LOCUS       New Construct      1454 bp    DNA         SYN       7-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1454
                     /gene="Cthe_1148"
                     /codon_start=1
                     /translation="MNCVFITPDKMDGKRISYDYVVQQYEIQRLLQRRPLITLIDICK
                     EITSGIRVKKEYYTDKNGYKIIAPGDIRNEVIYINELKVVQPEVVREKDIINNGDILI
                     TASGKSGQVIYVNEVLEGCVVTSDIIKITLRDRDKGIRLYKFLKSSIGQMLLNSIKIG
                     ILNKIFVEDVENLLIPEDFDTYQEDCSDDSTVYAEAEKLYRSAENIFYRVFDYKGEKK
                     NLKHFYVTEYLDSHRLDPEYYSNFYTELYRVIHKNFDDVKWEELGELVEIKKADKPEI
                     SKNQKVKYFLLADIDPNFSIIKETHEDFYGNLSNRMRYIVRRGEIVTAKGGSATGTKG
                     HATALITEKFDGLVTTDALYNLVPRRINPYYLLFLFKQPIILNQVNMFTKGTLYKLIQ
                     RNDFEKIKIPRLESSLEEQIVDKMMNYLSVLQNKF*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaattgtg
      181 tgtttatcac cccggacaaa atggatggca aaagaatcag ctatgattac gtggtccagc
      241 agtatgaaat tcagcggttg ttacaacgtc gccctctgat tacgttaatc gatatatgca
      301 aggaaattac gtccggcatt cgcgtaaaaa aagagtatta tactgacaaa aatgggtata
      361 aaataattgc accaggggac atccgtaatg aagtaattta cataaatgag ttgaaagtcg
      421 tacagcccga ggtcgttcgt gaaaaagata taatcaacaa cggcgatatt ctgatcaccg
      481 caagtggtaa gagcggtcag gtcatttacg tgaacgaagt gcttgaaggc tgtgtcgtta
      541 cttcggatat tattaaaatt accctgagag accgtgacaa aggtatccgg ttgtataaat
      601 ttttaaaaag ttccatcggc caaatgctgc tgaactcaat taaaattggc atattaaaca
      661 agatttttgt cgaagacgta gagaatcttc tgatccctga ggattttgat acatatcagg
      721 aggactgctc ggacgattcc actgtatatg cggaagcaga gaagttgtac cgtagtgctg
      781 aaaacatatt ttaccgtgtg tttgactaca aaggtgaaaa aaaaaatctt aaacattttt
      841 acgtaaccga gtacctggat agtcatcgct tggatcctga atattattcg aatttttata
      901 ccgagcttta tcgggttata cataagaatt ttgacgatgt taaatgggag gaattaggcg
      961 agttagttga gataaaaaaa gcagataaac cggaaattag caaaaaccaa aaagttaaat
     1021 atttcttgct ggctgatatc gatcccaatt tcagtatcat taaggaaact cacgaagatt
     1081 tttatgggaa tcttagtaat cggatgcgtt acattgtaag acgtggcgaa attgtcacag
     1141 ccaagggggg atcagcaacg ggtacgaaag gtcatgctac cgccctgatt actgaaaagt
     1201 ttgacggact tgtgacgacc gatgcattat ataaccttgt tcctagacgt attaaccctt
     1261 attacctgtt atttctgttt aagcaaccaa ttatcctgaa tcaagtgaat atgtttacta
     1321 agggtacctt atataaatta attcaaagaa acgacttcga aaagatcaaa atcccccgcc
     1381 ttgaatcatc tctggaagaa caaattgttg acaagatgat gaattatctg tcggtactgc
     1441 agaataaatt ttga
//
