3300027309_2 - 11 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00003_3300027.. (original name was: 3300027309.a:Ga0209127_100024)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	resorcinol,arylpolyene	368,089	400,491

c00004_3300027.. (original name was: 3300027309.a:Ga0209127_100089)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS	1	27,885

c00010_3300027.. (original name was: 3300027309.a:Ga0209127_100010)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	resorcinol	361,104	403,062

c00012_3300027.. (original name was: 3300027309.a:Ga0209127_100012)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	NRPS,T1PKS	63,151	177,947
Region 12.2	arylpolyene	622,961	664,169

c00016_3300027.. (original name was: 3300027309.a:Ga0209127_100004)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	terpene	407,659	429,409
Region 16.2	hserlactone,NRPS-like	1,124,874	1,156,115

c00017_3300027.. (original name was: 3300027309.a:Ga0209127_100002)

Region	Type	From	To
Region 17.1	T1PKS,NRPS	1	38,281
Region 17.2	NRPS	233,247	280,722
Region 17.3	lassopeptide	1,153,630	1,175,809

c00019_3300027.. (original name was: 3300027309.a:Ga0209127_100061)

Region	Type	From	To	Most similar known cluster		Similarity
Region 19.1	NRPS	1	39,826

Region	Type	From	To	Similarity
Region 3.1	resorcinol,arylpolyene	368,089	400,491
Region 4.1	NRPS	1	27,885
Region 10.1	resorcinol	361,104	403,062
Region 12.1	NRPS,T1PKS	63,151	177,947
Region 12.2	arylpolyene	622,961	664,169
Region 16.1	terpene	407,659	429,409
Region 16.2	hserlactone,NRPS-like	1,124,874	1,156,115
Region 17.1	T1PKS,NRPS	1	38,281
Region 17.2	NRPS	233,247	280,722
Region 17.3	lassopeptide	1,153,630	1,175,809
Region 19.1	NRPS	1	39,826

No secondary metabolite regions were found in these records:: c00001_3300027.. (original name was: 3300027309.a:Ga0209127_100062); c00002_3300027.. (original name was: 3300027309.a:Ga0209127_100076); c00005_3300027.. (original name was: 3300027309.a:Ga0209127_100060); c00006_3300027.. (original name was: 3300027309.a:Ga0209127_100675); c00007_3300027.. (original name was: 3300027309.a:Ga0209127_100008); c00008_3300027.. (original name was: 3300027309.a:Ga0209127_100031); c00009_3300027.. (original name was: 3300027309.a:Ga0209127_100097); c00011_3300027.. (original name was: 3300027309.a:Ga0209127_100045); c00013_3300027.. (original name was: 3300027309.a:Ga0209127_100058); c00014_3300027.. (original name was: 3300027309.a:Ga0209127_100036); c00015_3300027.. (original name was: 3300027309.a:Ga0209127_100069); c00018_3300027.. (original name was: 3300027309.a:Ga0209127_100094); c00020_3300027.. (original name was: 3300027309.a:Ga0209127_100078); c00021_3300027.. (original name was: 3300027309.a:Ga0209127_100101); c00022_3300027.. (original name was: 3300027309.a:Ga0209127_100032)

Compact view

c00003_3300027.. - Region 1 - arylpolyene,resorcinol

Download region GenBank file

Location: 368,089 - 400,491 nt. (total: 32,403 nt) Show pHMM detection rules used

Region on contig edge.

resorcinol: DarB
arylpolyene: (APE_KS1 or APE_KS2)

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c00004_3300027.. - Region 1 - NRPS

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Location: 1 - 27,885 nt. (total: 27,885 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

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core biosynthetic genes

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 27885:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_1

Search NORINE for peptide: strict or relaxed

CAL_domain (41..484): pk

minowa: NH2

Prediction, score:

NH2: 21.9
AHBA: 21.2
fatty_acid: 16.6
Acetyl-CoA: 1.3
shikimic_acid: 0.0

AMP-binding (1158..1562): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2222..2628): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

c00010_3300027.. - Region 1 - resorcinol

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Location: 361,104 - 403,062 nt. (total: 41,959 nt) Show pHMM detection rules used

resorcinol: DarB

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c00012_3300027.. - Region 1 - NRPS,T1PKS

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Location: 63,151 - 177,947 nt. (total: 114,797 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 63150 - 177947:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - X) + (asp) + (pro - pro - thr) + (X) + (ccmal) + (X - X) + (X - cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg12_74

Search NORINE for peptide: strict or relaxed

AMP-binding (442..831): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLiAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

AMP-binding (1490..1888): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

ctg12_75

Search NORINE for peptide: strict or relaxed

AMP-binding (401..790): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLiAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

