LOCUS       New Construct      4005 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..2615
                     /gene="Cob47_0799"
                     /codon_start=1
                     /translation="MASDKITLRQLETHLFRAADILRGKMDASEYKEYIFGMLFLKYT
                     SDVFEEKRQELRDRFQNMRFSEEQIQELLEDPVSYSDAFFVPEKARWENILKLKEDVG
                     NQLNKALSALEEANPELDGVLKHIDFNAVKGKTRLKDQQLIDLINHFNKYKLTPSNFE
                     FPDLLGAAYEYLLKEFADSAGKKGGEFYTPSHVKKLMVRLVKPREGMSIYDPTVGSGG
                     FLIEAFHYVEEQGQNPRNLALYGQELNGLTWSICKMNMILHGINDAHIENEDVLTTPM
                     FSENGYIKRFDRILANPPFSENYSRANMQFEERFKYGFTPENGKKADLMFLQHMIASL
                     KDDGVMATVMPHGVLFRGGQEKVIREGIVRDDLIEAIIGLPPKLFYNTGIPACIIVIN
                     KNKPEHLKNKILFINADREYGEGRNQNFLRPEDIEKIVTVFDEKKEIPKYSSLVDIKE
                     IEENDFNLNIRRYVDNSPDPEIEDVRAHLFGGVPKSEVLLYEKQLRKFNLSYDILLAE
                     KSEDYLEFKKDITDRNQIRELIDNCTEVKITIGKHKEKLLEWWQVVRTQIEQFYGKNN
                     LWKFRNEALEKLKDSLLPIGTFDEFKIAGIFANWWEELVYDFKSIVSADWNKNLVERE
                     RIKEKYFKKDIEEIEDLESRLAEIEADLSELLEEVEDWDEEEQGDKTASKVKEFLKDL
                     VKNLKSKDSEAARREARKWEDLLENIVQKEKEIKNVKSEIGRKQKELENKIDEKIESI
                     TKEEAKELLIEKFFELISNQLEKYLNAEKKELIKIFEKLWDKYHVSLEHLLKEREQEV
                     SRLNKFLEELGYYERV*"
     misc_feature    2616..2631
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             2632..4005
                     /gene="Cob47_0801"
                     /codon_start=1
                     /translation="MGHNGVIKGSDGGNVKVANNAEFPKEWTIVSLERDCVLISGLRP
                     KGGASDEGIPSLGGEHITLDGRINFSDVNAKYIPEKFFKIMTKGKAEENDILINKDGA
                     NTGKVAILKKKFYKDIAINEHLFILRSKKLFVQQYLFYWLFSRFGQKQITDRITGSAQ
                     PGLSSTFIKNFLVPRPPLSEQRKIAEILETIDNAIEKTDAIIEKYKRIKQGLMQDLLT
                     KGIDENWQIRNEKTHKFKDSLLGRIPEEWKVVKLKDVADIRLSNVDKKTDLKGKIIQL
                     CNYLEVYQNDYIIKGMNFMHASATNNEIKKFKISKGDVIITKDSEEYNDIAKPAYVRD
                     EIENLICGYHLALIKPLNNINGLFLSKVLSFRNVNIYFQQRANGITRFGLTKETITGA
                     IIPLPLIPEQERIATILSQIDEVIEKEQAYKEKLERIKKGLMEDLLTGKVRVNHLLIE
                     EEGKDDN*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atggctagcg
      181 acaaaataac attgcggcaa cttgaaaccc atttattccg tgcagcagat atacttagag
      241 gcaagatgga tgcctctgag tataaagagt atattttcgg catgctgttt ttaaaatata
      301 cttctgatgt ctttgaagaa aaacgccagg aattacgtga ccgtttccaa aacatgagat
      361 tctcggaaga gcagattcag gagctgttag aagacccggt gtcttactct gatgccttct
      421 tcgttcctga gaaagctcgc tgggagaaca tcctgaaatt aaaggaagac gtaggcaatc
      481 agctgaataa agcactgtct gcccttgaag aggcaaatcc ggaactggac ggtgtcttaa
      541 agcatattga cttcaatgca gtgaaaggta aaactcgtct gaaagatcag cagttgatcg
      601 atctgattaa tcactttaac aaatataaac tgacgcctag taatttcgaa ttccctgact
      661 tattaggagc agcgtatgaa tatttgctta aagagttcgc ggattccgca ggcaagaaag
      721 gaggcgagtt ttataccccc tctcacgtga agaagctgat ggttcgtctg gtgaaaccgc
      781 gcgagggcat gtcaatatac gatccaaccg tggggtctgg tggttttctg attgaggcgt
      841 ttcattatgt cgaggaacaa ggccaaaacc cacgcaactt agctctttac ggacaggagc
      901 tgaatggcct gacatggtca atctgcaaaa tgaatatgat cctgcatgga atcaatgacg
      961 cacacattga aaatgaggac gtcttgacca cccccatgtt ttcggagaat ggctatatta
     1021 agcgttttga tcgcattctt gccaatccac cgttctccga aaattattcc cgcgcgaata
     1081 tgcagtttga agagcgtttc aaatatggtt ttacccccga aaacggtaaa aaagccgatc
     1141 ttatgttttt acaacatatg attgcgagcc ttaaggatga tggtgtgatg gcgacagtga
