3300026286_6 - 15 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00004_3300026.. (original name was: 3300026286.a:Ga0209460_1000018)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	377,320	411,814

c00005_3300026.. (original name was: 3300026286.a:Ga0209460_1000006)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	terpene	408,959	429,933

c00006_3300026.. (original name was: 3300026286.a:Ga0209460_1000007)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	thiopeptide,LAP	13,470	44,103
Region 6.2	bacteriocin	113,185	123,400

c00007_3300026.. (original name was: 3300026286.a:Ga0209460_1000013)

Region	Type	From	To	Most similar known cluster		Similarity
Region 7.1	NRPS,T1PKS	62,568	152,934
Region 7.2	NRPS,T1PKS,lanthipeptide	181,925	237,527

c00008_3300026.. (original name was: 3300026286.a:Ga0209460_1000024)

Region	Type	From	To	Most similar known cluster		Similarity
Region 8.1	T1PKS	50,972	96,698

c00009_3300026.. (original name was: 3300026286.a:Ga0209460_1000163)

Region	Type	From	To	Most similar known cluster		Similarity
Region 9.1	NRPS	4,147	60,082

c00010_3300026.. (original name was: 3300026286.a:Ga0209460_1000067)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	T1PKS,NRPS-like	1	55,300

c00013_3300026.. (original name was: 3300026286.a:Ga0209460_1000032)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	T1PKS,NRPS	200,846	271,347

c00014_3300026.. (original name was: 3300026286.a:Ga0209460_1000038)

Region	Type	From	To	Most similar known cluster		Similarity
Region 14.1	NRPS-like	224,156	247,083

c00015_3300026.. (original name was: 3300026286.a:Ga0209460_1000015)

Region	Type	From	To	Most similar known cluster		Similarity
Region 15.1	NRPS	344,929	393,572
Region 15.2	terpene	406,145	428,224

c00016_3300026.. (original name was: 3300026286.a:Ga0209460_1000035)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	terpene	167,016	188,026

c00017_3300026.. (original name was: 3300026286.a:Ga0209460_1000012)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	T3PKS	119,476	160,540

Region	Type	From	To	Similarity
Region 4.1	NRPS	377,320	411,814
Region 5.1	terpene	408,959	429,933
Region 6.1	thiopeptide,LAP	13,470	44,103
Region 6.2	bacteriocin	113,185	123,400
Region 7.1	NRPS,T1PKS	62,568	152,934
Region 7.2	NRPS,T1PKS,lanthipeptide	181,925	237,527
Region 8.1	T1PKS	50,972	96,698
Region 9.1	NRPS	4,147	60,082
Region 10.1	T1PKS,NRPS-like	1	55,300
Region 13.1	T1PKS,NRPS	200,846	271,347
Region 14.1	NRPS-like	224,156	247,083
Region 15.1	NRPS	344,929	393,572
Region 15.2	terpene	406,145	428,224
Region 16.1	terpene	167,016	188,026
Region 17.1	T3PKS	119,476	160,540

No secondary metabolite regions were found in these records:: c00001_3300026.. (original name was: 3300026286.a:Ga0209460_1000019); c00002_3300026.. (original name was: 3300026286.a:Ga0209460_1000017); c00003_3300026.. (original name was: 3300026286.a:Ga0209460_1005376); c00011_3300026.. (original name was: 3300026286.a:Ga0209460_1000044); c00012_3300026.. (original name was: 3300026286.a:Ga0209460_1000021)

Compact view

c00004_3300026.. - Region 1 - NRPS

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Location: 377,320 - 411,814 nt. (total: 34,495 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 377319 - 411814:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_289

Search NORINE for peptide: strict or relaxed

AMP-binding (37..441): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PMPglGVVNK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 80% (moderate)

ctg4_291

Search NORINE for peptide: strict or relaxed

AMP-binding (450..838): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAeDIGtVvK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 70% (weak)

c00005_3300026.. - Region 1 - terpene

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Location: 408,959 - 429,933 nt. (total: 20,975 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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c00006_3300026.. - Region 1 - LAP,thiopeptide

