LOCUS       New Construct      2940 bp    DNA         SYN       3-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1484
                     /gene="CloclaDRAFT_2368"
                     /codon_start=1
                     /translation="MTKWNNVSLGELLTESNIPSENPDPSKRITVRLNVEGIEKRPFR
                     EETKGATKYYIRKAGQFIYGKQNLHKGAFGIIPEELDGYESTIDLPAFDIDKSKVLPE
                     WLMLILTKDNFYKELESIASGSATKRIHPERLFQVKIPLPSISEQKDILKKYDMYKVY
                     HTAISNIIDDARIYIDKLRQSILQEAVQGKLVPQDPNDEPASVLLERIKEEKERLIKE
                     GKIKKEKPLPPISEDEIPYELPEGWEWVRLGEIAIINPRNDIDDDVEVSFIPMKLIED
                     GFSNKHTSEIKKWKDVKNGFTHFQEGDVVIAKITPCFQNRKSAVMRNLKNGYGAGTTE
                     LYVVRTYANTVLPEYLLALFKTEAFIKGGVATYTGTAGQQRVKKDYIENLLLPLPPLN
                     EQKRIVEKVDQLMALCDELEKNIEQSKKDCELLIQSVLQEAFKEA*"
     misc_feature    1485..1500
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1501..2940
                     /gene="CloclaDRAFT_2369"
                     /codon_start=1
                     /translation="MSISATIKAVQDIMRQDAGVDGDAQRISQLVWMIFLKVFDAKEE
                     EWELMDDDYTPIIPEGLRWRDWAADDEGITGDELLDFVNNKLFKELKEMKLDENSNPK
                     AFIVKAVFEDSYNYMKSGTLMRQVINKLNEIDFTTQKDRHLFNDIYESILRDLQSAGN
                     AGEYYTPRPVTQFMVDMVNPQLGEKVLDFACGTGGFLVCALEHLKKQVKNIEDEKTLQ
                     ETILGIEKKPLPYMLAVTNLILHDIDVPKIRHDNSLARNVRDYKPVDKVDIIVTNPPF
                     GGIEEDGILVNFPQQFQTKETADLFMVLLMYLLKDTGRAAIVLPDGFLFGEGVKTTIK
                     EKLLEEFNLHTIVRLPNGVFAPYTDINTNLLFLEKGKPTKEIWFFEHPLPEGYKKYTK
                     TKPIRHEEFELEKQWWNNREEKEYAWKVSIEEIKNRNYNLDIKNPNNNRSNELESTEK
                     IIEKLDYNFKKLSLILNEIKDSLFREGVN*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgacaaaat
      181 ggaataacgt aagcctgggt gaattgttaa cggagagtaa tatccccagc gagaacccgg
      241 atccgagtaa aagaatcaca gttcgtttaa atgttgaagg catcgaaaaa agaccgtttc
      301 gtgaagaaac gaaaggtgct actaaatatt atattcgcaa agccggtcag tttatttatg
      361 ggaaacagaa cctgcacaag ggggcttttg gaatcattcc agaggagctg gatggttacg
      421 agtctaccat agacctgcca gcttttgata tcgataaatc aaaggtactg cccgagtggt
      481 taatgttaat tctgaccaaa gataattttt ataaagagct ggagagcatt gcatcaggga
      541 gtgcaacaaa gcgcattcat ccagaacgtc tgttccaggt caaaatcccg ctgcctagca
      601 tttctgaaca aaaggatatt ttaaagaaat atgatatgta taaagtatat cataccgcaa
      661 tcagtaacat cattgatgac gcccgtattt atattgacaa actgcgtcag tcgatcttgc
      721 aggaagcggt ccaagggaaa ttagtacctc aggatccaaa cgatgaacca gcgagcgtgt
      781 tattggaacg tattaaggaa gaaaaagaac ggttaattaa ggaaggtaaa attaaaaaag
