LOCUS       New Construct      3806 bp    DNA         SYN       3-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..3806
                     /gene="CloclaDRAFT_1051"
                     /codon_start=1
                     /translation="MDKVVIKKFAVWARKKLIEDIKQKAYELGITEKEIKLPGFATSD
                     TAIIGDRSLSKKEIEQRKSLVSRIEEKGYNNVIEEVAYTWFNRFIALRFMEVNNYLPT
                     GVRILSSVEPGKKEPDIIKEALNIDLDLDRELVYKLQDENDTESLYRYLLIKQCNALN
                     KIFPGLFEKIEDYSEILLPSNLLAEGSVIRRLVEDISEEDFKEQVEVIGWMYQYYISE
                     KKDEVFEGLKKNKKITKENLPAATQLFTPDWIVKYMLENSLGRLWLEGHPDEELKSKW
                     KYYLEEAEQEPEVQKQLEEIWAKSKNIRPEDIKVLDPAVGSGHILVYAFDLLYDIYRN
                     AGYSERDIPKLILENNLYGLDIDDRAVQLAYFAVMMKARSKSRRIFKEKVKVNICAIQ
                     ESNGFPKEAIDYLVNTGETEIEKRLLREDVEYLINVFHDAKEYGSLLEVKPVDFDAIE
                     SRLEEIKKGEVQDLVEYQYRNIILEKIPPLVKQARIMSQKYDAVCTNPPYLGRRGMNS
                     KLAKFVEDSYTTGRNDLFAVFIKICLNYSKNYVSMITQHSWMFLSGFEELRKEILNLT
                     VIKNMLHLGTRTFEEIGGEVVQSVSFTISKIKVRGYNGLYIRLCDYMSSEEKRRNLFN
                     RNNYYTVNSDEFFKIEGAPIAYWASPKVKNIFSDSIKLGDIAYPRKGNSTSDNDRFLR
                     LWFEVDIDKVNFNAKKIIKEETIVRRWFPYNKGGGYRKWYGNNYYLIDWKNDAEEIRK
                     IPTAVIANYHYFMKPGLTWSTVSTGKFSIRIFGYGFIFDNGGCCLFTDEEDRLYYLAL
                     LNSNIFDYLLGLLNPTVNYQSGEIAKFPVVFAKSEDAGNRINSLAEDNIQIARIDWDS
                     FETSWDFKRHPLLAHKGDSITVQQAFNNWSAFAEKQFNRLKANEEELNRIFIEIYGLQ
                     DELSPEVEDRDITVRKAERVRDIKSFISYAVGCMFGRYSIDAEGLIYAGGEFKDKWKN
                     EDGKWKVRRIVKDDEGKVIEDLWVDAAFIPDMDNIIPVTDDEYFEDDIVKRFIEFLKV
                     TFGEETLEENLDYIADTVGKKADETSRQAIRRYFLREFYKDHVQVYQKRPIYWLFDSG
                     KENGFKALIYMHRYDEFTVARVRTDYLHKLQKSYEAEIKRLDIIIDSDASQREKAGAR
                     KKKEKILKQMEECRLYDQVIAHAANQRIEIDLDAGIKVNYARFQGIEIPRGNDRKALK
                     TDLLAKI*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atggacaaag
      181 tcgttattaa aaagttcgca gtgtgggccc ggaaaaaatt gatcgaagat atcaaacaga
      241 aagcctacga actggggatt accgaaaaag aaattaaact gcctgggttc gccactagcg
      301 ataccgctat tattggcgat cgctcccttt ctaagaagga aattgagcag cgtaaaagtc
      361 ttgtgtcacg catagaggag aaaggataca acaatgttat cgaagaggtg gcatacacct
      421 ggttcaatcg ttttattgct ctgcgcttta tggaggttaa caattattta ccgactggtg
      481 tacgcattct gtcttcagta gaaccaggaa aaaaggaacc cgacattatt aaagaagcgc
      541 tgaatattga cttggatctg gatcgcgaat tggtgtataa gcttcaagat gaaaatgaca
      601 ctgagagtct ttatcgttac ctgcttatca aacaatgtaa cgcgttgaat aagattttcc
      661 cgggactgtt tgagaaaata gaagactact cagaaattct tctgccgtca aacctgttag
      721 cagaaggtag cgtgatccgt cgcctggttg aagatatctc agaagaggat tttaaggagc
      781 aagtcgaagt tatcgggtgg atgtatcagt attatattag cgaaaaaaaa gacgaggttt
      841 ttgaaggcct gaaaaaaaat aaaaaaatca ccaaagagaa tcttccagcc gccactcagc
      901 ttttcacacc cgattggatc gttaaatata tgctggaaaa ctcgttaggt cgcctttggc
      961 tggaagggca tcctgatgaa gaactgaaaa gcaagtggaa atattattta gaagaggcag
     1021 agcaggaacc ggaggtgcag aagcagcttg aagaaatttg ggccaaaagt aaaaatatcc
     1081 gtcctgaaga tattaaggtc ctggaccctg ccgttggaag tggccacata ttggtatacg
     1141 cctttgattt actgtacgac atttatcgga atgcagggta ttctgaacgt gacattccga