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Location: 13,470 - 44,103 nt. (total: 30,634 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03882) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)
LAP: ((goadsporin_like or PF00881 or TIGR03605) and (YcaO or TIGR03882))

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Thiopeptides

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.

c00006_3300026.. - Region 2 - bacteriocin

Download region GenBank file

Location: 113,185 - 123,400 nt. (total: 10,216 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

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core biosynthetic genes

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c00007_3300026.. - Region 1 - NRPS,T1PKS

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Location: 62,568 - 152,934 nt. (total: 90,367 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 62567 - 152934:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr - gly) + (asn) + (mal - X) + (D-asn) + (D-thr - ser) + (ohmal) + (D-orn - gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg7_59

Search NORINE for peptide: strict or relaxed

CAL_domain (63..485): pk

minowa: NH2

Prediction, score:

NH2: 197.5
Acetyl-CoA: 46.7
fatty_acid: 18.6
AHBA: 13.6
shikimic_acid: 2.0

AMP-binding (1135..1534): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2231..2619): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

ctg7_61

Search NORINE for peptide: strict or relaxed

AMP-binding (436..832): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (1491..1883): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLvAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

ctg7_67

CAL_domain (53..492): pk

minowa: NH2

Prediction, score:

NH2: 137.3
Acetyl-CoA: 33.7
fatty_acid: 26.1
shikimic_acid: 0.0
AHBA: 0.0

ctg7_68

Search NORINE for peptide: strict or relaxed

PKS_AT (623..913): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 140.6
inactive: 96.3
Methoxymalonyl-CoA: 46.8
Methylmalonyl-CoA: 40.4
Isobutyryl-CoA: 37.7
Propionyl-CoA: 26.9
2-Methylbutyryl-CoA: 25.0
Acetyl-CoA: 17.7
Benzoyl-CoA: 17.3
CHC-CoA: 15.5
Ethylmalonyl-CoA: 14.5
fatty_acid: 12.8
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 10.3

AMP-binding (2085..2497): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg7_69

Search NORINE for peptide: strict or relaxed

AMP-binding (475..886): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

ctg7_70

Search NORINE for peptide: strict or relaxed

AMP-binding (464..868): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1992..2385): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg7_71

PKS_AT (521..800): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 154.0
inactive: 95.9
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 46.3
Isobutyryl-CoA: 29.5
Ethylmalonyl-CoA: 23.7
Propionyl-CoA: 23.4
fatty_acid: 23.1
2-Methylbutyryl-CoA: 19.9
Benzoyl-CoA: 19.4
CHC-CoA: 16.5
Acetyl-CoA: 15.2
trans-1,2-CPDA: 10.3
3-Methylbutyryl-CoA: 0.0

ctg7_72

Search NORINE for peptide: strict or relaxed

AMP-binding (535..938): orn

NRPSPredictor2: orn

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: orn, horn
Single AA prediction:: orn

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code:: orn
Stachelhaus code match:: 100% (strong)

AMP-binding (2032..2433): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGmVWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 90% (moderate)

c00007_3300026.. - Region 2 - NRPS,T1PKS,lanthipeptide

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Location: 181,925 - 237,527 nt. (total: 55,603 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

Legend:

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NRPS/PKS domains

Lanthipeptides

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Lanthipeptides

NRPS/PKS products

NRPS/PKS monomers

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 2, location 181924 - 237527:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - asp)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 181924 - 234311:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg7_119

