3300028923_3 - 8 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

c00018_3300028.. (original name was: 3300028923.a:Ga0310375_1000072)

Region	Type	From	To	Most similar known cluster		Similarity
Region 18.1	hserlactone	63,929	84,522

c00021_3300028.. (original name was: 3300028923.a:Ga0310375_1000083)

Region	Type	From	To	Most similar known cluster		Similarity
Region 21.1	T1PKS,NRPS	18,837	124,142

c00029_3300028.. (original name was: 3300028923.a:Ga0310375_1000158)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	NRPS	1	46,795

c00039_3300028.. (original name was: 3300028923.a:Ga0310375_1000076)

Region	Type	From	To	Most similar known cluster		Similarity
Region 39.1	bacteriocin	38,481	49,290
Region 39.2	hglE-KS	114,502	156,424

c00040_3300028.. (original name was: 3300028923.a:Ga0310375_1000033)

Region	Type	From	To	Most similar known cluster		Similarity
Region 40.1	sactipeptide,NRPS	136,596	212,377

c00042_3300028.. (original name was: 3300028923.a:Ga0310375_1000038)

Region	Type	From	To	Most similar known cluster		Similarity
Region 42.1	hglE-KS,T1PKS	239,839	293,607

c00064_3300028.. (original name was: 3300028923.a:Ga0310375_1000183)

Region	Type	From	To	Most similar known cluster		Similarity
Region 64.1	NRPS-like	1	27,388

Region	Type	From	To	Similarity
Region 18.1	hserlactone	63,929	84,522
Region 21.1	T1PKS,NRPS	18,837	124,142
Region 29.1	NRPS	1	46,795
Region 39.1	bacteriocin	38,481	49,290
Region 39.2	hglE-KS	114,502	156,424
Region 40.1	sactipeptide,NRPS	136,596	212,377
Region 42.1	hglE-KS,T1PKS	239,839	293,607
Region 64.1	NRPS-like	1	27,388

