LOCUS       New Construct      3833 bp    DNA         SYN       3-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..3833
                     /gene="CloclaDRAFT_1058"
                     /codon_start=1
                     /translation="MDKAAIKKFAVWARKKLIEDIKQKAYELGITEKEIKKPDVSTSD
                     TVIIGDRSLNRKEIEQRNSLVSRIEEKGFNNVIEEVAYTWFNRFIALRFMEVNGYLPT
                     GIRVLSSIEHDRKEPDIIREALNIDLDLERELVYKLQDDNDTESLYRYLLVKQCNALN
                     EILPGLFEKIDDYTEILLPSNLLAEGSVIRHLVEDISEDDFREQVEIIGWMYQYYISE
                     KKDEVFEGLKKNIKITKENIPAATQLFTPDWIVKYMVENSLGRLWLEGHPDEELKSKW
                     KYYLDEAEQEPEVQKQLEEIRAKSKDIRPEDIKVLDPAMGSGHILVYAFDVLYDIYKS
                     AGYSERDIPKLILENNLYGLDIDDRAAQLAYFAVMMKARSKSRRIFREKINVNVCSIQ
                     ESNGFPKEAMDYLVNPEETEIEKRLHREDVEYLINVFQDAKEYGSILEVKPIDFDAIE
                     RRLEEIRDGEIQDLIEYQFKNIIIERIPPLIKQAKIMSQKYNVVCTNPPYMGDDGINL
                     KLSKFLKENYKDTRSDLFAVFIEKTIKFSTKSSYISMITQNSWMFLRSYEKLRGMILN
                     GATINSMVHLGPRAFEEIDGEVVRTTAFVLNNNVLNGYKGIYIRLVDIYKSLDKEKEY
                     FVLKNRYNGVKQNSFKRIPGSPIAYWCSEMVANSFIDGIILKNISEPKQGLITGNSSA
                     FLRIWYEVNIENIGFKMKDREEAIVSKKKWFPINKGGEYRKWYGNNEYIVNWQNDGIE
                     IKNYKDEKGKLKSRPQNIEYYFKKGLTWTKVTSAKFSVRFTEDGFIFSEAGMKIFVHD
                     NLLDYIGSFLNSNLVNVLLGALSETINYEQGNVARLPLKICNDININEKISHLFKTNV
                     DISRVDWDSFENSWDFRKHPLLNYKFNGRTIEQAFNNWSAFAEEQFNQLKTNEEELNR
                     IFIQIYGLQDELTPEVEDKDITIRKADRERDIKSFISYAVGCMFGRYSIDAEGLIYAG
                     GDFKDKWKNEDGQWKVRKTVKDDESKIIEDTWVNATFVPDMDNIIPITDDEYFEDDIV
                     SRFVEFLKVTFGEETLEENLDYIADTIGRRTTETARQTIRRYFLKEFYKDHVQVYQKR
                     PIYWLFDSGKEDGFKALIYMHRYDELAVARVRTDYLHKLQKSYEAEVKRLDIIIDSNA
                     SQREKANARKKREKILKQMEECLQYDQVIAHVANQRIKIDLDDGVKVNYAKFQGIEVP
                     QGEGRKPLKADLLAKI*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atggacaaag
      181 cagcgataaa aaaatttgcc gtctgggcgc gtaaaaaact gatcgaagac atcaaacaga
      241 aagcgtatga acttggtata acagagaaag aaattaaaaa accagatgtc tctacctccg
      301 acacggtcat cattggtgat cgtagcttga accgtaaaga aattgaacag cgtaactcgt
      361 tagtgagccg tattgaagaa aaagggttca acaacgtgat agaggaagtg gcctatactt
      421 ggtttaatcg ctttattgct ctgcgtttca tggaagtaaa cggttatctg cctactggga
      481 ttcgtgtcct gtcatcaatt gaacacgatc gcaaagagcc agacataatt agagaagcgc
      541 tgaacatcga cctggacctg gaacgtgaat tggtttataa acttcaggat gataatgata
      601 ccgagtccct gtatcgctat ctgttagtaa aacaatgcaa cgccttgaac gaaattttac
      661 ccggtctgtt tgaaaaaatc gatgattata cagaaatctt attgccttcc aatctgttgg
      721 cggaaggttc cgttattcgt caccttgtcg aagatattag tgaagacgat ttccgcgagc
      781 aagttgaaat cattggctgg atgtatcagt attatatatc agaaaaaaag gatgaagttt
      841 tcgagggcct gaagaaaaac atcaaaatta caaaagaaaa tataccagcc gcaacgcagc
      901 tgtttactcc ggactggatt gttaaataca tggttgaaaa tagcttgggg cgtctgtggc
      961 tggaaggtca tccagacgaa gagttaaaat cgaaatggaa gtactacttg gatgaagctg
     1021 aacaggaacc tgaggtccaa aagcaactgg aggagatacg tgcgaagtcc aaagatattc
     1081 gcccggaaga cataaaagtt ctggatccag ctatgggttc cggccatatt ttggtttacg
     1141 cgtttgatgt cctgtatgat atttacaaat ctgccggcta ttctgaacgt gatataccga
