LOCUS       New Construct      1259 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1259
                     /gene="Sse9I-AATT"
                     /codon_start=1
                     /translation="MIKYLGSKRTLVPVLGDIASASGATEAVDLFTGTTRVAQEFKRR
                     GLRVLANDVATYSEVLAQCYIATNGQEVDRHALEAALAELNALPGEPGYFTETFCEAS
                     RYFQPKNGARVDAIRNAIDDRYADSWMRPILLTSLMLAADRVDSTTGVQMAYLKQWAA
                     RAHNDLELRLPDLIAGDGDVAREDAVTLAQELPRVQLMYLDPPYNQHRYFTNYHIWET
                     LIRWDAPESYGIACKRIDSRDDATKSPYNMKRRMPGEMRRLLMTIKADLAVVSYNNES
                     WIDPETMMSTLRDAGYEDVRLLAFDYKRYVGAQIGIYNPSGEKVGRVSHLRNIEYLFL
                     AGPTERVEVCAARVEHRALPKEPELTLF*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgattaagt
      181 acctgggatc gaaacgtaca ttagttccgg tcctgggcga tatagcgtct gcaagcggtg
      241 caaccgaagc ggttgatctg tttaccggta ccacacgtgt agcgcaggaa ttcaaacgtc
      301 gcggtctgcg cgtactggct aatgacgtag caacgtatag cgaagtgttg gcccagtgct
      361 atattgctac taatggacag gaagttgacc ggcatgcctt ggaagcggcg cttgctgaat
      421 tgaatgcact tccgggcgaa ccggggtatt ttacagaaac tttctgcgag gctagtcgct
      481 attttcaacc taaaaatggc gcgcgcgtgg acgcaatccg taatgctatc gacgaccgtt
      541 atgccgactc ctggatgcgc ccgattctgt taacttcact tatgctggcg gcagaccgcg
      601 tcgattcgac tacaggggtc cagatggcat atctgaaaca atgggcggca cgtgcccaca
      661 atgatttaga gctgcgcctt cccgacctga ttgccggtga cggcgatgtt gcccgtgagg
      721 atgcagttac attggcacag gaactgccgc gggttcagtt gatgtattta gatccacctt
      781 acaatcagca tcggtacttc acaaactacc acatttggga gactctgata cgttgggatg
      841 cgccagaatc ctatggcatt gcgtgtaaac gcatagattc gcgcgatgat gccaccaaat
      901 ctccatataa catgaaacgt cgcatgccag gtgaaatgcg ccgtctgctg atgaccatca
      961 aagcggattt agcggttgtt tcgtataata atgaatcatg gatagacccg gagacaatga
     1021 tgtctacgct gcgggatgcg ggctatgaag acgttcgctt attggccttt gattataagc
     1081 gctatgttgg cgcacagatc ggcatctata atccttcggg cgaaaaagtg ggtcgtgtga
     1141 gccatctgcg caatatcgag tatctgttct tggcgggtcc caccgaaaga gttgaggtct
     1201 gcgcagctag agttgagcat agagcattac cgaaggaacc agaacttaca cttttttaa
//