Search NORINE for peptide: strict or relaxed

CAL_domain (43..487): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 79.1
NH2: 38.1
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1230..1525): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 70.8%; 2-Methylbutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 85.0
Methylmalonyl-CoA: 60.1
Methoxymalonyl-CoA: 51.6
inactive: 38.7
Propionyl-CoA: 36.9
Ethylmalonyl-CoA: 19.0
CHC-CoA: 17.8
fatty_acid: 16.6
Isobutyryl-CoA: 13.8
Benzoyl-CoA: 13.2
trans-1,2-CPDA: 8.5
2-Methylbutyryl-CoA: 5.2
3-Methylbutyryl-CoA: 5.0
Acetyl-CoA: 0.0

AMP-binding (2674..3085): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DASrgGhInK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 60% (weak)

c00008_3300026.. - Region 1 - T1PKS

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Location: 50,972 - 96,698 nt. (total: 45,727 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

c00009_3300026.. - Region 1 - NRPS

Download region GenBank file

Location: 4,147 - 60,082 nt. (total: 55,936 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 4146 - 60082:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (thr) + (ala) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg9_27

Search NORINE for peptide: strict or relaxed

AMP-binding (1071..1467): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg9_28

Search NORINE for peptide: strict or relaxed

AMP-binding (528..929): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg9_29

Search NORINE for peptide: strict or relaxed

AMP-binding (12..399): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

ctg9_30

Search NORINE for peptide: strict or relaxed

AMP-binding (511..920): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

c00010_3300026.. - Region 1 - NRPS-like,T1PKS

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Location: 1 - 55,300 nt. (total: 55,300 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 55300:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Me-redmal) + (Me-redmal) + (ohmal - ohmal) + (ccmal) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 53082:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Me-redmal) + (Me-redmal) + (ohmal - ohmal) + (ccmal) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 13176 - 55300:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Me-redmal) + (Me-redmal) + (ohmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg10_1

PKS_AT (540..844): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 130.2
inactive: 79.7
Methoxymalonyl-CoA: 49.9
Methylmalonyl-CoA: 30.2
Propionyl-CoA: 25.5
Isobutyryl-CoA: 11.8
2-Methylbutyryl-CoA: 3.9
3-Methylbutyryl-CoA: 3.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

ctg10_2

PKS_AT (538..850): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 131.8
inactive: 101.8
Methoxymalonyl-CoA: 59.4
Methylmalonyl-CoA: 45.6
Propionyl-CoA: 26.4
Isobutyryl-CoA: 16.2
Ethylmalonyl-CoA: 15.3
Benzoyl-CoA: 12.4
fatty_acid: 10.2
trans-1,2-CPDA: 1.8
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg10_3

PKS_AT (553..861): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 127.7
inactive: 71.9
Methoxymalonyl-CoA: 49.5
Methylmalonyl-CoA: 34.4
Propionyl-CoA: 16.0
Acetyl-CoA: 7.6
3-Methylbutyryl-CoA: 7.6
Ethylmalonyl-CoA: 6.5
Isobutyryl-CoA: 4.3
2-Methylbutyryl-CoA: 3.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0

PKS_AT (2091..2399): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 137.5
inactive: 87.3
Methoxymalonyl-CoA: 55.6
Methylmalonyl-CoA: 34.1
Propionyl-CoA: 16.7
2-Methylbutyryl-CoA: 12.3
Isobutyryl-CoA: 11.1
Acetyl-CoA: 7.8
fatty_acid: 7.0
3-Methylbutyryl-CoA: 6.5
Ethylmalonyl-CoA: 3.9
CHC-CoA: 2.7
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0

ctg10_4

PKS_AT (600..895): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 179.0
Ethylmalonyl-CoA: 176.1
Methoxymalonyl-CoA: 141.9
Malonyl-CoA: 110.9
2-Methylbutyryl-CoA: 109.1
Isobutyryl-CoA: 106.1
Propionyl-CoA: 94.3
Benzoyl-CoA: 93.4
trans-1,2-CPDA: 85.5
Acetyl-CoA: 73.7
CHC-CoA: 54.2
fatty_acid: 46.1
3-Methylbutyryl-CoA: 35.0
inactive: 33.4