No secondary metabolite regions were found in these records:: c00001_3300028.. (original name was: 3300028923.a:Ga0310375_1000784); c00002_3300028.. (original name was: 3300028923.a:Ga0310375_1000551); c00003_3300028.. (original name was: 3300028923.a:Ga0310375_1000222); c00004_3300028.. (original name was: 3300028923.a:Ga0310375_1000157); c00005_3300028.. (original name was: 3300028923.a:Ga0310375_1000140); c00006_3300028.. (original name was: 3300028923.a:Ga0310375_1000049); c00007_3300028.. (original name was: 3300028923.a:Ga0310375_1000993); c00008_3300028.. (original name was: 3300028923.a:Ga0310375_1000020); c00009_3300028.. (original name was: 3300028923.a:Ga0310375_1000726); c00010_3300028.. (original name was: 3300028923.a:Ga0310375_1000276); c00011_3300028.. (original name was: 3300028923.a:Ga0310375_1000077); c00012_3300028.. (original name was: 3300028923.a:Ga0310375_1000034); c00013_3300028.. (original name was: 3300028923.a:Ga0310375_1000141); c00014_3300028.. (original name was: 3300028923.a:Ga0310375_1000503); c00015_3300028.. (original name was: 3300028923.a:Ga0310375_1000475); c00016_3300028.. (original name was: 3300028923.a:Ga0310375_1000401); c00017_3300028.. (original name was: 3300028923.a:Ga0310375_1000165); c00019_3300028.. (original name was: 3300028923.a:Ga0310375_1000198); c00020_3300028.. (original name was: 3300028923.a:Ga0310375_1000102); c00022_3300028.. (original name was: 3300028923.a:Ga0310375_1001197); c00023_3300028.. (original name was: 3300028923.a:Ga0310375_1000097); c00024_3300028.. (original name was: 3300028923.a:Ga0310375_1000075); c00025_3300028.. (original name was: 3300028923.a:Ga0310375_1001407); c00026_3300028.. (original name was: 3300028923.a:Ga0310375_1001997); c00027_3300028.. (original name was: 3300028923.a:Ga0310375_1000138); c00028_3300028.. (original name was: 3300028923.a:Ga0310375_1000115); c00030_3300028.. (original name was: 3300028923.a:Ga0310375_1000067); c00031_3300028.. (original name was: 3300028923.a:Ga0310375_1000053); c00032_3300028.. (original name was: 3300028923.a:Ga0310375_1000317); c00033_3300028.. (original name was: 3300028923.a:Ga0310375_1000080); c00034_3300028.. (original name was: 3300028923.a:Ga0310375_1000087); c00035_3300028.. (original name was: 3300028923.a:Ga0310375_1000069); c00036_3300028.. (original name was: 3300028923.a:Ga0310375_1000319); c00037_3300028.. (original name was: 3300028923.a:Ga0310375_1000021); c00038_3300028.. (original name was: 3300028923.a:Ga0310375_1000035); c00041_3300028.. (original name was: 3300028923.a:Ga0310375_1000056); c00043_3300028.. (original name was: 3300028923.a:Ga0310375_1000161); c00044_3300028.. (original name was: 3300028923.a:Ga0310375_1000064); c00045_3300028.. (original name was: 3300028923.a:Ga0310375_1000071); c00046_3300028.. (original name was: 3300028923.a:Ga0310375_1000095); c00047_3300028.. (original name was: 3300028923.a:Ga0310375_1000084); c00048_3300028.. (original name was: 3300028923.a:Ga0310375_1000111); c00049_3300028.. (original name was: 3300028923.a:Ga0310375_1000237); c00050_3300028.. (original name was: 3300028923.a:Ga0310375_1000032); c00051_3300028.. (original name was: 3300028923.a:Ga0310375_1000391); c00052_3300028.. (original name was: 3300028923.a:Ga0310375_1000014); c00053_3300028.. (original name was: 3300028923.a:Ga0310375_1000623); c00054_3300028.. (original name was: 3300028923.a:Ga0310375_1002364); c00055_3300028.. (original name was: 3300028923.a:Ga0310375_1004692); c00056_3300028.. (original name was: 3300028923.a:Ga0310375_1000073); c00057_3300028.. (original name was: 3300028923.a:Ga0310375_1000100); c00058_3300028.. (original name was: 3300028923.a:Ga0310375_1000105); c00059_3300028.. (original name was: 3300028923.a:Ga0310375_1000225); c00060_3300028.. (original name was: 3300028923.a:Ga0310375_1000423); c00061_3300028.. (original name was: 3300028923.a:Ga0310375_1000294); c00062_3300028.. (original name was: 3300028923.a:Ga0310375_1000612); c00063_3300028.. (original name was: 3300028923.a:Ga0310375_1003926); c00065_3300028.. (original name was: 3300028923.a:Ga0310375_1000055); c00066_3300028.. (original name was: 3300028923.a:Ga0310375_1000113); c00067_3300028.. (original name was: 3300028923.a:Ga0310375_1000125); c00068_3300028.. (original name was: 3300028923.a:Ga0310375_1000149)

Compact view

c00018_3300028.. - Region 1 - hserlactone

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Location: 63,929 - 84,522 nt. (total: 20,594 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

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core biosynthetic genes

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Gene details

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c00021_3300028.. - Region 1 - NRPS,T1PKS

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Location: 18,837 - 124,142 nt. (total: 105,306 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

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Gene details

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NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 18836 - 124142:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (mal - ser - D-ser - ser - pro - thr - ser) + (X - D-val - thr - ile - thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg21_28

PKS_AT (16..311): mmal

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 52.2
Methoxymalonyl-CoA: 47.0
Malonyl-CoA: 20.0
Isobutyryl-CoA: 18.7
Propionyl-CoA: 15.6
fatty_acid: 14.8
CHC-CoA: 14.6
2-Methylbutyryl-CoA: 14.6
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 5.3
inactive: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.1

ATSignature: (unknown)

No matches above 50%

PKS_AT (917..1213): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.9
inactive: 123.8
Methylmalonyl-CoA: 53.5
Methoxymalonyl-CoA: 52.5
Isobutyryl-CoA: 30.7
Propionyl-CoA: 28.2
Benzoyl-CoA: 21.3
2-Methylbutyryl-CoA: 15.4
Ethylmalonyl-CoA: 14.8
Acetyl-CoA: 11.7
CHC-CoA: 10.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

ctg21_29

Search NORINE for peptide: strict or relaxed

PKS_AT (609..931): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.5
inactive: 145.0
Methoxymalonyl-CoA: 73.5
Methylmalonyl-CoA: 64.4
Propionyl-CoA: 48.1
Isobutyryl-CoA: 44.3
Benzoyl-CoA: 30.5
Ethylmalonyl-CoA: 29.4
2-Methylbutyryl-CoA: 25.3
Acetyl-CoA: 18.0
CHC-CoA: 15.4
fatty_acid: 14.0
3-Methylbutyryl-CoA: 10.6
trans-1,2-CPDA: 9.5

