LOCUS       New Construct      2726 bp    DNA         SYN       7-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..2726
                     /gene="Clo1313_2367"
                     /codon_start=1
                     /translation="MKKLTKEDIDKVRHIEGFPIANDEDIIALSNPPYYTACPNPFIE
                     DFIKEHGKPYDEATDDYHREPFAADVSEGKNDPIYNAHSYHTKVPHKAIMRYILHYTE
                     PGDIVFDGFCGTGMTGVAAQMCGNPDPELKARIEAEMPYVKWGARKAILNDLSPAATF
                     IAYNYNTPVDVAEFEKEAKRILEECEKEYGWMYETQHTINGEIQYGIDGKPIMGRINY
                     TVWSDVFICPTCSNEIVFWDAAVDKEKGKVNDTFYCPHCNSQLTKRSCERAQETHFDA
                     RLNEFVTMAKQVPVLINYSVGKKRFEKTPDEYDLALIEKIDNMDIPYWYPTDRMCEGS
                     ESRRNDRYGITHVHQFYTKRNLYLNAWLRNKIIKVKDKCISDILLFGFNNVQQRHCKL
                     NAMRFNVSFPSNITSGTLYLPSMIRENNIIDQLSNKYFKRIVKAFKDNTSKYSIVSTN
                     STTSNIINSNNSIDYIFTDLPFGSNLNYSELNFLWEAWLKVFTNQEPEAIINAVQGKG
                     LLEYQSLMTRCFEECYRVLKPGRWMTVVFHNSQNSVWNAIQESLMRAGFVIADVRTLD
                     KQQGSFKQVTTTSAVKQDLVISAYKPKESFKREFISHAGSEETAWSFIQQHLEKLPVV
                     VVKNGKIELIEERKAYLLYDRMVAYHIMNGIPVPLDATDFYKGLDERFLKRDGMYFLP
                     DQVNEYDTARIVNDVEPIQMELLVTNEKSAIAWLYKQLETPQTYAELQPKFMQEIRAW
                     DKFEKRPELSELLEENFLQNEEGKWYIPDVTKASDVAKLREKKLLKEFEGYLATKGKL
                     KLFRTEAIRVGFAKLWADKNYKLIVETAERLPESVILEDDKLLMYYDLSLGRI*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaaaaaat
      181 taactaagga agatattgat aaagtccgtc atatcgaagg tttcccgatt gcaaacgatg
      241 aagacattat tgcgctgagt aatccccctt actataccgc ctgtccaaat ccctttatcg
      301 aagatttcat aaaggagcac ggcaaaccct atgatgaagc gaccgatgac taccatagag
      361 aacctttcgc agctgacgtt tcagagggga aaaatgaccc gatttataac gcgcatagtt
      421 atcatactaa agtgccgcac aaagcaatta tgcgttacat cctgcattat actgaacctg
      481 gcgacattgt ttttgatgga ttttgcggaa ccggtatgac cggagtagcc gctcagatgt
      541 gcggtaatcc tgacccagaa ttgaaggcgc ggatagaagc agaaatgccc tacgtaaagt
      601 ggggtgcgcg taaagcaatt ttaaacgatc tgtctcccgc tgctacattc atcgcgtata
      661 actataacac gccggtcgac gtggcagaat ttgaaaaaga agcgaaacgt atactggagg
      721 aatgcgagaa agaatatgga tggatgtatg agacacagca tacgatcaac ggcgagattc
      781 aatatggcat agatggaaaa cccattatgg gccgcataaa ttatactgtt tggtcggatg
      841 ttttcatttg ccctacttgc agcaacgaga ttgtgttctg ggatgcagcg gttgataaag
      901 aaaaaggcaa agtcaacgat acattttatt gtccgcattg caatagtcaa cttacaaaac
      961 ggagttgcga acgggcacaa gaaacccatt ttgatgctcg tctgaatgaa tttgttacaa
     1021 tggccaaaca ggtgcctgtc ttaatcaatt atagcgtggg aaaaaaacgt tttgagaaaa
     1081 ctccggatga atacgatttg gctctgatcg aaaaaattga caacatggat attccttatt
     1141 ggtatccgac ggatcgtatg tgtgaaggca gcgagtcacg ccgcaacgac cgttatggta
     1201 ttactcacgt tcatcaattt tacacgaaac gcaatctgta tctgaatgca tggttacgta
     1261 ataaaataat taaagtaaaa gacaagtgta ttagtgacat tctgcttttt ggcttcaaca
     1321 acgtccagca gcggcactgt aaattgaacg caatgcgctt taatgtgtca ttccccagta
     1381 atatcacaag cgggacgctg tacttaccgt ctatgatcag agaaaataat atcatcgacc
     1441 agttatctaa taaatatttc aaacgcattg ttaaggcatt caaagacaat acttctaaat
     1501 attccattgt ttccactaac agtaccacat ccaatatcat aaattcgaat aacagtattg
     1561 actatatatt caccgatctt ccgttcggtt ctaatctgaa ctatagcgag ttgaattttc
     1621 tttgggaggc ttggctgaaa gttttcacaa accaagaacc ggaagcgata ataaacgctg
     1681 ttcaggggaa aggtttactg gaataccaga gtttgatgac acgttgtttc gaagaatgtt
     1741 accgcgtctt aaaaccaggc cgctggatga cagttgtgtt tcataattcg cagaattctg
     1801 tgtggaatgc tattcaggag agtctgatgc gtgcaggctt tgtcatcgcg gatgtccgta
     1861 cattggacaa acagcagggg agttttaagc aggttacgac gaccagcgcc gtaaaacaag
     1921 acctggttat aagcgcatac aagccaaaag agtctttcaa aagagaattc attagtcatg
     1981 cgggtagcga agagactgcc tggtctttca tccagcagca cctggaaaaa cttccagtgg
     2041 ttgtggtgaa aaatggtaaa attgaactga tagaggaacg gaaggcgtac ctgttatacg
     2101 atcggatggt tgcctaccat attatgaatg ggattccagt tccactggat gcaacggatt
     2161 tttataaggg ccttgacgag cggttcctta aacgtgatgg catgtacttc ctgccggacc
     2221 aagttaacga atatgatacg gcacgtatcg taaatgacgt cgaaccgatt caaatggaac
     2281 tgcttgtcac taacgaaaaa tcggccattg cctggctgta taaacagttg gaaactccgc
     2341 agacatatgc agaattacag ccgaaattca tgcaagaaat cagagcgtgg gataaatttg
     2401 aaaaacgtcc cgaattaagt gaactgctgg aagaaaactt cttgcagaat gaggaaggca
     2461 agtggtacat acccgatgtg actaaagcgt cagatgtggc gaaattgcgg gagaaaaaat
     2521 tgttaaagga gttcgagggc tatttagcga ccaagggaaa gttaaagctg tttcgcacgg
     2581 aggcgattcg cgttggtttt gccaagctgt gggcagacaa gaactataag cttattgttg
     2641 aaaccgcaga gcggctgcct gaaagtgtta tattagagga tgataaactg cttatgtatt
     2701 atgacttatc acttggccgt atttaa
//