      841 agaaaccgct gcccccgatt agcgaagatg agataccgta tgagttacct gagggttggg
      901 agtgggttcg tctgggagaa attgcaatca taaaccctcg taacgacatt gatgatgacg
      961 tggaagtttc ctttatcccg atgaaattaa ttgaagatgg attttcgaat aaacacacga
     1021 gtgaaattaa gaaatggaaa gacgtgaaga atggctttac tcattttcag gaaggtgatg
     1081 tagtgattgc aaaaatcacg ccatgtttcc agaaccgtaa atccgcggtg atgcgcaatc
     1141 tgaaaaacgg ttatggggca ggtacgactg aattgtatgt cgttcgcacc tatgccaata
     1201 cggtcctgcc agaatactta ctggcattgt ttaagaccga agccttcatt aaagggggcg
     1261 tagccaccta caccggcacc gcgggtcagc agagagtgaa gaaagattat attgagaacc
     1321 tgttgttgcc actgccgccg cttaatgaac agaaaagaat tgtggagaag gtggatcaac
     1381 tgatggccct gtgtgatgaa cttgaaaaaa atatcgagca atccaaaaaa gactgcgagc
     1441 ttctgataca gagcgtgctg caggaagcat tcaaagaagc ctaaatagga ggtgttcaat
     1501 atgtcgattt ccgccaccat taaagcagta caggatataa tgagacagga tgctggtgtt
     1561 gatggggatg cgcaaagaat atctcaatta gtttggatga ttttcctgaa ggtgttcgac
     1621 gcgaaagaag aagagtggga acttatggat gatgattaca ccccgattat ccctgaaggt
     1681 ctgagatggc gtgattgggc cgccgacgat gaaggcatta cgggggatga attactggat
     1741 ttcgtcaaca acaagttatt caaagagctg aaagagatga aattagatga gaacagcaac
     1801 ccgaaagcat ttattgtcaa ggctgtgttt gaggattcat ataactatat gaaatctggt
     1861 acgttaatgc ggcaggttat caataagctg aatgaaattg atttcacgac gcagaaagat
     1921 agacacctgt ttaatgatat atatgaaagt atcctgcggg atctgcaatc tgcgggtaat
     1981 gctggggaat attatactcc gcgtcctgtt acacaattta tggtggatat ggtcaacccc
     2041 caattgggcg agaaggtatt agactttgca tgtggcactg ggggcttcct ggtctgtgca
     2101 ctggaacact taaaaaaaca agttaagaat atagaagatg aaaaaactct gcaggaaaca
     2161 atattgggca tagaaaaaaa accattgccg tacatgcttg cagttacgaa cctgattctg
     2221 cacgatatag atgtgcccaa gatccgtcat gataatagcc tggctagaaa cgtgcgtgat
     2281 tataaacctg ttgataaggt tgatattata gtaacgaacc ctcccttcgg agggattgaa
     2341 gaagacggca tcttggtgaa ctttccgcag cagttccaga caaaggagac ggcggacttg
     2401 tttatggtgc tgctgatgta cttacttaag gatacaggga gagcagcaat agtactgccg
     2461 gacggctttt tgtttggtga gggtgtgaaa acgacaatta aagaaaaact gttggaggaa
     2521 tttaacttac ataccatagt acgtctgcct aatggtgttt tcgcgcctta cacggatatt
     2581 aacacgaatt tactttttct tgaaaagggc aagccgacaa aagagatatg gtttttcgaa
     2641 catccgctgc cggaaggtta caaaaaatac acaaaaacga agccgattcg ccatgaagag
     2701 ttcgaactgg agaaacaatg gtggaataat agagaagaaa aggaatacgc ctggaaagtt
     2761 agcattgaag aaattaaaaa tcggaattat aaccttgata ttaaaaatcc gaataataat
     2821 cgttcaaacg aattggaatc caccgagaag attattgaga aactggacta taatttcaaa
     2881 aaactgtccc ttattcttaa cgaaattaaa gatagtttat tccgcgaagg tgtcaattaa
//