AMP-binding (1434..1838): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVwSgGLVyK
Nearest Stachelhaus code:: d-lyserg
Stachelhaus code match:: 70% (weak)

ctg12_81

Search NORINE for peptide: strict or relaxed

PKS_AT (526..808): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 94.6
Methoxymalonyl-CoA: 56.7
inactive: 55.3
Methylmalonyl-CoA: 47.6
2-Methylbutyryl-CoA: 35.7
Ethylmalonyl-CoA: 34.1
CHC-CoA: 32.8
Propionyl-CoA: 27.2
trans-1,2-CPDA: 26.1
Acetyl-CoA: 20.1
Isobutyryl-CoA: 19.5
Benzoyl-CoA: 19.4
fatty_acid: 13.2
3-Methylbutyryl-CoA: 10.7

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%

AMP-binding (2236..2628): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSaLiAEVwK
Nearest Stachelhaus code:: his
Stachelhaus code match:: 70% (weak)

ctg12_82

Search NORINE for peptide: strict or relaxed

CAL_domain (71..368): pk

minowa: NH2

Prediction, score:

NH2: 171.4
Acetyl-CoA: 44.4
AHBA: 19.0
shikimic_acid: 13.9
fatty_acid: 13.6

AMP-binding (1061..1455): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DlWSaGfVwK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 60% (weak)

ctg12_84

Search NORINE for peptide: strict or relaxed

AMP-binding (1176..1577): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DaytItHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 60% (weak)

AMP-binding (2235..2636): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DaytItHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 60% (weak)

AMP-binding (3292..3698): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIsMVfK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 80% (moderate)

ctg12_85

Search NORINE for peptide: strict or relaxed

AMP-binding (502..899): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg12_86

Search NORINE for peptide: strict or relaxed

AMP-binding (493..894): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWHFgLIaK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

AMP-binding (1584..1985): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWHFgLIaK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

ctg12_94

Search NORINE for peptide: strict or relaxed

AMP-binding (486..890): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgEacaGITK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 60% (weak)

c00012_3300027.. - Region 2 - arylpolyene

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Location: 622,961 - 664,169 nt. (total: 41,209 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

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c00016_3300027.. - Region 1 - terpene

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Location: 407,659 - 429,409 nt. (total: 21,751 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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c00016_3300027.. - Region 2 - NRPS-like,hserlactone

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Location: 1,124,874 - 1,156,115 nt. (total: 31,242 nt) Show pHMM detection rules used

Region on contig edge.

hserlactone: Autoind_synth
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

c00017_3300027.. - Region 1 - NRPS,T1PKS

Download region GenBank file

Location: 1 - 38,281 nt. (total: 38,281 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

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other genes

resistance

TTA codons

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 38281:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal) + (X - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg17_2

Search NORINE for peptide: strict or relaxed

AMP-binding (515..909): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvWNIGliHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

AMP-binding (1570..1967): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

ctg17_3

PKS_AT (545..837): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.3
inactive: 91.8
Methoxymalonyl-CoA: 73.5
Methylmalonyl-CoA: 66.2
Ethylmalonyl-CoA: 49.4
Propionyl-CoA: 47.0
2-Methylbutyryl-CoA: 29.9
Isobutyryl-CoA: 29.7
CHC-CoA: 24.9
Acetyl-CoA: 21.4
Benzoyl-CoA: 18.8
fatty_acid: 17.8
trans-1,2-CPDA: 11.3
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 58.3%

ctg17_4

Search NORINE for peptide: strict or relaxed

CAL_domain (49..488): pk

minowa: NH2

Prediction, score:

NH2: 179.0
Acetyl-CoA: 33.9
AHBA: 8.4
fatty_acid: 7.5
shikimic_acid: 0.0

AMP-binding (1160..1556): X

NRPSPredictor2: gly, ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: gly, ala
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DvWNIGliHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 70% (weak)

c00017_3300027.. - Region 2 - NRPS

Download region GenBank file

Location: 233,247 - 280,722 nt. (total: 47,476 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

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other genes

resistance

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 2, location 233246 - 280722:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ser - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg17_228

Search NORINE for peptide: strict or relaxed

AMP-binding (468..869): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (1531..1920): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAmlvGAICK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)

c00017_3300027.. - Region 3 - lassopeptide

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Location: 1,153,630 - 1,175,809 nt. (total: 22,180 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

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Lasso peptides

Lasso peptide predictions

Lassopeptides - No core peptides found.

Lasso peptides

Core peptide predictions

No core peptides found.

c00019_3300027.. - Region 1 - NRPS

Download region GenBank file

Location: 1 - 39,826 nt. (total: 39,826 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 39826:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (gly) + (asp) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg19_1

Search NORINE for peptide: strict or relaxed

AMP-binding (523..937): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeDhGtVtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 50% (weak)

ctg19_2

Search NORINE for peptide: strict or relaxed

AMP-binding (518..916): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DmLQLGmVWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 80% (moderate)

ctg19_3

Search NORINE for peptide: strict or relaxed

AMP-binding (464..841): asp

NRPSPredictor2: asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asp

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code:: asp
Stachelhaus code match:: 100% (strong)

ctg19_4

Search NORINE for peptide: strict or relaxed

AMP-binding (497..911): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgeDhGtVtK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 50% (weak)

AMP-binding (1581..1986): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAEyIGaVtK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 70% (weak)