3300026272_2 - 7 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00004_3300026.. (original name was: 3300026272.a:Ga0209913_1000065)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	arylpolyene	61,389	105,020

c00034_3300026.. (original name was: 3300026272.a:Ga0209913_1000083)

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	NRPS	1	51,546

c00045_3300026.. (original name was: 3300026272.a:Ga0209913_1000086)

Region	Type	From	To	Most similar known cluster		Similarity
Region 45.1	terpene	11,811	37,460

c00063_3300026.. (original name was: 3300026272.a:Ga0209913_1001680)

Region	Type	From	To	Most similar known cluster		Similarity
Region 63.1	phosphonate	1	11,340

c00067_3300026.. (original name was: 3300026272.a:Ga0209913_1000005)

Region	Type	From	To	Most similar known cluster		Similarity
Region 67.1	thiopeptide	48,455	91,712
Region 67.2	NRPS,T1PKS	308,162	364,613

c00071_3300026.. (original name was: 3300026272.a:Ga0209913_1000346)

Region	Type	From	To	Most similar known cluster		Similarity
Region 71.1	bacteriocin	1	9,002

Region	Type	From	To	Similarity
Region 4.1	arylpolyene	61,389	105,020
Region 34.1	NRPS	1	51,546
Region 45.1	terpene	11,811	37,460
Region 63.1	phosphonate	1	11,340
Region 67.1	thiopeptide	48,455	91,712
Region 67.2	NRPS,T1PKS	308,162	364,613
Region 71.1	bacteriocin	1	9,002

No secondary metabolite regions were found in these records:: c00001_3300026.. (original name was: 3300026272.a:Ga0209913_1009224); c00002_3300026.. (original name was: 3300026272.a:Ga0209913_1006030); c00003_3300026.. (original name was: 3300026272.a:Ga0209913_1000050); c00005_3300026.. (original name was: 3300026272.a:Ga0209913_1004265); c00006_3300026.. (original name was: 3300026272.a:Ga0209913_1000033); c00007_3300026.. (original name was: 3300026272.a:Ga0209913_1006246); c00008_3300026.. (original name was: 3300026272.a:Ga0209913_1004714); c00009_3300026.. (original name was: 3300026272.a:Ga0209913_1000109); c00010_3300026.. (original name was: 3300026272.a:Ga0209913_1000012); c00011_3300026.. (original name was: 3300026272.a:Ga0209913_1000071); c00012_3300026.. (original name was: 3300026272.a:Ga0209913_1000396); c00013_3300026.. (original name was: 3300026272.a:Ga0209913_1000069); c00014_3300026.. (original name was: 3300026272.a:Ga0209913_1001730); c00015_3300026.. (original name was: 3300026272.a:Ga0209913_1003910); c00016_3300026.. (original name was: 3300026272.a:Ga0209913_1000766); c00017_3300026.. (original name was: 3300026272.a:Ga0209913_1000437); c00018_3300026.. (original name was: 3300026272.a:Ga0209913_1000231); c00019_3300026.. (original name was: 3300026272.a:Ga0209913_1000090); c00020_3300026.. (original name was: 3300026272.a:Ga0209913_1000449); c00021_3300026.. (original name was: 3300026272.a:Ga0209913_1000123); c00022_3300026.. (original name was: 3300026272.a:Ga0209913_1000026); c00023_3300026.. (original name was: 3300026272.a:Ga0209913_1009322); c00024_3300026.. (original name was: 3300026272.a:Ga0209913_1009128); c00025_3300026.. (original name was: 3300026272.a:Ga0209913_1002665); c00026_3300026.. (original name was: 3300026272.a:Ga0209913_1001080); c00027_3300026.. (original name was: 3300026272.a:Ga0209913_1000970); c00028_3300026.. (original name was: 3300026272.a:Ga0209913_1000077); c00029_3300026.. (original name was: 3300026272.a:Ga0209913_1000119); c00030_3300026.. (original name was: 3300026272.a:Ga0209913_1000136); c00031_3300026.. (original name was: 3300026272.a:Ga0209913_1001106); c00032_3300026.. (original name was: 3300026272.a:Ga0209913_1001903); c00033_3300026.. (original name was: 3300026272.a:Ga0209913_1000157); c00035_3300026.. (original name was: 3300026272.a:Ga0209913_1000036); c00036_3300026.. (original name was: 3300026272.a:Ga0209913_1001597); c00037_3300026.. (original name was: 3300026272.a:Ga0209913_1001112); c00038_3300026.. (original name was: 3300026272.a:Ga0209913_1003179); c00039_3300026.. (original name was: 3300026272.a:Ga0209913_1000172); c00040_3300026.. (original name was: 3300026272.a:Ga0209913_1000125); c00041_3300026.. (original name was: 3300026272.a:Ga0209913_1000138); c00042_3300026.. (original name was: 3300026272.a:Ga0209913_1001165); c00043_3300026.. (original name was: 3300026272.a:Ga0209913_1002315); c00044_3300026.. (original name was: 3300026272.a:Ga0209913_1002671); c00046_3300026.. (original name was: 3300026272.a:Ga0209913_1000141); c00047_3300026.. (original name was: 3300026272.a:Ga0209913_1000088); c00048_3300026.. (original name was: 3300026272.a:Ga0209913_1000282); c00049_3300026.. (original name was: 3300026272.a:Ga0209913_1001308); c00050_3300026.. (original name was: 3300026272.a:Ga0209913_1000435); c00051_3300026.. (original name was: 3300026272.a:Ga0209913_1000047); c00052_3300026.. (original name was: 3300026272.a:Ga0209913_1000100); c00053_3300026.. (original name was: 3300026272.a:Ga0209913_1000089); c00054_3300026.. (original name was: 3300026272.a:Ga0209913_1000380); c00055_3300026.. (original name was: 3300026272.a:Ga0209913_1000066); c00056_3300026.. (original name was: 3300026272.a:Ga0209913_1000348); c00057_3300026.. (original name was: 3300026272.a:Ga0209913_1000196); c00058_3300026.. (original name was: 3300026272.a:Ga0209913_1000002); c00059_3300026.. (original name was: 3300026272.a:Ga0209913_1001130); c00060_3300026.. (original name was: 3300026272.a:Ga0209913_1000506); c00061_3300026.. (original name was: 3300026272.a:Ga0209913_1000021); c00062_3300026.. (original name was: 3300026272.a:Ga0209913_1000068); c00064_3300026.. (original name was: 3300026272.a:Ga0209913_1000481); c00065_3300026.. (original name was: 3300026272.a:Ga0209913_1000153); c00066_3300026.. (original name was: 3300026272.a:Ga0209913_1000056); c00068_3300026.. (original name was: 3300026272.a:Ga0209913_1000170); c00069_3300026.. (original name was: 3300026272.a:Ga0209913_1000017); c00070_3300026.. (original name was: 3300026272.a:Ga0209913_1000637); c00072_3300026.. (original name was: 3300026272.a:Ga0209913_1000132)

