LOCUS       New Construct      3490 bp    DNA         SYN       7-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..2057
                     /gene="M.CthIV"
                     /codon_start=1
                     /translation="MQELKDKIKKLGYEEIKDIDDATFIASHKNVYVYVKKVDEEQLK
                     PELVTAITYEAMATDPISTYAWITNGTSNAYVLVEEEKAVSEIPSVFEDENKLYSGKR
                     QLTDRDKWSIRKYQELQEKFDGLHEMIYGMKDHVNNSNDVIDEFSKLIFLETFRLYHP
                     EYRLTKGNVTGKLFNEIYRYEYVEKHKDKAVQEIREAFKEIKDHADYVAILDNGEKAN
                     IFSADEYIKLENPNIYIAVLKALQDLGTIIIDGVERPATLRDLTGDVLGRVFDVLLRG
                     KFENKGGMGIYLTPRQVTEAAAEMVLHDLTKDGAAKLIAKDPKTGIPTLRIGDLCCGS
                     GGFLIKMLQKIEHYLLNKLTGDKKQYEELFEQMKEHCFIGADNAPGMVLKARINMALH
                     GAPKCPIFQTRNSLMNTRLKPGTFDAILTNPPFSKTGISKTIKKGKTTVENPEGAEII
                     KYYSSDIDEDGQNRMSPYGLSLGSKPDSRGKWKEVNSVDPAVLFIDRNLQLLKPGGLL
                     MIVVPDGILSNSGDKYVREYIMGKKNPVTGEFEGGKAILKAVISLPQVTFALSGAGAK
                     TSLLYLKKKEHPGEKQGPVFMAVADEVGFTVKQNVEVQLGDDHNDLLKIVEAYKKGMP
                     EDVE*"
     misc_feature    2058..2068
                     /product="Ctherm spacer"
                     /label="Ctherm spacer"
     CDS             2069..3490
                     /gene="S.CthIV"
                     /codon_start=1
                     /translation="MINNVLVWKQPNISWIHPINIDNSINANNFNFDYLDTLNKLRSS
                     NLKILELRDIADKISDGPFGSQLKVEEYKEQGFPVYRVKNIIDTQILDDDIVYIDAKK
                     QQQLKRSEVLPGDVLITKAGRIGSAAVVPSKFGNGNITSHLVLVRLKKTINNYYLVAY
                     LECKYGKVITGRESYKSTRPELTKNEIGNVIIPIPSPEIQKYIGDKVRKAEELREEAK
                     RLKKEAETFLYEMIQLKPLNDFDKDMFSFVNSNYIDSERLDSEYYKTKYITLEKLLKS
                     KKVTSFKDIIIESKYGASVPADYTMVGIPFIRGNNLTDNEINIDDIVYLNKKLKDEVK
                     DHHVNTGDILITRSGTVGISAVVDEKCDGFSFGSFMIKLRIDMRIWNPYYIAAFLNSF
                     WGKWQIERLQNGAVQQNINLQEIGRIIIPIISKENQDKIEELIKNYINKKRQSKQLIQ
                     EAKQDVEDLIEGNFDMSKVKANS*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgcaggagc
      181 tgaaagataa aatcaaaaaa ttagggtacg aggagatcaa ggatattgat gatgccacat
      241 ttattgcctc tcataagaat gtctatgttt atgtgaaaaa ggttgatgag gaacagctga
      301 aaccggaact tgtgacagcg attacatatg aggccatggc aacagatcct atttctacct
      361 acgcatggat aacaaacggc acgagtaatg cctatgtgct tgttgaagaa gaaaaagctg
      421 tgtcggaaat ccctagtgtg tttgaagacg aaaataaact gtactctggt aaacgccaac
      481 tgactgaccg tgataagtgg agcatccgta aatatcagga acttcaggaa aaatttgacg
      541 ggctgcatga aatgatttat ggcatgaagg accatgtcaa caatagtaac gatgtgatcg
      601 atgagttttc taagctgata ttcctggaaa cattccggct gtaccatccg gagtaccggt
      661 taacgaaagg gaatgtaacc ggcaaattat tcaacgaaat ctatcgctat gagtatgtag
      721 aaaaacataa ggataaggct gttcaagaga ttcgtgaagc gttcaaagaa attaaggacc
      781 atgcagatta tgtggcaatc ttagataacg gtgaaaaagc taatattttt agtgcggatg
      841 aatatatcaa gctggagaac ccgaatattt acatcgcagt gttaaaagca ctgcaggatt
      901 tgggcaccat cattatagat ggagtagaga gacctgcgac tcttcgggat ttaacaggcg
      961 atgtattggg acgtgtgttc gatgtactgc tgcggggcaa attcgaaaac aagggaggaa
