggtcggcccg gaggagcggg aggggctgga
     1741 cctgtcctcc tggagcgtcg ccttcaacgg ctccgagccg gtgcgcgccg cgaccctgga
     1801 gcgcttcgcc cgcgccttcg ccccccacgg cttccaccgc cgggcgtttt acccctgcta
     1861 cggcatggcc gaggccaccc tgctggtctc cggcggcgac gccgccgccg agccggtggt
     1921 ggggcgctac gacaccaccg ccctggaggg gcacgaggct gtggagaccg ccgccgacga
     1981 cgccggaaac cgcgacctgg tgggctgcgg ccgcacctgg cggggccagc gactggagat
     2041 cgtcgatccg gagagccgcc gggccctgcc ggcgggccgg gtgggggaga tctgggtcgc
     2101 cggccccagc gtcgccgacg gctactggcg ccaacccgag gcgacggagc acgccttcga
     2161 cggccgcctg gccggcgtgg cgcgggacga ggacgaggcc gacgggccgt tcctgcgcac
     2221 cggcgacctc ggtttcctcg gcgccggcga cgagctgttc gtcaccgggc gcctgaagga
     2281 cctgatcatc ctgcgcggcc gcaacgtcta cccccacgac gtggagcgca ccgccgaggc
     2341 cgcccacgac gccttccgcg ccggctgtag cgccgccttc ccggtggaac tggacgggga
     2401 ggagcggatg gtcctggcgg tggaggtgga tcgccacttc gccgccgagg accccgccgc
     2461 tgcccgggag gccaccgacg cggcgcgccg ggccgtcttc gaagagcacg aggcggcgct
     2521 ccacgaggtg gtcctgctgc gggtcggcac cctgcccaag acctccagcg gcaaggtccg
     2581 ccgccacgcc tgccgcgccg cctacctggg cgacggcctg gccggcgcca tggcgccgaa
     2641 ggccggcgcg gtggacacgc ctgaggccga tgccgccacc ggagctggca cggtagccgc
     2701 ccccgaagtg gcgacccccg aagtggcccc cgtcacgccg gcccccgcca cccccgacct
     2761 cccctcccgg gccgacctgg ccggcctcga cgccggtggc cgccagcgcg ccctgcgcgg
     2821 cccgctgacc gcctgggccg ccgccgccct gcgccgccgc gccgatgatc tcgaccccgc
     2881 cgcccccttg atcgcccagg gcctggactc cctgtccgcc gccgagctgc tggcccgggt
     2941 cgaggcggag ctgggggtcg atctcgactt cggcgacctg ctggaggggg cgtcggtgga
     3001 ggagctggcg gcgcttgccg ccggggcgct ggtcgatggc gcaggctccg ccagcgccgc
     3061 catccagacc gagcctttcg aaacccgggg cgccccggcc cccggcccgt tgccgctgtc
     3121 ccggggccag gcggcgctct ggttcctgca ccggctggac ccgtcctcgg ccgcctacca
     3181 catcaccgtc ggcgcccggg tggcgggcga cctggacgtg gcggcgctgg aacgcgccct
     3241 cctcgccctg gcccgccgcc acccccagct ccgcgccacc ttccacgacc tggacggcga
     3301 gccggtgcag cgcgtcgccc cgccggcggc cgcggcggtg gagctgttgc ggccgccgcg
     3361 gcctctggag gctgcgggcc gggacgcggc gccctggggc gacgaagccg cccgcgcccg
     3421 cctcctggcc gaggcccgcc gccccttcga cctgacccgg ggcccgctgc tgcgcgccgt
     3481 cgtcctgccc cggggcgggg acggtgaagg tggggccggc gccgacctcg ccctggtggt
     3541 ccaccacctg gtggccgact tctggtcgct ggtggtcctg ttcgaggagc tggagacctt
     3601 atatacagcg gaggtcagcg aggccccggc cgccgccgac gccgcctccc tgggcgcccc
     3661 ggctgcgagc ttcgccgact tcgtcgattg ggagcgccgc cgcctggccg ggccggaggg
     3721 ggaggcgctg gaggcgtggt ggcgcgaggc gctgtccgac ggcgccgtcg ccgacctcga
     3781 cctgcccacc gaccgccccc gccccgccgt cctcagcgac cgcggcggcg ccgtcgccgc
     3841 gccgctgggc gaggcggcga cccgggcggt ggag
//