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

AMP-binding (2111..2506): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (3181..3579): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (4706..5113): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: gly, ala
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVsdLAiiYK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 60% (weak)

AMP-binding (5774..6179): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQYIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (6838..7245): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (8315..8714): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

ctg21_37

Search NORINE for peptide: strict or relaxed

AMP-binding (516..926): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DItnLGMIiK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 70% (weak)

AMP-binding (1586..1988): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAlLIGAVaK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

AMP-binding (4172..4473): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWsIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 90% (moderate)

AMP-binding (5132..5547): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

AMP-binding (6630..7035): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

c00029_3300028.. - Region 1 - NRPS

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Location: 1 - 46,795 nt. (total: 46,795 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

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regulatory genes

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 46795:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ala - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg29_12

Search NORINE for peptide: strict or relaxed

AMP-binding (464..848): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: pro
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DVmFdAGVLK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 80% (moderate)

AMP-binding (1490..1874): X

NRPSPredictor2: hydrophilic

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAahVGEVaK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 70% (weak)

c00039_3300028.. - Region 1 - bacteriocin

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Location: 38,481 - 49,290 nt. (total: 10,810 nt) Show pHMM detection rules used

bacteriocin: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03603 or TIGR03604 or TIGR03651 or TIGR03678 or TIGR03693 or TIGR03798 or TIGR03601 or TIGR03602 or TIGR03795 or TIGR03793 or TIGR03975 or DUF692 or TIGR01193)

Legend:

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c00039_3300028.. - Region 2 - hglE-KS

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Location: 114,502 - 156,424 nt. (total: 41,923 nt) Show pHMM detection rules used

Region on contig edge.

hglE-KS: (hglE or hglD)

Legend:

core biosynthetic genes

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c00040_3300028.. - Region 1 - NRPS,sactipeptide

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Location: 136,596 - 212,377 nt. (total: 75,782 nt) Show pHMM detection rules used

sactipeptide: (subtilosin or thuricin or TIGR04404 or TIGR03973)
NRPS: (cds(Condensation and (AMP-binding or A-OX)) or (Condensation and AMP-binding))

Legend:

core biosynthetic genes

additional biosynthetic genes

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Gene details

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NRPS/PKS domains

Sactipeptides

Detailed domain annotation

Selected features only

Show module domains

Sactipeptide predictions

Sactipeptides - No core peptides found.

NRPS/PKS products

NRPS/PKS monomers

Sactipeptides

Predicted core structure(s)

For candidate cluster 1, location 136595 - 212377:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - tyr) + (asn - D-tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 156394 - 212377:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - tyr) + (asn - D-tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg40_152

Search NORINE for peptide: strict or relaxed

AMP-binding (536..941): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKfGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 90% (moderate)

AMP-binding (1585..1992): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASdIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 90% (moderate)

ctg40_153

Search NORINE for peptide: strict or relaxed

AMP-binding (468..877): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAtiiGAICK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 70% (weak)

AMP-binding (1521..1928): tyr

NRPSPredictor2: tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASdIAAVCK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 90% (moderate)

Core peptide predictions

No core peptides found.

c00042_3300028.. - Region 1 - T1PKS,hglE-KS

Download region GenBank file

Location: 239,839 - 293,607 nt. (total: 53,769 nt) Show pHMM detection rules used

Region on contig edge.

hglE-KS: (hglE or hglD)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

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transport-related genes

regulatory genes

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 239838 - 293607:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctg42_221

PKS_AT (577..882): mal

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 61.9
Methoxymalonyl-CoA: 33.3
Methylmalonyl-CoA: 24.3
inactive: 21.8
Propionyl-CoA: 18.2
fatty_acid: 11.9
CHC-CoA: 10.3
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 62.5%

ctg42_226

PKS_AT (1061..1359): mxmal

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 17.1
Methylmalonyl-CoA: 16.0
Propionyl-CoA: 15.6
Isobutyryl-CoA: 13.5
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ATSignature: (unknown)

No matches above 50%

c00064_3300028.. - Region 1 - NRPS-like

Download region GenBank file

Location: 1 - 27,388 nt. (total: 27,388 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

NRPS/PKS domains

Detailed domain annotation

Selected features only

Show module domains