     1201 aattaattct tgaaaataat ctttatggcc tggacatcga cgaccgtgcc gtacaactgg
     1261 catattttgc agtgatgatg aaagcgcgga gtaaatctag acgtatcttt aaggaaaaag
     1321 tgaaggtaaa catttgcgca atccaagaaa gcaatggttt cccaaaggag gctattgatt
     1381 acttagtgaa tacgggggaa acagaaatcg aaaaacggtt attgcgtgag gatgtagaat
     1441 acctgattaa cgtgttccac gatgccaaag agtatggcag tctgcttgag gtgaaaccgg
     1501 tagattttga cgccattgag agtcgtctgg aggaaataaa aaaaggagaa gtccaggatt
     1561 tagtggaata tcagtacaga aacataatcc ttgaaaaaat tccacctctg gtgaagcagg
     1621 cgagaatcat gtcacagaaa tatgatgcag tatgcactaa tccgccatat cttggaagac
     1681 ggggcatgaa cagcaaactg gcaaaatttg tcgaagactc atatactacc ggtcgcaatg
     1741 atttatttgc cgttttcatt aaaatttgcc ttaattattc caaaaattat gtcagtatga
     1801 tcacgcagca ttcatggatg ttcctgtcgg gctttgagga acttcgcaaa gaaatcttaa
     1861 acctgacggt tataaaaaac atgttacacc tgggaacccg caccttcgag gaaattggag
     1921 gtgaagtcgt tcaatcggtt agctttacca tttccaaaat taaagtacgt ggatataacg
     1981 gtctttatat acgtttgtgc gactatatgt ccagtgagga gaagcgccgc aacctgttta
     2041 atcggaacaa ttattacaca gtgaattcag atgagttttt taaaattgaa ggtgctccaa
     2101 ttgcgtattg ggcgtcaccg aaagtaaaga atatttttag tgattctata aagctggggg
     2161 atatagcata tcctcgtaag ggcaattcta catcggacaa tgatagattt ttgcgtctgt
     2221 ggtttgaagt tgacatagat aaggtgaact ttaacgcaaa aaagatcatc aaagaggaga
     2281 ctattgtccg ccgctggttc ccgtataaca agggtggtgg gtaccgcaaa tggtatggca
     2341 acaactacta tctgattgac tggaagaatg acgccgaaga gatacgcaaa atcccgaccg
     2401 cagtaattgc caactaccac tattttatga aaccgggtct gacctggtcc acggtctcaa
     2461 cgggcaaatt ctcgatccgt atttttggtt atggctttat tttcgataac gggggttgct
     2521 gtctgtttac cgatgaagaa gatcgcctgt actaccttgc gctgcttaac agcaatatat
     2581 ttgattattt gcttggcttg ctgaatccta ctgtcaatta ccagtcgggc gagattgcca
     2641 agtttccggt agtgttcgca aagagcgaag atgcggggaa tcggatcaat agtcttgcgg
     2701 aagataatat tcagattgct agaatcgatt gggattcgtt tgagacgtct tgggatttta
     2761 aacggcaccc cttactggcg cacaaagggg attcgatcac tgtgcaacag gcgttcaata
     2821 actggagtgc ctttgctgaa aaacagttca atcgtctgaa agccaatgaa gaagaactga
     2881 atcgcatttt cattgaaata tatggcttac aggatgaatt gtccccggaa gttgaagata
     2941 gagatattac ggtacgtaaa gctgagcgtg taagagatat taagtctttt atcagctatg
     3001 cagtaggttg tatgttcggg cgctacagca tcgacgcgga aggtcttatt tatgctggcg
     3061 gtgagttcaa agataaatgg aagaatgaag acggtaaatg gaaagtgcgt cggatcgtta
     3121 aagacgatga aggtaaagtt attgaggact tatgggtcga tgcagcgttt attccagaca
     3181 tggataacat tatcccggtg accgacgatg aatatttcga agatgatatc gtgaaacgct
     3241 ttatcgaatt tctgaaagtg acttttgggg aagaaactct ggaagaaaac ttagattata
     3301 ttgcggatac cgtagggaag aaagcggacg agaccagccg ccaggccatc cgccggtatt
     3361 ttttacggga attctataaa gatcatgtgc aagtttatca aaaacgccca atctattggc
     3421 tttttgattc aggaaaagaa aatgggttta aagctctgat atatatgcac cgttatgatg
     3481 aatttactgt cgcccgggtt cgcacagact atttgcataa acttcagaag tcatatgaag
     3541 ccgaaattaa acgcttagac ataatcattg atagcgacgc gagccagcgt gagaaagcag
     3601 gcgctcggaa gaaaaaggag aaaatcttaa aacagatgga agagtgtcgt ctgtatgatc
     3661 aagttatagc tcatgcagca aatcagcgta ttgagatcga cctggatgcc ggtatcaaag
     3721 tgaattatgc gagatttcag ggcattgaga ttccgcgtgg caacgatcgg aaagctctga
     3781 aaacagattt gctggctaaa atttaa
//