     1021 tgggcattta cttgacacct agacaagtta ccgaagcagc agcggaaatg gtcctgcatg
     1081 atctgacaaa ggacggggcg gccaaattaa ttgccaaaga tcctaaaact ggtattccaa
     1141 ctttgcgcat cggcgatctg tgttgtggct ctggtggttt tcttataaaa atgctgcaaa
     1201 aaattgaaca ttatcttctg aataaactta caggagataa aaaacagtac gaggaattat
     1261 tcgaacagat gaaagaacat tgttttatcg gtgccgataa cgcacctggg atggttctta
     1321 aagcccgtat caatatggca cttcatggag ccccgaaatg cccgattttc cagacacgca
     1381 atagtctgat gaatacacgc cttaaacctg gcacttttga tgcgatcctt accaatcccc
     1441 cattttcaaa gaccggtatt agcaaaacga ttaaaaaagg taaaactacg gtggagaacc
     1501 ctgaaggcgc agaaatcatc aaatattatt cgagtgacat agatgaggat ggccagaatc
     1561 ggatgagtcc ctatggttta tcgttagggt cgaaacccga ctcaagaggt aagtggaaag
     1621 aagttaacag tgtagatccg gctgtcctgt ttattgatcg taatttgcaa cttcttaaac
     1681 caggcgggtt gcttatgata gtagttccgg atggaatcct gagcaatagt ggggataaat
     1741 atgtgcggga atatattatg ggtaagaaaa atccagttac cggtgagttt gagggtggca
     1801 aagcgattct gaaggccgtg ataagtctgc ctcaagtgac tttcgctctg agtggagccg
     1861 gggcgaagac ctcgctgctg tatctgaaaa aaaaggaaca ccctggagaa aaacaaggcc
     1921 cggtttttat ggcagtcgca gatgaggtcg ggtttactgt gaaacagaac gtcgaggttc
     1981 agttaggtga cgatcataat gatcttctga aaatagtaga agcttataaa aaaggcatgc
     2041 cggaagatgt cgaataggtg gtgtagcaat gataaacaac gtgctggtgt ggaaacaacc
     2101 caatatatct tggatacatc ctattaacat cgataattcc atcaatgcaa ataattttaa
     2161 ctttgattat ctggatactc ttaataaact gcggagctcc aaccttaaaa tcttagagct
     2221 gcgggatatc gctgacaaaa ttagtgatgg cccatttggt agccaactta aagtcgagga
     2281 atataaagaa caagggtttc cagtttatcg cgtgaaaaat ataattgaca cacagatttt
     2341 ggatgatgat atcgtctata ttgacgccaa aaaacagcaa cagttaaaac gttcggaggt
     2401 gctgccaggt gatgtattga taacgaaggc aggcagaatt ggttcagctg cggttgttcc
     2461 aagcaaattt ggtaacggca atatcacatc acacttggtt ctggtacggc tgaaaaaaac
     2521 tattaacaat tattatctgg tcgcttacct ggaatgcaag tatggaaaag ttattacggg
     2581 acgtgagagt tataagagta cccgccctga actgactaag aatgagatcg gtaatgtcat
     2641 tataccaata ccgtcaccag aaattcagaa gtatattggt gataaagtta gaaaagcgga
     2701 agaattacgt gaagaagcca agagattgaa aaaagaagcc gaaacgtttt tatacgaaat
     2761 gattcagtta aagccgctta atgatttcga taaggatatg tttagtttcg taaattcgaa
     2821 ttacattgat tctgaaagac tggattctga gtattacaaa acaaaataca ttactttaga
     2881 aaaattgctg aaaagcaaaa aagttacgtc ttttaaagat atcataattg aaagcaaata
     2941 cggcgcgtcg gttcccgccg attataccat ggttgggatt ccgttcatac gcgggaataa
     3001 tttaaccgac aatgagatca acatcgatga tatagtgtac ctgaataaaa agctgaagga
     3061 cgaagtcaag gatcatcatg ttaacacagg cgatattctg attacccgct cgggcaccgt
     3121 gggaatttct gcagtggttg atgagaaatg cgatggattt tcctttggaa gttttatgat
     3181 caaactgaga atcgatatgc gtatctggaa tccgtattac atcgccgctt tcttaaatag
     3241 cttttggggc aagtggcaga tagagagact tcagaacggg gccgtgcagc agaatataaa
     3301 ccttcaagag ataggccgta tcattattcc tattatctcg aaagaaaacc aggataaaat
     3361 agaagaactg attaaaaatt acataaataa gaagcgccag tctaaacaac tgattcagga
     3421 agccaagcag gatgtcgaag atctgataga aggaaatttt gatatgagta aggttaaagc
     3481 caactcttaa
//
