LOCUS       New Construct      1247 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1247
                     /gene="M.HpaII-CCGG#1"
                     /codon_start=1
                     /translation="MKDVLDDNLLEEPAAQYSLFEPESNPNLREKFTFIDLFAGIGGF
                     RIAMQNLGGKCIFSSEWDEQAQKTYEANFGDLPYGDITLEETKAFIPEKFDILCAGFP
                     CQAFSIAGKRGGFEDTRGTLFFDVAEIIRRHQPKAFFLENVKGLKNHDKGRTLKTILN
                     VLREDLGYFVPEPAIVNAKNFGVPQNRERIYIVGFHKSTGVNSFSYPEPLDKIVTFAD
                     IREEKTVPTKYYLSTQYIDTLRKHKERHESKGNGFGYEIIPDDGIANAIVVGGMGRER
                     NLVIDHRITDFTPTTNIKGEVNREGIRKMTPREWARLQGFPDSYVIPVSDASAYKQFG
                     NSVAVPAIQATGKKILEKLGNLYD*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaaagacg
      181 tgcttgacga taacttgttg gaggaaccag cagcacagta ttcactgttt gaacccgaga
      241 gtaatccgaa tcttagagaa aaatttactt tcatcgacct gtttgccggt attggtggct
      301 tcagaatcgc aatgcaaaat ttaggtggta aatgtatatt ctcatctgag tgggatgaac
      361 aggcccaaaa aacgtatgag gcaaattttg gggacctgcc ttatggagat attacattgg
      421 aggaaactaa agcatttatc ccggagaagt ttgacatttt gtgcgcgggc tttccgtgcc
      481 aggcattctc gattgcagga aagcgcgggg ggttcgaaga tacccgggga acactgtttt
      541 tcgatgttgc agagattatc cgtcgtcatc aacccaaagc atttttttta gaaaatgtaa
      601 agggacttaa gaatcatgat aaaggtcgta cgctgaagac aattcttaat gttttgcggg
      661 aggacttagg ttattttgtg ccggagcctg cgatcgttaa cgctaagaac tttggcgttc
      721 ctcaaaaccg tgaacggatt tatatcgtgg ggtttcataa gtcaacaggg gtgaatagtt
      781 tcagttatcc tgaaccgctg gataaaattg ttacctttgc cgacatacgt gaggagaaaa
      841 cggtcccgac gaaatattat ttatccacgc aatacataga cacgttgcgt aaacataaag
      901 aacgtcacga atcgaaaggt aatggttttg gttatgaaat catcccggat gatggaatcg
      961 ccaacgcaat cgtggttgga gggatgggac gggaacgcaa cctggtaatt gaccaccgca
     1021 ttacggattt cacccctacg actaacatca aaggcgaagt caatcgtgag ggcatccgca
     1081 aaatgacgcc ccgtgaatgg gcgcggttgc agggcttccc tgactcatac gttattccag
     1141 tatcggatgc aagtgcctat aaacagttcg gcaactctgt ggccgtcccg gctattcagg
     1201 cgaccgggaa aaagattctg gaaaaactgg gcaatttgta tgattaa
//
