LOCUS       c00053_3300028..        4226 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  3300028622.a:Ga0302253_1006293.
ACCESSION   c00053_3300028..
VERSION     c00053_3300028..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 5.1.0
            Run date     :: 2019-11-23 05:08:53
            Original ID  :: 3300028622.a:Ga0302253_1006293
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4226
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..522
                     /locus_tag="ctg53_1"
                     /transl_table=11
                     /translation="VKRGTMFPMRAAKLYSLFQSCEKLEDIPPGDRTMIEKNMLLGSFE
                     DTWDLVCRYFTERDPEQIEMAERSPKHKMALLFRFYLGQASRWAISADPKRKFDYQIWC
                     GPAMGSFNEWTKGTFLEDISNRKAVTVARNLLHGAAVITRLHNLRYQGVIIPELDPAPL
                     TDSCLDNYLQ"
     protocluster    1..4226
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[536:4226](+)"
                     /cutoff="20000"
                     /detection_rule="(hglE or hglD)"
                     /neighbourhood="20000"
                     /product="hglE-KS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      537..4226
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(hglE or hglD)"
                     /neighbourhood="20000"
                     /product="hglE-KS"
                     /protocluster_number="1"
     cand_cluster    1..4226
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(hglE or hglD)"
                     /kind="single"
                     /product="hglE-KS"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4226
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="hglE-KS"
                     /region_number="1"
                     /rules="(hglE or hglD)"
                     /tool="antismash"
     CDS             537..4226
                     /NRPS_PKS="Domain: PKS_KS(Enediyne-KS) (12-466). E-value:
                     7e-122. Score: 399.1. Matches aSDomain:
                     nrpspksdomains_ctg53_2_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (577-878). E-value: 1.8e-65.
                     Score: 213.0. Matches aSDomain:
                     nrpspksdomains_ctg53_2_PKS_AT.1"
                     /NRPS_PKS="Domain: PP-binding (1145-1212). E-value: 1e-09.
                     Score: 30.5. Matches aSDomain:
                     nrpspksdomains_ctg53_2_PP-binding.1"
                     /NRPS_PKS="type: Type I Enediyne PKS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hglE-KS: hglE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 256.2; E-value:
                     9.5e-78)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg53_2"
                     /sec_met_domain="PP-binding (E-value: 4.1e-11, bitscore:
                     30.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 7e-67, bitscore: 213.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="hglE (E-value: 3.2e-181, bitscore: 589.4,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LKSEPTSSTVPLAIIGIGCLFPGADDHRQFWSNIRNGVDSISTVP
                     DTHWKLEDYFDSNQKAPDMTYGSRGGFISPVDFDPLEYAIAPNTLEAIDTSQLLGLMVA
                     KKALRDAGYGDGREFNRRNTSVILGVTGTLELVIPLGARLGHPRWRKALKDAGVSDDKM
                     EQVISRISESYVEWQESSFPGLLGNVVAGRIANYFDLGGTNCVVDAACASSLSALHLAT
                     LELSSGKSDMVLAGGVDTFNDIFMFMCFSKTPALSPTGDARPFAHNCDGTILGEGLGMV
                     VMKRLADAERDGDRIYAVIKGIGSSSDGKGAAVFAPQSDGQIRAMQNAYRCTGISPDTV
                     ELMEAHGTGTRVGDQTEITSLCTVYQQAGGEHGWCALGSVKSQIGHTKAAAGIAGLIKS
                     ALSLYHKVLPPTIKVEKPMDELISEDTPFYINTEKRPWIRRSDMPRRAALSSFGFGGSN
                     FHCILEESKDQKKPADWDDTVQILAFSAATPEELKSELGKRPDSLGGCDLAAYAEKTRS
