LOCUS       New Construct      2778 bp    DNA         SYN       3-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1508
                     /gene="CloclaDRAFT_1869"
                     /codon_start=1
                     /translation="MNIQKISVEKLNPAAYNPRKDLKPGDKEYEKLKRSIEEFGYVEP
                     VIWNQKTGNVVGGHQRLKVLLDLGQTEIDCVVVDLDPQREKALNLALNKIQGEWDENK
                     LAELMAELDAGAFDVSLTGFDASEIDELLNRWYSKEAVQDSFDIDKAHEEIVQREPVT
                     KRGDIWLLGNHRLMCGDSTKDEDFEKLMEGCHAQMAVTSPPYGVGKEYEKAGIEPWFE
                     TVRPVIRNLCRYADIVCWNLGDLYATGSQFIEPTSVYSVNMFLENGYRPIWIRIWKKQ
                     GQNFGVGPYHLVSNKPVQQYEYISAFSNKGEVEEYNDQEYVWLSAFAGHSYKFVKRLT
                     KEERKKWGYAGIWEMTTVRANKEHPAMFPVELPWRCIKMHSDKGGIVLEPFSGSGTTI
                     IAAEQTERRCYAMELSPVYCDLAVKRWEDFTGEKAVKLLPHEAGFMGAPGLED*"
     misc_feature    1509..1524
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1525..2778
                     /gene="CloclaDRAFT_1868"
                     /codon_start=1
                     /translation="MEIQKIPAEKLKAAKYNPRKDLKPGDPEYEKLRRSIEEFGYVEP
                     VIWNRRTGNIVGGHQRYKVLTALGYKEIDCVVVDLDEQREKALNVALNKISGEFDIPL
                     LTDLLKGLNEDGFDVSLTGFDAAEIDELFRDKTAANVKEDNFDAEKAVSEIKMPVTQR
                     GDIWLLGRHRLMCGDSALLSDVQKLMAGQKARFVFTDPPWNVDYGSDARHPSWKPRQI
                     LNDRMSTEEFGAFLLSAFNCMREVSEPGCMTYVVMSAQEWGNVMNALREAGYHWSSTI
                     IWKKDSLVLSRKDYHTQYEPIWYGWLEGTRLCPLKDRKQSDVWEIPRPKVSEEHPTMK
                     PVSLVAKAMLNSSHTGDLALDLFGGSGTTMIAAEQTGRVCFMMELDPKYCDVIAKRYV
                     SQFGDNAAFLLRGDEKIPYAETQIA*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaatatac
      181 agaaaatctc agtcgaaaaa ttgaaccccg ctgcctataa tcccagaaaa gatttgaagc
      241 ctggagataa ggaatacgaa aagttgaaac gctcgatcga ggagttcggc tatgttgagc
      301 ctgtgatttg gaaccagaaa acaggtaacg tggtaggggg ccatcagcgg ctgaaggttt
      361 tactggactt ggggcagaca gagattgatt gtgtagtggt tgatctggat cctcaaagag
      421 agaaagcact gaatctggcc ctgaataaaa ttcagggaga atgggacgaa aacaaactgg
      481 ctgagttgat ggcggagctg gatgcaggcg cgtttgatgt atcgctgaca ggcttcgacg
      541 caagcgaaat tgatgaatta ctgaatcgct ggtattccaa agaagccgtt caggactctt
      601 ttgacatcga caaggcacat gaagagattg tacaaagaga gccagttaca aaacgtgggg
      661 atatttggct gctgggaaac catagactta tgtgcggcga ctcgaccaaa gacgaagact
      721 ttgaaaagct gatggagggg tgtcatgcac agatggctgt cactagccca ccgtatggcg
      781 ttggtaagga gtatgagaag gccggtatcg aaccgtggtt tgaaacagtt cgtcccgtta
      841 ttcgcaattt atgtcggtac gccgacatcg tgtgttggaa tctgggtgat ttgtacgcga
      901 ctggttccca gtttattgaa cctacatctg tatacagtgt taatatgttt ttagagaacg
      961 gttatagacc aatttggatc cgtatttgga agaaacaggg tcagaacttc ggagtaggtc
     1021 cctatcattt ggtctcgaat aaaccagtgc agcagtacga atatatttca gccttttcca
     1081 acaaaggaga agttgaagag tacaacgatc aagaatacgt ttggcttagt gcctttgcgg
     1141 gtcacagcta caaatttgtt aagcgtctga ctaaggaaga acgcaaaaaa tggggttacg
     1201 cagggatttg ggaaatgacg actgtacggg cgaataaaga gcatcctgcc atgtttccgg
     1261 ttgaactgcc ttggcgctgc attaagatgc actcagataa aggcgggatc gtgctggaac
     1321 cattcagcgg ctccggtacc accataatag cagcggaaca gaccgagcgc cgctgttacg
     1381 caatggagct gtcacctgtc tattgcgatt tagcggtaaa acgctgggaa gatttcacag
     1441 gcgagaaagc ggtgaaactt ctgccacatg aagccggatt tatgggcgct ccaggtttag
     1501 aggactgaat aggaggtgtt caatatggaa attcaaaaaa tcccggcaga aaaattgaaa
     1561 gcagcaaaat ataatccacg taaagatctt aaacccggag atcctgaata tgaaaagtta
     1621 cgtcgctcaa ttgaagagtt cggttatgtg gaaccggtca tctggaatcg ccgtactggt
     1681 aatattgtgg gagggcatca gcgttacaaa gtattgaccg cgcttggtta taaagaaatt
     1741 gattgcgtgg tagtagacct ggacgagcag cgtgagaaag ctttgaatgt ggcgcttaac
     1801 aagattagtg gtgagttcga tatcccttta ctgaccgatt tactgaaagg gctgaatgag
     1861 gacggctttg atgtatcttt gacaggtttc gatgccgctg aaatcgatga gttgtttcgc
     1921 gataaaacag cagcaaatgt aaaagaagat aattttgatg cagaaaaagc agtgtcagaa
     1981 attaaaatgc cggtgacgca gcgtggagat atttggttat taggtcgtca tcgcctgatg
     2041 tgtggggatt ctgcgctgct gagcgacgtg cagaaactga tggcggggca aaaggcgcgt
     2101 ttcgtattta cggatcctcc ctggaatgtt gattatgggt ctgatgcccg tcaccctagt
     2161 tggaaaccga gacagattct gaacgaccgt atgtccacag aagagtttgg tgcatttttg
     2221 ctttccgctt tcaattgcat gagagaggtg agtgagccgg ggtgcatgac ttacgtcgta
     2281 atgtccgcac aagaatgggg aaatgttatg aacgctctgc gggaagcagg gtatcattgg
     2341 tcctctacga ttatttggaa aaaagacagc ttggtactta gtcgtaaaga ctaccatacc
     2401 caatacgagc ctatctggta tggatggctg gagggcacgc ggctgtgccc actgaaggat
     2461 cgcaaacaaa gcgacgtctg ggaaatacca cgtcccaagg tgtctgagga acaccccaca
     2521 atgaaaccag tctcattagt tgctaaagcc atgttaaata gctctcacac aggagacctt
     2581 gccctggatt tatttggagg ctcaggaact acgatgattg cagctgaaca aaccggacgg
     2641 gtatgcttta tgatggaatt agatcctaaa tactgcgatg tgattgcaaa acggtacgtt
     2701 tcacaatttg gtgataatgc cgcattctta ttgcgcggtg atgagaaaat cccgtacgcg
     2761 gaaacacaaa ttgcataa
//
