LOCUS       New Construct      1160 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1160
                     /gene="M.HpyI-CATG"
                     /codon_start=1
                     /translation="MNYIGSKYKLIPFIKENIHAVVGHDLSGAIFCDLFAGTGIVGRA
                     FKKAVNKVISNDLEYYSFVLNQNYIGNIQEIPNQEELIDRLNSVALRKGFIHSHYSLG
                     GSSRQYFSETNAQKIDAVRLKIEELKLSQNIDNCAYYFLLASLLESADKVANTASVYG
                     AFLKRLKKSAQKELILKGAHFDLSLNANEVYQQDASELIGKISGDILYLDPPYNARQY
                     GANYHLLNTIAAYTPFTPKGKTGLPSYQKSSFCSRSQILNAFENLIKKARFKYIFLSY
                     NNEGLMSEIEIKNILKKYGAYSLMTKTYMRFKADNKRAHKAAHTKECLHILIK*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaattaca
      181 tcgggtctaa atataagctg attcctttca taaaggaaaa tatccacgcc gtggttggcc
      241 atgatcttag tggtgcgatc ttttgcgacc tttttgcagg aacaggtatt gtaggccgtg
      301 cttttaaaaa agccgtgaat aaagttataa gcaatgatct ggagtattat agttttgtcc
      361 tgaaccagaa ctatataggg aatatccagg agatcccaaa ccaggaggaa ctgattgacc
      421 ggttgaacag tgtggcgctg cgcaaagggt ttattcattc ccactatagt ctgggcggat
      481 caagtcgcca gtatttttca gaaactaacg ctcaaaagat cgatgctgtg cgccttaaga
      541 ttgaagaatt aaagctgagc caaaacattg ataactgcgc gtattatttt ctgctggcta
      601 gtcttcttga gtcggctgat aaagtggcga ataccgcatc cgtctatggt gcctttctta
      661 agcgtttgaa aaaatcggcc caaaaagaac tgatcctgaa aggagcccat tttgatttaa
      721 gtttaaacgc aaacgaagta tatcagcagg acgcgagcga actgatagga aagatctctg
      781 gcgatatcct gtatctggat ccgccgtata atgcaagaca gtatggtgcc aactaccatt
      841 tgttaaatac catcgctgct tatacaccct ttacccctaa gggtaagacg ggactgccat
      901 cataccaaaa aagttcattt tgcagtcgct ctcaaatctt aaatgctttt gaaaatctga
      961 ttaagaaggc gcgttttaaa tacatttttc tgtcttataa caacgaagga ctgatgagcg
     1021 aaatagagat caagaatatt ctgaagaaat acggtgccta ttcattgatg actaaaacct
     1081 acatgcgttt caaagccgac aacaagcggg ctcataaagc ggcacatact aaagaatgtc
     1141 tgcacatctt gatcaaataa
//