                     TFRPDAHCRLIAVVEKDHHDAVKIIDSAASMLDKNAGKDFWTTPDGVFYGAGEKPGKLG
                     VLFPGQGAQYTGMLRDLACQFPEMHDVLSEADRSFERNKAVNLKERLSGYIYPVPSFSA
                     DERENREAALRDTRVAQPALGAVDLGAFRVLQYFGVRSDAFAGHSYGELVALAAAGTIG
                     TADLHKLSAMRGNLMTGSPGDLGSMVAVLADMDTVKKCVSEHSLNVVTANVNAPSQIVL
                     SGATDEIRRASQILTEIGIKNTVLHVSAAFHSPLVADAQAPFAEALKNIEFSKAVNPVF
                     SNTTGQRYPDDPAEARSLLAGQLALPVQFVDEIRAMYEYGVRTFIEAGPGSRMTGLVKS
                     ILGDAGDCRTMSLDSSAGKRSGTSDLARMLAFLAASGHDADLTLWNPATPQQKADWAKP
                     KPRMVVKLCGANYVKPSKARSAAAEQKPERQMNVSVQRPQPLQAPAASLSIEKQSTMEL
                     SGTAERAGQNNQQAIRRQTQSSTTVPDAGDGSLLLEALRVTQENMRSLQSIQEQTAKMH
                     MHFLQNQDTALKTFQELAEQQQLLFQQSLGLASSGYQAPALRREIASDRTAVAASPAAP
                     AHAPEPVPQHSLRSESAPAQAQPAGHHSAPEADSAAVSEKLISIVSEKTGYPAEMLELS
                     MSLDADLGIDSIKRVEIFSALREAIPELPTFKPDQMNSLKTLGDITSFMASSMPASGSA
                     PQAAK"
     aSDomain        573..1934
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg53_2_PKS_KS.1"
                     /evalue="7.00E-122"
                     /label="ctg53_2_PKS_KS.1"
                     /locus_tag="ctg53_2"
                     /protein_end="466"
                     /protein_start="12"
                     /score="399.1"
                     /tool="antismash"
                     /translation="AIIGIGCLFPGADDHRQFWSNIRNGVDSISTVPDTHWKLEDYFDS
                     NQKAPDMTYGSRGGFISPVDFDPLEYAIAPNTLEAIDTSQLLGLMVAKKALRDAGYGDG
                     REFNRRNTSVILGVTGTLELVIPLGARLGHPRWRKALKDAGVSDDKMEQVISRISESYV
                     EWQESSFPGLLGNVVAGRIANYFDLGGTNCVVDAACASSLSALHLATLELSSGKSDMVL
                     AGGVDTFNDIFMFMCFSKTPALSPTGDARPFAHNCDGTILGEGLGMVVMKRLADAERDG
                     DRIYAVIKGIGSSSDGKGAAVFAPQSDGQIRAMQNAYRCTGISPDTVELMEAHGTGTRV
                     GDQTEITSLCTVYQQAGGEHGWCALGSVKSQIGHTKAAAGIAGLIKSALSLYHKVLPPT
                     IKVEKPMDELISEDTPFYINTEKRPWIRRSDMPRRAALSSFGFGGSNFHCILEESK"
     aSModule        573..4172
                     /complete
                     /domains="nrpspksdomains_ctg53_2_PKS_KS.1"
                     /domains="nrpspksdomains_ctg53_2_PKS_AT.1"
                     /domains="nrpspksdomains_ctg53_2_PP-binding.1"
                     /locus_tags="ctg53_2"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1140..1187
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg53_2_0001"
                     /evalue="1.10E-02"
                     /label="PKSI-KS_m3"
                     /locus_tag="ctg53_2"
                     /protein_end="217"
                     /protein_start="201"
                     /score="8.7"
                     /tool="antismash"
                     /translation="GTNCVVDAACASSLSAL"
     CDS_motif       1563..1607
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg53_2_0002"
                     /evalue="9.60E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ctg53_2"
                     /protein_end="357"
                     /protein_start="342"
                     /score="11.6"
                     /tool="antismash"
                     /translation="MEAHGTGTRVGDQTEI"
     CDS_motif       1668..1712
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg53_2_0003"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="ctg53_2"
                     /protein_end="392"
                     /protein_start="377"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSVKSQIGHTKAAAGI"
     CDS_motif       2268..2315
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg53_2_0004"
                     /evalue="6.40E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="ctg53_2"