ctg10_5

PKS_AT (570..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.2
Ethylmalonyl-CoA: 153.3
Methoxymalonyl-CoA: 140.0
Malonyl-CoA: 112.3
Isobutyryl-CoA: 95.2
trans-1,2-CPDA: 87.1
Acetyl-CoA: 85.9
2-Methylbutyryl-CoA: 85.8
Propionyl-CoA: 84.0
Benzoyl-CoA: 82.5
CHC-CoA: 50.1
inactive: 38.4
fatty_acid: 34.6
3-Methylbutyryl-CoA: 29.2

ctg10_6

Search NORINE for peptide: strict or relaxed

AMP-binding (7..410): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ahnpFaLsvK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 30% (weak)

c00013_3300026.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 200,846 - 271,347 nt. (total: 70,502 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 200845 - 271347:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (thr) + (tyr) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg13_193

PKS_AT (534..826): mal

ATSignature: 2-Rhyd-Malonyl-CoA, Malonyl-CoA

Top 3 matches:: 2-Rhyd-Malonyl-CoA: 62.5%; Malonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 119.8
inactive: 89.5
Methoxymalonyl-CoA: 61.1
Methylmalonyl-CoA: 36.5
Ethylmalonyl-CoA: 33.1
Propionyl-CoA: 25.8
Isobutyryl-CoA: 20.9
Benzoyl-CoA: 19.2
2-Methylbutyryl-CoA: 11.8
CHC-CoA: 10.1
fatty_acid: 9.6
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ctg13_194

Search NORINE for peptide: strict or relaxed

AMP-binding (506..900): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg13_195

Search NORINE for peptide: strict or relaxed

AMP-binding (11..393): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DfFLlsyLlK
Nearest Stachelhaus code:: phg
Stachelhaus code match:: 50% (weak)

CAL_domain (603..1040): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 25.0
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

ctg13_196

Search NORINE for peptide: strict or relaxed

AMP-binding (503..909): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg13_197

PKS_AT (638..936): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; CE-Malonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 128.5
Methoxymalonyl-CoA: 113.6
Malonyl-CoA: 104.8
Ethylmalonyl-CoA: 100.7
Isobutyryl-CoA: 96.9
Propionyl-CoA: 89.0
Acetyl-CoA: 77.6
Benzoyl-CoA: 72.9
3-Methylbutyryl-CoA: 72.2
trans-1,2-CPDA: 69.3
2-Methylbutyryl-CoA: 64.2
CHC-CoA: 41.4
fatty_acid: 40.7
inactive: 40.4

ctg13_202

Search NORINE for peptide: strict or relaxed

AMP-binding (15..405): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: hcnFfGGPfK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 50% (weak)

c00014_3300026.. - Region 1 - NRPS-like

Download region GenBank file

Location: 224,156 - 247,083 nt. (total: 22,928 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 224155 - 247083:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg14_195

Search NORINE for peptide: strict or relaxed

AMP-binding (119..520): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

c00015_3300026.. - Region 1 - NRPS

Download region GenBank file

Location: 344,929 - 393,572 nt. (total: 48,644 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 344928 - 393572:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (tyr) + (asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg15_300

Search NORINE for peptide: strict or relaxed

AMP-binding (24..411): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 100% (strong)

ctg15_302

Search NORINE for peptide: strict or relaxed

AMP-binding (507..910): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIahVsK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 70% (weak)

c00015_3300026.. - Region 2 - terpene

Download region GenBank file

Location: 406,145 - 428,224 nt. (total: 22,080 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

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c00016_3300026.. - Region 1 - terpene

Download region GenBank file

Location: 167,016 - 188,026 nt. (total: 21,011 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

Legend:

core biosynthetic genes

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c00017_3300026.. - Region 1 - T3PKS

Download region GenBank file

Location: 119,476 - 160,540 nt. (total: 41,065 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

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