3300027111_7 - 14 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00002_3300027.. (original name was: 3300027111.a:Ga0209281_1000040)

Region	Type	From	To
Region 2.1	NRPS	1	62,308
Region 2.2	siderophore	103,868	115,763
Region 2.3	butyrolactone	140,289	153,705

c00006_3300027.. (original name was: 3300027111.a:Ga0209281_1000018)

Region	Type	From	To	Most similar known cluster		Similarity
Region 6.1	NRPS	259,466	312,452
Region 6.2	NAGGN	433,428	448,315

c00008_3300027.. (original name was: 3300027111.a:Ga0209281_1000011)

Region	Type	From	To
Region 8.1	NRPS	1	62,221
Region 8.2	betalactone	251,345	274,623
Region 8.3	bacteriocin	286,259	297,119
Region 8.4	T3PKS	517,279	558,328

c00011_3300027.. (original name was: 3300027111.a:Ga0209281_1000015)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	butyrolactone	58,228	69,028

c00020_3300027.. (original name was: 3300027111.a:Ga0209281_1000033)

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	arylpolyene	255,444	299,055

c00021_3300027.. (original name was: 3300027111.a:Ga0209281_1000202)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	bacteriocin	94,015	104,902

c00022_3300027.. (original name was: 3300027111.a:Ga0209281_1000249)

Region	Type	From	To	Most similar known cluster		Similarity
Region 22.1	lanthipeptide	2,944	26,015

c00024_3300027.. (original name was: 3300027111.a:Ga0209281_1000410)

Region	Type	From	To	Most similar known cluster		Similarity
Region 24.1	NRPS-like	1	38,496

Region	Type	From	To	Similarity
Region 2.1	NRPS	1	62,308
Region 2.2	siderophore	103,868	115,763
Region 2.3	butyrolactone	140,289	153,705
Region 6.1	NRPS	259,466	312,452
Region 6.2	NAGGN	433,428	448,315
Region 8.1	NRPS	1	62,221
Region 8.2	betalactone	251,345	274,623
Region 8.3	bacteriocin	286,259	297,119
Region 8.4	T3PKS	517,279	558,328
Region 11.1	butyrolactone	58,228	69,028
Region 20.1	arylpolyene	255,444	299,055
Region 21.1	bacteriocin	94,015	104,902
Region 22.1	lanthipeptide	2,944	26,015
Region 24.1	NRPS-like	1	38,496

No secondary metabolite regions were found in these records:: c00001_3300027.. (original name was: 3300027111.a:Ga0209281_1002850); c00003_3300027.. (original name was: 3300027111.a:Ga0209281_1004490); c00004_3300027.. (original name was: 3300027111.a:Ga0209281_1000212); c00005_3300027.. (original name was: 3300027111.a:Ga0209281_1000717); c00007_3300027.. (original name was: 3300027111.a:Ga0209281_1000172); c00009_3300027.. (original name was: 3300027111.a:Ga0209281_1002784); c00010_3300027.. (original name was: 3300027111.a:Ga0209281_1002271); c00012_3300027.. (original name was: 3300027111.a:Ga0209281_1006560); c00013_3300027.. (original name was: 3300027111.a:Ga0209281_1000091); c00014_3300027.. (original name was: 3300027111.a:Ga0209281_1002067); c00015_3300027.. (original name was: 3300027111.a:Ga0209281_1000009); c00016_3300027.. (original name was: 3300027111.a:Ga0209281_1000656); c00017_3300027.. (original name was: 3300027111.a:Ga0209281_1001279); c00018_3300027.. (original name was: 3300027111.a:Ga0209281_1000010); c00019_3300027.. (original name was: 3300027111.a:Ga0209281_1000127); c00023_3300027.. (original name was: 3300027111.a:Ga0209281_1000019); c00025_3300027.. (original name was: 3300027111.a:Ga0209281_1000073); c00026_3300027.. (original name was: 3300027111.a:Ga0209281_1000167)

Compact view

c00002_3300027.. - Region 1 - NRPS

Download region GenBank file

Location: 1 - 62,308 nt. (total: 62,308 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 62308:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - X - ser - D-ser) + (D-asp - X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg2_6

Search NORINE for peptide: strict or relaxed

AMP-binding (423..818): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGhVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)

ctg2_7

Search NORINE for peptide: strict or relaxed

AMP-binding (475..870): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

AMP-binding (1970..2375): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

ctg2_8

Search NORINE for peptide: strict or relaxed

AMP-binding (477..881): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

AMP-binding (1551..1952): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaeLGtVDK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 70% (weak)

AMP-binding (2611..3000): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (3643..4032): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg2_9

Search NORINE for peptide: strict or relaxed

AMP-binding (420..810): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLiAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

ctg2_11

CAL_domain (37..478): pk

minowa: NH2

Prediction, score:

NH2: 113.9
fatty_acid: 38.0
AHBA: 4.2
Acetyl-CoA: 1.7
shikimic_acid: 0.0

c00002_3300027.. - Region 2 - siderophore

Download region GenBank file

Location: 103,868 - 115,763 nt. (total: 11,896 nt) Show pHMM detection rules used

siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00002_3300027.. - Region 3 - butyrolactone

Download region GenBank file

Location: 140,289 - 153,705 nt. (total: 13,417 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00006_3300027.. - Region 1 - NRPS

Download region GenBank file

Location: 259,466 - 312,452 nt. (total: 52,987 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 259465 - 312452:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (glu - D-X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg6_272

Search NORINE for peptide: strict or relaxed

CAL_domain (41..477): pk

minowa: NH2

Prediction, score:

NH2: 178.2
Acetyl-CoA: 58.3
fatty_acid: 15.3
AHBA: 9.0
shikimic_acid: -3.0

AMP-binding (1131..1528): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFGRINK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

AMP-binding (2190..2594): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAegiGAVMK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 70% (weak)

AMP-binding (3719..4125): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeLtadDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

c00006_3300027.. - Region 2 - NAGGN

Download region GenBank file

Location: 433,428 - 448,315 nt. (total: 14,888 nt) Show pHMM detection rules used

NAGGN: ((TIGR03104 or cds(GATase_7 and Asn_synthase)) and TIGR03103 and (TIGR03106 or Peptidase_M42))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00008_3300027.. - Region 1 - NRPS

Download region GenBank file

Location: 1 - 62,221 nt. (total: 62,221 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 62221:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-ala - D-X - ala) + (gly - D-thr - ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg8_16

Search NORINE for peptide: strict or relaxed

AMP-binding (517..905): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLwNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 90% (moderate)

AMP-binding (2012..2411): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeAcGGvtK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

AMP-binding (3524..3912): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLwNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 90% (moderate)

ctg8_17

Search NORINE for peptide: strict or relaxed

AMP-binding (520..915): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1578..1983): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (3100..3498): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (4162..4560): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeAcGGvtK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

c00008_3300027.. - Region 2 - betalactone

Download region GenBank file

Location: 251,345 - 274,623 nt. (total: 23,279 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00008_3300027.. - Region 3 - bacteriocin

Download region GenBank file

Location: 286,259 - 297,119 nt. (total: 10,861 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00008_3300027.. - Region 4 - T3PKS

Download region GenBank file

Location: 517,279 - 558,328 nt. (total: 41,050 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00011_3300027.. - Region 1 - butyrolactone

Download region GenBank file

Location: 58,228 - 69,028 nt. (total: 10,801 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00020_3300027.. - Region 1 - arylpolyene

Download region GenBank file

Location: 255,444 - 299,055 nt. (total: 43,612 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00021_3300027.. - Region 1 - bacteriocin

Download region GenBank file

Location: 94,015 - 104,902 nt. (total: 10,888 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

c00022_3300027.. - Region 1 - lanthipeptide

Download region GenBank file

Location: 2,944 - 26,015 nt. (total: 23,072 nt) Show pHMM detection rules used

lanthipeptide: ((LANC_like and (Lant_dehydr_N or Lant_dehydr_C) or cds(LANC_like and (Pkinase or DUF4135))) and not (YcaO or TIGR03882))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Lanthipeptides

Lanthipeptide predictions

Lanthipeptide(s) for ctg22_10 - Putative Class II

ctg22_12 leader / core peptide: MGEHILAM - DhaGDFDGDFNAYQQVNHLGIEDhaAARDhbIVECGDhbVHCDhbAILC
allorf_006907_007074 leader / core peptide: VRASSRASPLPQGNAVHVGA - GLPAKDhbDhaVQDhbDhbDhbRDhbCVNPIRPRHDVLDhaYPFQNRLG

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg22_12 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 4063.9 Da

Molecular weight: 4066.4 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4084.5 Da N = 1; 4102.5 Da N = 2

allorf_006907_007074 - Class II

Cleavage pHMM score: -6.70

RODEO score: 0

Monoisotopic mass: 3796.1 Da

Molecular weight: 3798.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3816.3 Da N = 1; 3834.3 Da N = 2; 3852.4 Da N = 3; 3870.4 Da N = 4; 3888.4 Da N = 5; 3906.4 Da N = 6

c00024_3300027.. - Region 1 - NRPS-like

Download region GenBank file

Location: 1 - 38,496 nt. (total: 38,496 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 38496:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg24_8

Search NORINE for peptide: strict or relaxed

AMP-binding (264..609): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DALFiGiVlK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 70% (weak)