                     /protein_end="593"
                     /protein_start="577"
                     /score="15.5"
                     /tool="antismash"
                     /translation="LFPGQGAQYTGMLRDLA"
     aSDomain        2268..3170
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg53_2_PKS_AT.1"
                     /evalue="1.80E-65"
                     /label="ctg53_2_PKS_AT.1"
                     /locus_tag="ctg53_2"
                     /protein_end="878"
                     /protein_start="577"
                     /score="213.0"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="consensus: mal"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFPGQGAQYTGMLRDLACQFPEMHDVLSEADRSFERNKAVNLKER
                     LSGYIYPVPSFSADERENREAALRDTRVAQPALGAVDLGAFRVLQYFGVRSDAFAGHSY
                     GELVALAAAGTIGTADLHKLSAMRGNLMTGSPGDLGSMVAVLADMDTVKKCVSEHSLNV
                     VTANVNAPSQIVLSGATDEIRRASQILTEIGIKNTVLHVSAAFHSPLVADAQAPFAEAL
                     KNIEFSKAVNPVFSNTTGQRYPDDPAEARSLLAGQLALPVQFVDEIRAMYEYGVRTFIE
                     AGPGSRMTGLVKSILGDAGDC"
     CDS_motif       2544..2663
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg53_2_0005"
                     /evalue="5.80E-07"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="ctg53_2"
                     /protein_end="709"
                     /protein_start="669"
                     /score="21.4"
                     /tool="antismash"
                     /translation="GVRSDAFAGHSYGELVALAAAGTIGTADLHKLSAMRGNLMT"
     aSDomain        3972..4172
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg53_2_PP-binding.1"
                     /evalue="1.00E-09"
                     /label="ctg53_2_PP-binding.1"
                     /locus_tag="ctg53_2"
                     /protein_end="1212"
                     /protein_start="1145"
                     /score="30.5"
                     /tool="antismash"
                     /translation="EKLISIVSEKTGYPAEMLELSMSLDADLGIDSIKRVEIFSALREA
                     IPELPTFKPDQMNSLKTLGDITS"
ORIGIN
        1 gtcaagagag gcaccatgtt cccgatgaga gccgcaaagc tctactcact tttccagagc
       61 tgtgaaaagc tggaggatat tccacccggt gatagaacca tgatcgaaaa aaatatgctc
      121 ctcggctcat ttgaagatac gtgggacctg gtgtgcaggt atttcacgga aagagacccg
      181 gagcagatag agatggcaga gagaagtccg aagcacaaaa tggcgctgct tttcagattt
      241 tatctgggcc aggcctcccg gtgggccatc tcggcagatc cgaagagaaa gttcgattat
      301 cagatctggt gcgggcctgc aatgggctcg ttcaatgaat ggacgaaggg aaccttcctt
      361 gaggacatat cgaaccggaa ggccgtgaca gtggccagaa acctgctcca cggagcggcg
      421 gtgataacaa ggctccataa tctgagatat cagggcgtca ttattccgga gcttgatccg
      481 gcacctctca ctgattcttg tctcgacaac tacttgcagt aaagaaggcg atatctttga
      541 aatccgaacc cacctcatca acggtcccgc tggcaataat cggcatcgga tgccttttcc
      601 ccggcgcaga cgatcacagg cagttctggt ccaatataag gaatggagtg gattccatct
      661 caacggttcc tgacactcac tggaagctcg aagactattt cgattccaac cagaaggcgc
      721 ctgatatgac ttacggctcg cggggaggat tcatatcccc tgtcgatttc gatccactcg
      781 aatacgcaat cgccccgaac actctggagg caatagacac atcccagctg ctggggctca
      841 tggtagccaa aaaggctctt cgcgacgcag ggtacggcga tggaagggag ttcaaccgca
      901 ggaacaccag tgtaatcctc ggagtcacag gcacgctcga gctggtgatc cctctggggg
      961 ctaggctcgg ccaccccaga tggagaaagg ccctcaaaga tgccggtgtc agcgatgaca
     1021 agatggagca ggtcatcagc agaatctccg aatcgtacgt cgagtggcag gagagctcgt
     1081 tcccaggcct tctgggaaat gttgtggcag gaaggatagc aaactacttc gatctcggcg
     1141 gcacaaactg cgtggtcgac gcggcttgcg cgagctcgct ttcggcattg catctggcaa
     1201 ctctcgagct ctcgtccgga aagagcgaca tggtgctggc cggtggagtc gacacgttca
     1261 atgatatatt catgttcatg tgcttcagca agacgccggc cctctctccg acaggcgacg
