3300028582_16 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00004_3300028.. (original name was: 3300028582.a:Ga0268360_1000006)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	NRPS,T1PKS,transAT-PKS	232,688	368,927

c00005_3300028.. (original name was: 3300028582.a:Ga0268360_1000047)

Region	Type	From	To	Most similar known cluster		Similarity
Region 5.1	terpene	31,335	53,022

No secondary metabolite regions were found in these records:: c00001_3300028.. (original name was: 3300028582.a:Ga0268360_1000068); c00002_3300028.. (original name was: 3300028582.a:Ga0268360_1000035); c00003_3300028.. (original name was: 3300028582.a:Ga0268360_1000192); c00006_3300028.. (original name was: 3300028582.a:Ga0268360_1004988); c00007_3300028.. (original name was: 3300028582.a:Ga0268360_1000061); c00008_3300028.. (original name was: 3300028582.a:Ga0268360_1001163); c00009_3300028.. (original name was: 3300028582.a:Ga0268360_1000098); c00010_3300028.. (original name was: 3300028582.a:Ga0268360_1000040); c00011_3300028.. (original name was: 3300028582.a:Ga0268360_1000228); c00012_3300028.. (original name was: 3300028582.a:Ga0268360_1000045); c00013_3300028.. (original name was: 3300028582.a:Ga0268360_1000052); c00014_3300028.. (original name was: 3300028582.a:Ga0268360_1000072); c00015_3300028.. (original name was: 3300028582.a:Ga0268360_1000473); c00016_3300028.. (original name was: 3300028582.a:Ga0268360_1000492)

Compact view

c00004_3300028.. - Region 1 - NRPS,T1PKS,transAT-PKS

Download region GenBank file

Location: 232,688 - 368,927 nt. (total: 136,240 nt) Show pHMM detection rules used

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
transAT-PKS: (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 232687 - 368927:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - gln) + (thr - leu - ohmal - X) + (mal) + (X) + (pro) + (X - X - glu) + (X - thr - ccmal - ala - pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 232687 - 310680:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - gln) + (thr - leu - ohmal - X) + (mal) + (X) + (pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 293957 - 368927:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - glu) + (X - thr - ccmal - ala - pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg4_185

Search NORINE for peptide: strict or relaxed

CAL_domain (37..477): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 152.3
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

AMP-binding (1137..1538): X

NRPSPredictor2: ser, thr, dhpg, hpg

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DtydSGiiGK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 50% (weak)

AMP-binding (2178..2579): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDLGmVDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

ctg4_186

Search NORINE for peptide: strict or relaxed

AMP-binding (529..915): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1587..1980): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWytGNVcK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 70% (weak)

PKS_AT (2696..2985): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; Methoxymalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.9
inactive: 59.0
Methoxymalonyl-CoA: 51.6
Methylmalonyl-CoA: 44.6
CHC-CoA: 24.6
Propionyl-CoA: 24.5
fatty_acid: 18.7
Acetyl-CoA: 17.1
Ethylmalonyl-CoA: 16.5
Benzoyl-CoA: 15.5
trans-1,2-CPDA: 10.2
2-Methylbutyryl-CoA: 9.3
Isobutyryl-CoA: 6.9
3-Methylbutyryl-CoA: 0.0

AMP-binding (4118..4528): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DmWHFSfVvK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 70% (weak)

ctg4_187

PKS_AT (525..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 74.6
inactive: 57.1
Methoxymalonyl-CoA: 38.8
Methylmalonyl-CoA: 23.9
Isobutyryl-CoA: 15.1
Propionyl-CoA: 14.2
Benzoyl-CoA: 11.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg4_188

Search NORINE for peptide: strict or relaxed

AMP-binding (534..941): X

NRPSPredictor2: asp, asn, glu, gln, aad

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DiWeItAdDK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 60% (weak)

ctg4_189

Search NORINE for peptide: strict or relaxed

AMP-binding (1121..1483): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVIK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

ctg4_208

PKS_AT (5..282): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 65.8
inactive: 37.1
Methoxymalonyl-CoA: 31.6
Methylmalonyl-CoA: 20.5
Ethylmalonyl-CoA: 19.4
Isobutyryl-CoA: 10.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ctg4_209

Search NORINE for peptide: strict or relaxed

AMP-binding (468..855): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAmTIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 90% (moderate)

AMP-binding (1507..1920): X

NRPSPredictor2: glu, gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAKDvGVdvK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 70% (weak)

AMP-binding (2574..2978): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DvkDfGfVEK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 60% (weak)

ctg4_210

Search NORINE for peptide: strict or relaxed

AMP-binding (499..897): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAmlfGVTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 70% (weak)

AMP-binding (1549..1959): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMiHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 90% (moderate)

AMP-binding (4105..4489): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

AMP-binding (5148..5548): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQtIsqVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 70% (weak)

ctg4_214

Search NORINE for peptide: strict or relaxed

AMP-binding (38..451): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: akWcvAGVaK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 50% (weak)