LOCUS       New Construct      1427 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1427
                     /gene="M.MspI-CCGG#2"
                     /codon_start=1
                     /translation="MKPEILKLIRSKLDLTQKQASEIIEVSDKTWQQWESGKTEMHPA
                     YYSFLQEKLKDKINFEELSAQKTLQKKIFDKYNQNQITKNAEELAEITHIEERKDAYS
                     SDFKFIDLFSGIGGIRQSFEVNGGKCVFSSEIDPFAKFTYYTNFGVVPFGDITKVEAT
                     TIPQHDILCAGFPCQPFSHIGKREGFEHPTQGTMFHEIVRIIETKKTPVLFLENVPGL
                     INHDDGNTLKVIIETLEDMGYKVHHTVLDASHFGIPQKRKRFYLVAFLNQNIHFEFPK
                     PPMISKDIGEVLESDVTGYSISEHLQKSYLFKKDDGKPSLIDKNTTGAVKTLVSTYHK
                     IQRLTGTFVKDGETGIRLLTTNECKAIMGFPKDFVIPVSRTQMYRQMGNSVVVPVVTK
                     IAEQISLALKTVNQQSPQENFELELV*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaaaccag
      181 aaatcctgaa actgatccgc agcaaactgg acttgacgca gaagcaagcg agtgaaatta
      241 ttgaagtgtc tgataagaca tggcagcaat gggagtcagg caaaactgaa atgcacccgg
      301 catattacag ttttcttcag gagaaactga aggacaaaat aaacttcgaa gaactgtcgg
      361 cgcaaaagac tttacagaaa aaaattttcg acaaatacaa tcagaatcag atcaccaaga
      421 acgcagagga gcttgccgaa ataactcaca tcgaggaacg caaagacgca tatagttccg
      481 atttcaagtt tattgatctg tttagtggta ttggcgggat tcgtcagtca tttgaggtaa
      541 atgggggcaa gtgtgttttc agttctgaaa tcgatccgtt tgcaaaattc acatactata
      601 cgaattttgg cgtggttccg tttggcgata ttacgaaagt cgaagccacc accattccgc
      661 aacatgatat tctgtgtgcc ggttttccat gtcaaccatt ttctcatatt ggaaaacgtg
      721 aagggttcga gcatcctaca cagggtacta tgttccatga gatcgtaaga attatcgaaa
      781 caaaaaaaac cccagtgctg ttcttagaaa acgtgccggg gctgataaat catgatgatg
      841 ggaacacttt aaaagtaatc attgaaacac ttgaagacat gggttataag gtacatcata
      901 ccgtattaga tgcctctcac tttggcattc cgcaaaagcg caagcgcttt tatttggttg
      961 catttctgaa ccagaatatt cactttgagt ttcccaaacc acccatgatc agcaaagaca
     1021 tcggagaggt cttggagagt gatgttactg gatattcaat ctcagagcac ctgcagaaat
     1081 catatctttt taaaaaagat gatggtaagc catcgttaat cgataaaaat acaacaggcg
     1141 cggttaaaac tctggtgtca acctaccata agatacaaag attaacagga acctttgtca
     1201 aagacggtga aacgggtata cgtttgctga caactaacga atgtaaagcc ataatggggt
     1261 ttcctaagga ctttgtaata cctgtctcgc ggacccagat gtatagacag atgggcaact
     1321 cggtggtcgt cccggttgtg actaaaatcg ctgaacagat atcgctggct ctgaaaaccg
     1381 tgaatcagca gagccctcag gaaaacttcg aactggaatt agtgtaa
//