     1201 tgccgcatgg ggtcctgttc cgtgggggtc aggaaaaagt gatccgtgag ggtatcgttc
     1261 gggatgactt aatagaagct attatcggtt tgccgcccaa attattttat aacaccggga
     1321 tcccagcatg catcattgtg attaacaaaa acaagcccga acacttaaag aataaaattc
     1381 tgttcataaa tgccgataga gagtacggag aaggtcggaa tcagaatttt cttcgcccag
     1441 aagatatcga aaagatagtg accgtatttg atgaaaaaaa ggaaataccg aaatattcta
     1501 gtcttgtcga tatcaaggag atagaggaga acgattttaa cttaaatatt cgcagatatg
     1561 tagacaactc accggatcct gagatagaag acgttcgcgc acacctgttt ggcggcgtac
     1621 cgaaatccga ggtcttactt tatgaaaaac agttacgtaa atttaacctg tcctatgata
     1681 tattactggc cgagaaatca gaagattatt tagaattcaa aaaagacata accgacagaa
     1741 accagattcg tgaactgatc gataactgta cggaggtgaa aattactata ggaaagcata
     1801 aagaaaaact tctggaatgg tggcaggtgg ttcgcaccca gatcgaacag ttttatggca
     1861 agaacaatct gtggaaattc cggaacgagg ccttggaaaa gctgaaagac tcattgcttc
     1921 ccattgggac atttgacgag ttcaaaatag cgggaatttt cgcaaactgg tgggaagaat
     1981 tagtgtatga cttcaaatct attgtgagcg cagattggaa caaaaatctt gttgaacggg
     2041 aacgtattaa agagaaatac ttcaaaaaag atatagaaga gatcgaagac ctggagtccc
     2101 gcctggctga aattgaagct gacctgtcag agctgctgga ggaagttgaa gattgggacg
     2161 aagaggagca gggtgataaa acagctagta aagtgaagga attcctgaaa gatctggtaa
     2221 aaaatctgaa gagtaaagat tcagaggcag ctcgccgcga agcgagaaag tgggaagatc
     2281 tgttagaaaa tattgtgcag aaagaaaaag aaattaaaaa tgtaaaaagt gaaattggtc
     2341 gcaagcagaa ggaacttgaa aacaaaatag acgagaaaat cgaatccatc acaaaggagg
     2401 aagctaagga attactgatc gaaaagtttt tcgaactgat tagtaatcag ttggaaaaat
     2461 atctgaacgc agaaaaaaaa gaactgatta agatttttga aaagctttgg gataaatacc
     2521 acgttagtct tgaacatctg ctgaaagagc gtgaacaaga ggtttcccgt ttaaataagt
     2581 tcttggaaga actgggatat tatgaacgcg tctaaatagg aggtgttcaa tatgggccac
     2641 aacggggtga taaaaggttc ggacggtggt aatgttaagg tggcaaacaa tgccgaattt
     2701 ccgaaggagt ggacaattgt gtctctggaa cgcgattgcg tcctgatttc tggacttaga
     2761 cctaaaggcg gcgcctcgga tgagggcatc ccgagcttag ggggcgaaca tataacattg
     2821 gatggacgta taaacttttc agatgtcaat gctaaatata ttccggaaaa attttttaaa
     2881 attatgacga agggaaaagc agaggaaaat gacattctta tcaacaaaga cggtgcaaac
     2941 accggaaaag tggcaatact gaaaaagaag ttttacaaag acattgccat taatgaacat
     3001 ctgtttattc tgagaagcaa aaaacttttt gtgcaacagt acctttttta ctggctgttc
     3061 tcgcgttttg gtcagaagca gataaccgat cgtattacgg ggagcgctca gccaggtctt
     3121 tcctctacat ttattaagaa tttccttgtt ccgcgccccc cgctgtcaga gcagcgtaag
     3181 attgcggaaa tcttggaaac tattgataac gccatagaga agactgatgc aattattgaa
     3241 aaatataaac gtataaagca gggcctgatg caggacttgc tgaccaaagg aatagatgaa
     3301 aattggcaga ttcggaatga aaaaacccat aaattcaaag attctctgct gggtcgtatt
     3361 cccgaggaat ggaaggtggt caaactgaag gatgttgccg atataagatt atccaacgtt
     3421 gataaaaaga cagatttaaa aggtaagata atccaactgt gcaactatct ggaggtgtat
     3481 cagaatgatt atatcatcaa aggtatgaat tttatgcatg cttctgcgac gaacaatgaa
     3541 attaagaaat ttaagatcag caaaggtgat gtgataataa cgaaagattc tgaggaatat
     3601 aatgacattg caaaacccgc ttatgtccgg gacgagatcg aaaacttgat ttgcgggtat
     3661 catctggctt tgattaaacc gttaaacaat atcaacggac tgttcctgtc taaggtcctg
     3721 tcgttcagaa atgtgaacat atactttcaa cagcgcgcca atgggattac tcggttcggg
     3781 cttactaagg aaaccattac gggggccatc attccgctgc cgctgattcc ggaacaagag
     3841 cgcattgcaa caattttgag ccagatagat gaagttattg agaaagaaca agcgtacaaa
     3901 gaaaagcttg agcgcataaa gaaaggtctt atggaggatc ttttgactgg caaggtacgg
     3961 gttaatcact tgttgattga agaagaaggt aaggacgata attaa
//
