LOCUS       New Construct      2946 bp    DNA         SYN       3-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1337
                     /gene="CloclaDRAFT_1830S"
                     /codon_start=1
                     /translation="MTEWREQKLSDFMDFNPYTPLSKGIIAKKVTMEKLIPFNRKIQG
                     YEDAVFSGGTKFKNGDTLVARITPCLENGKTAYVDFLNDEEVAFGSTEFIVLRAKEGI
                     SDSRFIFYFAISDEFRDTAIQLMSGTSGRQRVDTEALKRKVFTLPPLPEQKAIAEVLS
                     SLDDKIDLLTKQNKTLEDLAQAYFRKWFIEDASKEWEVVPISEKFDVLLGGTPSRKIE
                     SYWTNGTIGWINSGKINEFRIIEASEYITEEALNNSSAKLLPAGTTVLAITGATLGKI
                     SMVLRSFAANQSVIGLVPKAELSNNFIFLWLKENINALISMQTGGAQQHINSNDVKSF
                     DVIVPDTVALSLFRRKIDPLMLKISQNCFQINTLNKLRDTLLPKLISGEIRVKM*"
     misc_feature    1338..1353
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1354..2946
                     /gene="CloclaDRAFT_1831M"
                     /codon_start=1
                     /translation="MAKVKKSKNEVKEQPIEQVLWAAADKLRKNMDAAEYKHVVLGLV
                     FLKYISDSFYDLYYKLKEGKGEYEGADPDDPYEYRAENVFYVPPQARWDYLQSRAKLP
                     TIGKDIDEAMEAVEKDNPSLKGVLPKEYAKEKLDKQSLGGLIDLIGTIALGDSVSKSS
                     DILGQVYEYFLGQFALAEGKKGGQFYTPRSVVQLLVEMLEPYEGRVFDPCCGSGGMFV
                     QSEKFVEAHRDHYNGKSRGIDKLFERVVSIYGQESNQTTWRLCKMNLAIRGIDSTNVL
                     WNSEGSFLNDAHPDLKADFVIANPPFNDSDWSGELLRNDGRWKYGVPPVSNANYAWIQ
                     HFLYHLSPKGTAGFVMAKGSLSSKTNGEGEIRKNIIEAPLVDCIVNLPTKLFLNTQIP
                     ACLWFLSREKETNSNHPRRNKILFIDARNMGTLINRRTRELTDDDIRKIADTYHSWKK
                     DVGSYEDIPGFCKSATLDEVRELDYVLTPGRYVGLPEEDDDFDFEERVRKLTAELKEY
                     MEESAKLDERIKENLAKVGIEL*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgacggagt
      181 ggcgggaaca gaagcttagc gatttcatgg atttcaatcc atatactcca ctgtcaaaag
      241 gcatcattgc taaaaaagtt acgatggaaa agctgattcc attcaaccgg aaaatacaag
      301 gttatgaaga tgcggttttt agcggtggca cgaaatttaa aaatggggac actctggtag
      361 cgcgtatcac gccctgttta gaaaacggaa agactgcata tgtggatttt ctgaacgatg
      421 aagaagtagc ctttggcagc actgaattta ttgttttacg ggcaaaagaa ggcatttcgg
      481 atagccgttt cattttttac ttcgctattt cagacgaatt tcgcgatacc gcaatccaat
      541 tgatgagtgg cactagtggg cgccagcgtg tcgacacaga ggctttgaaa agaaaggtat
      601 ttaccttacc gccgcttccg gaacaaaagg ctatcgcgga agtattgtct agtttagatg
      661 acaagatcga cttgctgacc aagcaaaata agacgttaga agatttggca caggcctatt
      721 ttcgtaaatg gtttatcgag gatgcgtcta aagaatggga agtcgtccct atctcggaaa
      781 aatttgatgt cttattaggg ggaaccccgt cacgcaagat tgaatcatat tggaccaatg