     1201 aactgatttt agaaaataac ctgtacggct tagatattga cgatcgtgct gcgcagttgg
     1261 catactttgc cgttatgatg aaagccagat ctaaatcacg ccgcattttt cgtgaaaaaa
     1321 taaatgttaa tgtctgttcc attcaggaat ccaatggctt tccgaaagag gcaatggact
     1381 atctggttaa cccagaagaa accgagattg aaaaacgtct gcatcgcgaa gatgttgagt
     1441 atctgattaa cgtctttcag gatgccaagg aatatggtag tatcttggaa gttaagccaa
     1501 ttgattttga cgccattgaa cgccgtttgg aagagatccg cgacggtgag atccaagatt
     1561 tgatagaata ccaattcaaa aacattatca ttgaacgcat acccccattg atcaaacagg
     1621 ccaaaattat gagccaaaag tacaatgtag tttgtacaaa tccaccttat atgggagacg
     1681 atggcataaa ccttaagttg tcaaagtttc tgaaggagaa ctataaagat actcggtcag
     1741 atttatttgc cgtgtttata gaaaagacga taaaattttc aacaaagagt tcatatatca
     1801 gtatgattac acaaaatagt tggatgtttc tgcggagcta cgaaaaactg cggggtatga
     1861 tcttgaatgg tgccacgatt aattccatgg tacatcttgg tccgagagct ttcgaagaga
     1921 ttgatggaga agtggttcgt acaactgcat ttgttctgaa caacaacgta cttaacggtt
     1981 ataaaggtat atatatccgg cttgttgata tatataagag cctggataaa gagaaggaat
     2041 atttcgtatt aaagaatcgg tacaacggtg ttaaacagaa tagcttcaaa cggattcctg
     2101 gaagtccaat tgcctattgg tgcagcgaaa tggtcgctaa cagcttcata gatggcataa
     2161 tactgaagaa cataagcgag cccaaacagg gcctgattac aggcaattca agcgcgtttc
     2221 tgcggatttg gtatgaagtg aacattgaaa atattggatt caaaatgaag gaccgcgaag
     2281 aagccatcgt tagtaaaaaa aaatggtttc cgatcaataa aggcggggaa tatcgcaaat
     2341 ggtacggcaa caacgaatat atcgtgaatt ggcagaacga tggaattgag atcaaaaatt
     2401 ataaagacga aaaaggtaaa ctgaaatctc gcccacagaa tatagaatac tattttaaaa
     2461 aaggcttgac ctggacgaaa gtcactagcg cgaaattttc agttagattt acggaggatg
     2521 gcttcatctt ttcggaagcg ggaatgaaaa tttttgtaca tgataatctg ctggactata
     2581 ttgggtcatt tctgaactcg aatctggtta atgtcttact gggtgcactt agcgagacaa
     2641 tcaactacga acaaggaaac gtggccagat tgccactgaa gatatgcaat gatataaata
     2701 ttaacgagaa gatatcccac ctgtttaaaa ccaatgtgga tattagccgc gtggattggg
     2761 attcttttga aaacagttgg gattttcgta aacatcctct gctgaattat aagttcaacg
     2821 gcagaacgat agaacaggca tttaacaact ggtctgcatt tgctgaagag caattcaatc
     2881 aattaaaaac gaatgaggag gaacttaatc gcatttttat tcagatctat ggcctgcagg
     2941 acgagctgac ccccgaagtg gaggataaag acatcacgat tcgcaaagca gatcgtgagc
     3001 gtgatataaa aagctttatt agctatgcag tcggctgcat gtttggtcgc tattcgatag
     3061 atgccgaggg tcttatatat gcaggtggtg atttcaaaga taaatggaaa aatgaggatg
     3121 gtcagtggaa agttcgtaag actgtcaaag acgacgaatc aaaaataatt gaagatacct
     3181 gggtgaacgc tacatttgta cctgatatgg acaacattat acctataaca gatgacgagt
     3241 atttcgaaga cgatatcgtt tcacgcttcg ttgaattctt aaaggtgacg tttggggaag
     3301 aaaccctgga agagaattta gattacatag ccgacaccat aggccgcaga acaacggaaa
     3361 cggcgcgtca gacgatacgg cgctacttcc tgaaagaatt ttataaagac catgtacaag
     3421 tctaccagaa acgccctata tattggctgt ttgatagtgg gaaagaagat ggttttaaag
     3481 cgcttattta tatgcatcgc tatgatgaac tggctgtggc gcgtgtacgc acagattatt
     3541 tgcacaaact gcagaagagc tatgaggccg aagtgaaacg cctggacatc attatagatt
     3601 ctaacgcttc gcagcgtgaa aaggcaaatg ctcggaaaaa acgcgaaaaa attcttaaac
     3661 agatggaaga atgcttgcag tatgaccagg tgatagcaca cgttgcgaac caacgcatta
     3721 aaattgatct tgacgatggt gttaaagtta attacgcgaa gtttcaaggc atagaggttc
     3781 cgcaggggga gggtcgtaaa cctctgaagg cggatcttct ggccaaaatc taa
//
