LOCUS       New Construct      3601 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..2189
                     /gene="Cbei_3161.1"
                     /codon_start=1
                     /translation="MDNDLNFVSFLWNIAESLRGTYKEEDYRKVMIPMIVVRRFDCLL
                     DDYDKEIIKKVYSNYDYMPEEEIDEIVIADLKENHNIDLQFYNVSDFTWKKLLDDSEN
                     IKANFEEYLNGFSNNVKEIIGKFNFKAEITQLDKKNKLYAVLQKMSEVDLHINKISNN
                     KMGYIYEEMLRRFTENSAAGEQYTPREVIKLCMEMLFLGKESFITEEGKVISIADFCC
                     GTGGMLSIAEAYVENLNEKAIVDVYGQELLDESFAICQADMLMKGQNPDNIRLGNTLT
                     EDRFSGEHMRFLISNPPFGVTWKDEEKKVKDEADLGFDGRFGAGTPRVSDGSLLFLQN
                     MISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRRWIIENDMLEGIIALPTDMFYN
                     TGIATYIWVITNRKTENRKGKIQLVNATDFYVPMRKSLGNKRKEISTEQIQEIKSIYE
                     SFEPSENCRIFDNKEFGYRKITIERPLKLSFKVDEEAISKVKETTQFINLAVSKKKDE
                     AAKASEEALGKEVQNKIIEMLQSFDSNEVYLNREEFIKKVKSKAKNYDLTLGAALLKA
                     IWSSIGERNEDADICKDSKGNPESDSSLKDTESIQLKEDINAYFEREVKPHVPDAYMD
                     ETTFSNIGYEIPFTRHFYKYEKLRAFSDIMKEVEDLEQEIAVEIKKVLG"
     misc_feature    2190..2191
                     /product="Cbei_3161.2"
                     /label="Cbei_3161.2"
     CDS             2192..3601
                     /gene="Cbei3160-S"
                     /codon_start=1
                     /translation="MKFRYRSEEEMKDSGVKWIGKIPRDWEVSKIKYIKSPDKNSFVD
                     GPFGSNLKSEHFIENGEVYVIESNFATQGILKLDSLKKISTEHFETIKRSEVKENDIV
                     IAKIGAQFGLSNILPRIDKKAVVSGNSLKLSVDKQKSNTQYIHYQLLHIKNNGTLDLI
                     VSTTAQPAISLGDMNNINIVLPNVQRQDKIVKFLNEKTAQVDSIISKKEALIQILEEA
                     KKSLISDAVTGKVKVVKTSDGYELVERKKEEMKDSGVKWLGDVPKEWDVKRLRFLGNL
                     QNGISKSGDEFGFGYPFVSYGDVYKNISIPKFVNGLVNSSLNDRRIYSVLEGDVFFTR
                     TSETVDEIGFASTCLNTITDATFAGFLIRFRPFKDKLYKGFSKYYFRCDLNRKFFVKE
                     MNLVTRASLSQNLLNNLAVALPLYKEQQEIYSALEFKVGGIECSINKLRCQIQKLKEA
                     KQALISEAVTGKIKILDEI*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atggataacg
      181 acttaaactt tgtatctttc ttatggaata ttgcagagtc cttacgcgga acctataagg
      241 aggaagatta tcgtaaagtt atgataccta tgattgttgt gagacgtttt gattgtctgc
      301 tggacgatta cgataaagaa ataataaaaa aggtgtattc aaattacgac tatatgccgg
      361 aggaagagat cgatgaaatc gttatcgcgg atctgaaaga aaaccacaat atcgacctgc
      421 agttttataa cgtatcagat ttcacttgga aaaaattact ggacgactca gaaaatatca
      481 aagcgaattt cgaggaatat cttaacggat tcagcaataa cgtaaaagaa attatcggaa
      541 agttcaactt taaggctgag ataacgcaat tggataaaaa gaacaaatta tacgcggtgc
      601 tgcagaaaat gtcggaagtc gatctgcata tcaataaaat ttcgaacaac aagatggggt
      661 atatatatga agaaatgctg cgtagattta ccgaaaatag tgctgcggga gaacagtata
      721 ctccacgtga agtaattaaa ttgtgtatgg aaatgctttt tttgggtaaa gagtcattta
      781 tcactgaaga aggcaaagtc atatcgatag ctgatttttg ctgcggcacg ggtggtatgc
      841 tttctatcgc tgaagcatat gtggagaatt taaatgaaaa agcaattgtg gacgtctatg
      901 gccaggaact tcttgatgaa tcttttgcca tttgccaagc tgacatgctg atgaaaggcc
      961 aaaatccgga caacattcgt cttggaaata cattaaccga ggacagattt agtggagagc
     1021 atatgcggtt tttaataagt aaccccccgt tcggtgtcac gtggaaggat gaagagaaaa
