LOCUS       New Construct      1736 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1736
                     /gene="M.AluI-AGCT"
                     /codon_start=1
                     /translation="MSKANAKYSFVDLFAGIGGFHAALAATGGVCEYAVEIDREAAAV
                     YERNWNKPALGDITDDANDEGVTLRGYDGPIDVLTGGFPCQPFSKSGAQHGMAETRGT
                     LFWNIARIIEEREPTVLILENVRNLVGPRHRHEWLTIIETLRFFGYEVSGAPAIFSPH
                     LLPAWMGGTPQVRERVFITATLVPERMRDERIPRTETGEIDAEAIGPKPVATMNDRFP
                     IKKGGTELFHPGDRKSGWNLLTSGIIREGDPEPSNVDLRLTETETLWIDAWDDLESTI
                     RRATGRPLEGFPYWADSWTDFRELSRLVVIRGFQAPEREVVGDRKRYVARTDMPEGFV
                     PASVTRPAIDETLPAWKQSHLRRNYDFFERHFAEVVAWAYRWGVYTDLFPASRRKLEW
                     QAQDAPRLWDTVMHFRPSGIRAKRPTYLPALVAITQTSIVGPLERRLSPRETARLQGL
                     PEWFDFGEQRAAATYKQMGNGVNVGVVRHILREHVRRDRALLKLTPAGQRIINAVLAD
                     EPDATVGALGAAE*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgagcaaag
      181 caaacgccaa gtactcattc gttgacttgt tcgccggcat cgggggtttc catgccgcgc
      241 ttgcagcaac cgggggagtg tgtgaatatg ccgtggaaat agatcgtgag gctgcagcag
      301 tttacgagag aaactggaat aaaccggccc tgggcgatat tactgacgat gccaatgacg
      361 agggagttac tctgcgtggg tacgacggtc ctatcgacgt cttaacggga ggatttccat
      421 gccaaccgtt tagcaaatca ggtgcccagc atggaatggc agagacacgc ggtaccctgt
      481 tctggaatat tgctcgcatt attgaggaac gtgaacccac agtcttaatt ttagagaatg
      541 tgcgcaattt agtaggccct agacatcgtc atgagtggtt gaccattata gagacgttaa
      601 gatttttcgg gtacgaggtt tctggagccc cggcgatttt cagtccgcat ctgcttcccg
      661 cctggatggg gggaacccca caggttagag agagagtttt tattacagct acacttgttc
      721 cggaaagaat gagagacgaa cgcattccgc gtaccgagac aggggagata gatgctgaag
      781 caatcgggcc aaaaccagtc gccaccatga atgaccggtt ccctattaaa aagggcggta
      841 ccgagctgtt ccaccccgga gatcgtaaat ccggatggaa cctgcttacg tcaggtatca
      901 tccgcgaagg agacccggaa ccttcgaacg tggacctgcg gctgaccgaa acggagaccc
      961 tgtggattga tgcctgggat gatctggaat ccacaattag acgggctacc ggccgcccct
     1021 tagagggatt tccttattgg gcggactcct ggactgattt tcgtgaatta agccggcttg
     1081 ttgtaatacg cggctttcaa gcgcctgaac gggaagtggt gggtgataga aaacgttacg
     1141 tggcacggac cgacatgccg gagggatttg taccggcgag cgtaacacgg ccggccattg
     1201 atgagacgct tcccgcgtgg aaacagagcc accttcgcag aaactacgat ttttttgagc
     1261 gtcattttgc tgaagtagtt gcttgggcgt atcgctgggg agtctatacc gacctttttc
     1321 ctgcttcgcg gcgcaaatta gaatggcagg cccaagacgc acctcgttta tgggatacgg
     1381 ttatgcattt tagaccgtcg ggaatacgcg cgaaacgtcc gacttacctt cctgctttag
     1441 tggccattac gcagacttcc attgttggcc cactggaacg gcgtctgtct ccacgcgaga
     1501 cagcccgtct gcagggactt cctgaatggt tcgatttcgg tgagcagaga gcagcggcca
     1561 cgtataaaca gatgggcaat ggcgtcaatg tgggtgttgt tcggcacatc cttcgtgagc
     1621 acgtacgtcg tgaccgtgca ctgctgaaac ttaccccagc cggccagcgc atcatcaacg
     1681 cggttctggc agatgaacct gatgcgaccg tgggcgcatt aggtgcggcg gagtaa
//