      841 gaacgattgg atggatcaac tcaggcaaaa ttaacgaatt ccgcattatt gaggccagcg
      901 aatatattac ggaagaagct ctgaataata gtagcgcaaa actgctgccg gctggcacga
      961 cagtgttggc cattactggc gcgaccctgg ggaaaatttc aatggtgctg cggtcatttg
     1021 cagcgaatca atcggtcata ggactggtac ccaaggcaga attgtctaat aattttattt
     1081 tcttatggct taaagaaaat attaatgccc tgatttctat gcagaccggt ggagcacagc
     1141 aacatataaa tagtaatgac gtgaagtcct ttgacgtgat tgttcctgat actgtcgccc
     1201 tgagtctgtt ccgtcggaaa atcgacccgc tgatgttaaa aatttctcaa aattgttttc
     1261 agataaatac actgaataag cttcgcgata ctttattacc aaaattgata agtggcgaga
     1321 ttcgcgttaa aatgtaaata ggaggtgttc aatatggcca aagtaaaaaa atcaaagaat
     1381 gaggtcaagg agcagcccat agaacaagtg ttatgggcag ccgcagacaa actgcgtaaa
     1441 aacatggatg cagcggaata taaacatgta gttttgggtc tggtgtttct gaaatacata
     1501 agtgattcgt tttacgacct ttactacaaa ttgaaagaag gaaaaggtga atatgaaggg
     1561 gcagatccgg acgaccccta cgaatatcgt gctgaaaatg tgttttatgt tcccccgcag
     1621 gcccggtggg attacctgca gagccgcgcc aaattaccta ccattggaaa agatatagac
     1681 gaagctatgg aagcggtaga aaaagataat ccctctctta aaggagtact gccgaaagag
     1741 tacgcgaaag agaaattaga taaacagtcg ctgggcggct taattgatct gattggaaca
     1801 attgcactgg gtgactcggt ctctaaatcg tcagatattc ttggacaggt ctatgaatac
     1861 tttttgggtc agtttgcgct ggctgaaggg aagaaggggg gtcagtttta cacgccgaga
     1921 tctgttgtgc agctgctggt cgagatgctg gaaccgtatg agggtcgtgt ctttgatccg
     1981 tgctgcggtt ctggaggtat gtttgtgcag tcggaaaagt ttgtggaagc tcatagagat
     2041 cattataatg gaaaaagccg cggcattgat aaattgtttg agagagtcgt cagtatttat
     2101 ggccaggaaa gcaaccagac cacctggcgc ctgtgtaaaa tgaatttggc tattcgtgga
     2161 attgattcta ctaatgtcct ttggaacagc gaaggtagtt tcctgaatga tgcgcatccg
     2221 gatttgaaag ccgattttgt tatagcaaac ccccctttca acgattcaga ttggtcgggc
     2281 gaattattac gcaatgacgg tcgttggaaa tatggggttc cacccgtatc aaacgcaaac
     2341 tacgcctgga ttcagcattt cctttatcat ttgagcccaa aaggcacagc gggtttcgtg
     2401 atggctaaag gttccctgtc tagtaaaacc aatggcgaag gagagatccg caaaaatatt
     2461 atagaagccc cactggtaga ctgcattgtg aatttgccaa ctaaactgtt cttaaatacg
     2521 cagatcccgg cctgtctgtg gttcctgagc cgtgaaaaag agacaaatag taatcatcct
     2581 cgtcgtaaca aaattttatt cattgacgcc cggaacatgg gtaccctgat taaccgtcgc
     2641 acccgtgaac ttacggacga cgacattcgg aaaattgctg atacctacca ttcatggaaa
     2701 aaagacgttg gatcttacga agacattccg ggtttctgca aatcggcaac cctggatgag
     2761 gttcgcgagc tggactatgt tttaacgcct gggcgctatg tcggcctgcc cgaagaggat
     2821 gatgatttcg attttgagga gagagttcgg aagcttactg cggaactgaa agagtatatg
     2881 gaagagtctg ccaagctgga cgagcgtatc aaagaaaatt tagctaaagt gggtattgaa
     2941 ctgtaa
//