     1321 cccgcccgtt cgcccacaac tgtgacggca caatcctcgg cgagggcctg ggcatggtag
     1381 tgatgaagcg gctcgccgac gccgagcggg acggcgacag gatatacgcc gtcataaagg
     1441 ggataggatc atcgagcgac ggcaaagggg ccgctgtctt cgcgccccag tccgacggcc
     1501 agatccgggc gatgcagaat gcatatcggt gcacaggcat cagccccgac accgttgagc
     1561 tgatggaagc ccacggaaca ggcaccagag tgggagacca gacagagatc acctctctct
     1621 gcacagtcta tcagcaggca ggcggagagc atggctggtg cgcgctcggc tcggtgaaat
     1681 cgcagatcgg gcacacaaag gctgccgcag gaatagcagg gctgatcaag tcggcgctct
     1741 cgctgtatca caaggttctt cctcccacga tcaaggtgga gaagccgatg gacgagctga
     1801 tatcagaaga cacgcctttc tacatcaaca ctgagaagag gccctggata aggcgctctg
     1861 acatgccgag gagggctgcc ctcagctcct tcggattcgg cggcagcaac ttccactgca
     1921 tcctcgagga atcgaaggat cagaagaagc ctgcagactg ggacgataca gttcagatcc
     1981 tggccttctc cgccgcgact ccggaagagc tcaaatccga gctcggaaag agaccggatt
     2041 cgctgggcgg ctgcgatctg gcggcttacg cagagaaaac cagaagcaca ttcaggcctg
     2101 acgcgcactg caggttgatc gccgtagtgg aaaaggacca ccacgatgct gtgaagatta
     2161 tcgacagcgc tgcttcgatg ctcgataaaa atgccggaaa agatttctgg accacgccgg
     2221 atggagtctt ctacggagcc ggagaaaagc ccggaaagct cggcgtgctg tttccgggac
     2281 agggcgcaca gtataccggc atgctgaggg acctggcctg ccagttccct gagatgcacg
     2341 atgtgctgtc ggaagcggac cgctcgttcg agagaaacaa ggccgttaat ttgaaagagc
     2401 gcctctcagg ctatatatat cctgttccat cgttcagtgc tgatgagagg gagaatcgcg
     2461 aagccgccct ccgcgacacg agagtggccc agcctgctct cggggctgtc gatctgggag
     2521 ccttcagagt gcttcagtac ttcggcgtca gatcagacgc atttgccggg cacagctacg
     2581 gcgagctggt ggctctcgcg gcagcaggca ccatcggaac ggccgatctg cacaagctgt
     2641 cagctatgag gggaaacctc atgacaggct cgcccggcga tctgggctcg atggtggcag
     2701 ttctggccga tatggacact gtcaaaaagt gcgtcagcga acattcgctg aacgtagtga
     2761 cggctaatgt caatgcgccg tctcagattg ttctgtcagg agcgaccgat gagatcaggc
     2821 gcgcttcgca gatattgacg gagatcggaa tcaagaacac ggtgctgcat gtatcagcgg
     2881 cgtttcacag ccctctcgtg gccgatgcgc aggctccatt tgccgaagcg ctgaagaata
     2941 ttgagttcag caaggcggtt aaccctgtct tttcgaacac aactggacag agataccctg
     3001 atgacccggc agaggcgcgc tcgctgctgg ccggccagct cgcccttcca gtccagttcg
     3061 tggacgagat cagggccatg tatgaatacg gagtgagaac tttcatcgag gcaggtcccg
     3121 gctccaggat gacaggcctg gtcaaatcga tactcggcga cgcaggggac tgcaggacca
     3181 tgtcgctcga ttcatcggca gggaagcgct caggaacatc ggacctggca aggatgctgg
     3241 ccttcctggc cgcatcgggc cacgatgccg atctgacgct ctggaaccct gcaacgcccc
     3301 agcagaaggc agactgggcg aagccgaagc cgcgtatggt tgtaaagctg tgcggggcga
     3361 actatgtcaa gccttcaaaa gcgcgaagcg ccgccgcaga gcagaagcct gaacggcaga
     3421 tgaacgtttc cgtgcagagg ccgcagccat tgcaggcacc tgcagcgagc ctgagtattg
     3481 aaaaacaatc aacaatggag ctctcaggca ctgcagagag ggccggacag aataatcagc
     3541 aggcgatcag aaggcagact cagagctcga cgacagttcc agacgccggc gacggctcgc
     3601 ttcttctcga ggctctcagg gtgacccagg agaacatgag atcgctgcag agcattcagg
     3661 agcagacggc gaagatgcac atgcacttcc tgcagaacca ggacacggcc ctgaagacct
     3721 tccaggagct ggccgagcag cagcagctgc ttttccagca gtccctggga cttgcatcat
     3781 caggatatca ggcaccggcc ttgagaagag agatcgcttc tgaccgcacg gctgttgcag
     3841 cctctcctgc cgcaccggca catgctccgg aaccggtacc tcagcactct ctgcgatcag
     3901 aatcggcacc agcgcaggcg cagcctgccg gtcatcattc agcaccggag gccgactcgg
     3961 ctgcagtatc tgaaaagctt atttcgatcg tatccgaaaa gacaggctat cccgccgaga
     4021 tgctcgagct ctcgatgagc ctcgatgctg atctcggaat agactcgata aagcgggtcg
     4081 agatcttctc ggctctcaga gaggcgatac ctgagcttcc gacattcaag ccagatcaga
     4141 tgaactcgct gaagactctc ggagacataa cgtctttcat ggcgagctca atgccggcct
     4201 ctggcagtgc cccccaggcg gcaaag
//