     1081 aagttaaaga tgaagcagac cttgggttcg atggtagatt cggggccgga actccccgcg
     1141 tatcggatgg atcactgtta tttcttcaaa atatgatttc gaaaatgtat gacgatgaag
     1201 aaggatcccg cattgccatc atttttaacg gcagtccgct gttcacgggt gatgcgggta
     1261 gtggtgaatc taatatccgc cgttggatta tagaaaatga tatgcttgag ggtatcattg
     1321 ctctgccgac cgatatgttt tacaacaccg gaattgctac gtacatttgg gtcattacta
     1381 accgcaagac agaaaataga aaagggaaaa ttcaattggt taacgcgaca gacttctacg
     1441 tcccgatgcg taagagtttg ggtaacaaac gtaaggaaat atcgacagag cagatccagg
     1501 aaattaaatc catctacgag agttttgaac cgagcgaaaa ttgtcgtatt tttgataata
     1561 aagagtttgg ttatcgcaaa ataaccattg agagaccgct taaattaagc tttaaggtcg
     1621 acgaagaagc catttcaaaa gtgaaggaaa ctacacagtt cattaactta gccgtgtcca
     1681 aaaagaagga tgaagctgcc aaagcctcag aagaggcctt aggtaaagag gttcagaata
     1741 agatcattga gatgctgcag tcatttgatt ctaatgaagt ttatttaaac cgcgaagaat
     1801 ttattaaaaa agtcaaatcc aaggcaaaaa actatgattt aacgcttggt gcggcattgc
     1861 tgaaagccat ctggagttca atcggcgaac gcaatgaaga tgccgatatt tgcaaggact
     1921 ccaaaggaaa tcctgaaagt gactccagtc tgaaagatac cgaaagcatt cagctgaaag
     1981 aagacataaa tgcatatttc gagcgtgaag tgaaacctca tgtacctgat gcatatatgg
     2041 acgaaaccac cttttctaac attggctacg aaataccatt cacgcgccac ttttacaaat
     2101 acgaaaaact gcgcgccttt tcagatatca tgaaggaagt agaagatctg gaacaagaaa
     2161 ttgctgtcga gattaagaag gtgcttggat aatgaaattc cgctaccggt ctgaagaaga
     2221 aatgaaagat agtggcgtta aatggatcgg taaaattccc agagattggg aggtgagcaa
     2281 gattaagtat ataaaatccc ccgacaagaa ttcgttcgtc gacggccctt ttggcagtaa
     2341 tttaaagtcg gagcatttta tcgagaacgg ggaagtctat gttatcgaat ctaattttgc
     2401 cacacagggc atactgaagt tggacagtct gaaaaaaatt agcactgagc actttgaaac
     2461 aattaaacgc tctgaggtta aggaaaatga cattgtgatc gctaaaatag gtgcccagtt
     2521 cggccttagc aatattttgc cccgcatcga taaaaaagca gtggttagtg gaaacagcct
     2581 taaattgtca gttgacaagc agaaatcaaa tacacagtat atccactatc agttgctgca
     2641 tatcaaaaac aatggtaccc tggacttgat tgtatcaacc actgcacaac ctgcgatatc
     2701 gctgggtgat atgaacaata ttaacattgt tctgcccaat gtacaacgtc aggataaaat
     2761 tgtgaaattc cttaatgaaa agacggcaca agtcgattca ataatttcga agaaagaagc
     2821 attgattcag atacttgaag aggccaaaaa aagtttaata agtgacgcag taaccggaaa
     2881 ggtgaaagtt gtgaagacgt cagatgggta tgagttagtt gagcgtaaaa aggaagagat
     2941 gaaggactca ggggttaaat ggctgggtga tgttcccaag gagtgggatg ttaaacggtt
     3001 acgtttcctg ggcaatcttc aaaatggtat tagtaaaagt ggtgatgaat ttggtttcgg
     3061 ttaccccttt gtatcctatg gtgacgtata caaaaatatt tcgataccta aatttgtcaa
     3121 tggtcttgta aatagttcat taaacgacag acgtatatat tctgtactgg aaggcgatgt
     3181 cttctttacc cggacatccg agaccgtgga tgaaataggg ttcgcttcga cgtgtcttaa
     3241 tacaattacg gacgctacat ttgccggctt tctgatccgt ttccgccctt ttaaagataa
     3301 attatataaa ggcttttcga aatactattt tagatgcgat ctgaatcgca aattctttgt
     3361 gaaggaaatg aatctggtaa cacgtgcttc gttaagtcag aaccttttaa acaatttggc
     3421 cgtcgctctt cctctgtaca aggaacaaca ggaaatatat tctgcactgg agttcaaagt
     3481 tggcggaatc gaatgttcca ttaataagtt gcgttgccag atccagaaac tgaaagaagc
     3541 caaacaggcc cttattagtg aagctgtcac tggaaagatt aagatactgg acgagatcta
     3601 a
//