Compact view

c00004_3300026.. - Region 1 - arylpolyene

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Location: 61,389 - 105,020 nt. (total: 43,632 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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c00034_3300026.. - Region 1 - NRPS

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Location: 1 - 51,546 nt. (total: 51,546 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 51546:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-asp - ser) + (asp) + (D-thr - ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg34_3

Search NORINE for peptide: strict or relaxed

AMP-binding (495..898): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2009..2415): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (3089..3496): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGevVGGvtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)

ctg34_4

Search NORINE for peptide: strict or relaxed

AMP-binding (433..827): asp

NRPSPredictor2: asp

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg34_5

Search NORINE for peptide: strict or relaxed

CAL_domain (43..484): pk

minowa: NH2

Prediction, score:

NH2: 160.6
fatty_acid: 31.5
Acetyl-CoA: 7.0
shikimic_acid: 0.0
AHBA: 0.0

AMP-binding (1155..1558): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

AMP-binding (2698..3105): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

c00045_3300026.. - Region 1 - terpene

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Location: 11,811 - 37,460 nt. (total: 25,650 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

additional biosynthetic genes

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c00063_3300026.. - Region 1 - phosphonate

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Location: 1 - 11,340 nt. (total: 11,340 nt) Show pHMM detection rules used

Region on contig edge.

phosphonate: phosphonates

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core biosynthetic genes

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c00067_3300026.. - Region 1 - thiopeptide

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Location: 48,455 - 91,712 nt. (total: 43,258 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03882) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

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core biosynthetic genes

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Thiopeptides

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.

c00067_3300026.. - Region 2 - NRPS,T1PKS

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Location: 308,162 - 364,613 nt. (total: 56,452 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 308161 - 364613:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ala-b - trp - ohmal) + (D-X - D-phe)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg67_304

CAL_domain (173..580): pk

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 12.6
fatty_acid: 12.0
NH2: 8.3
shikimic_acid: 0.0
AHBA: 0.0

ctg67_305

Search NORINE for peptide: strict or relaxed

AMP-binding (505..905): ala-b

NRPSPredictor2: ala-b

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDWtISLADK
Nearest Stachelhaus code:: ala-b
Stachelhaus code match:: 90% (moderate)

AMP-binding (1554..1948): trp

NRPSPredictor2: trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSqIGAVtK
Nearest Stachelhaus code:: trp
Stachelhaus code match:: 80% (moderate)

PKS_AT (2676..2959): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 168.2
inactive: 118.3
Methoxymalonyl-CoA: 74.5
Methylmalonyl-CoA: 64.0
Propionyl-CoA: 40.6
Ethylmalonyl-CoA: 39.1
2-Methylbutyryl-CoA: 23.6
CHC-CoA: 19.7
trans-1,2-CPDA: 18.5
Acetyl-CoA: 14.4
Isobutyryl-CoA: 13.7
fatty_acid: 9.7
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg67_306

Search NORINE for peptide: strict or relaxed

AMP-binding (534..936): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVenVGgItK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 60% (weak)

AMP-binding (2035..2428): phe

NRPSPredictor2: phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAgVaK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 80% (moderate)

c00071_3300026.. - Region 1 - bacteriocin

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Location: 1 - 9,002 nt. (total: 9,002 nt) Show pHMM detection rules used

Region on contig edge.

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it