LOCUS       New Construct      3288 bp    DNA         SYN       30-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..2045
                     /gene="Fisuc1346"
                     /codon_start=1
                     /translation="MDNKSLKKLEADLWESADLLRADSKLTSNQYCMPVLGLLFLRYA
                     YGRFKKVEAELLKNRPSRGGRVLPIEASDFVSKSALFLPKEAQFDYLVNLPANIASAK
                     LKNVHGQPLNSLGEVVNNAMELVEAQSEKLKGVLPKTYTDFKDNLLAELLRTFNNNAL
                     DDVGGDVMGRIYEYFLSKFAKNIASDDGVFFTPKSLVKMIVNVLEPSYGVLLDPACGS
                     GGMFVQTGDFVNAAGMASNETMTFYGQEKVEYNAQLCLMNMAVHGLTGVVKSGDEANS
                     FYHDAHNLTGQCDYVMANPPFNVDKVKAESCQSAGRLPFGLPAVNKDKEIGNGNYLWI
                     SYFYSYLNEKGRAGFVMAASATDSQSKDKDIREKLVATGHVDCMISVGNNFFYTKSLP
                     CTLWFFDKCKKKNIKDKVLFIDARNYYTVVDRTLNEWSEWQLKNLNAIVWLYRGELDK
                     YQSLLAEYRTTLLSYADETDDANLEKILRKTSKIAKQVELLKEYRETLRAEAKASVDA
                     ADKKSKKKTQEEWDTRLAEFDEILNVAKEADWLYEKFGDGKYKDILGLCKIATRAEIA
                     EKNFSLTPGAYVGVAPVEDDGVDFAERMHEIHEELLKLQDESNTLMKTISKNMKELGI
                     *"
     misc_feature    2046..2061
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             2062..3288
                     /gene="Fisuc1345"
                     /codon_start=1
                     /translation="MAWEKVKLGDVCVSIADGDHLPPPKADCGIPFVTISNITSANQF
                     DFTNTMFVPQEYYDSLDEKRKPKVNDILYSVVGSFGKPVFIKDDSPFVFQRHIAILRP
                     DESKIYSRYLYYKMLSNDFYMMADAVAVGAAQRTVSLTALRNMEINIPNKETQKRIAD
                     ILSAYDDLIENNQKQIKLLEEAAQRLYKQWFIDLKFPGYETTPIVDGLPQGWWKEKLG
                     DVVDYVRGTSYTSNELSDNEGVLLVNLKNINAFGGYKRNAEKRFTGKYKENGILESGD
                     LVMGCTDMTKERRLVGHVALIPNLKECAIFTMDLLKILPKTISKTFFYAQCRFGGLSY
                     KISPLANGANVLHLRPENMADIEVLCPEKSIVEMYDNVFASMISKIEKLEDQIQLAAE
                     SRNRLLPKIMNGEISV*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atggacaata
      181 agagcctgaa aaaactggaa gcggatctgt gggaatcggc agacctgttg cgcgcggatt
      241 ccaagttaac ctcgaatcag tattgcatgc cagtacttgg gttactgttt ttacgttacg
      301 catatggccg ttttaagaaa gtcgaggccg aactgttaaa aaatcgcccg tcacgcgggg
      361 gaagagttct gccgattgaa gcgtcagatt tcgtgtccaa atctgcgctt tttcttccga
      421 aagaagcaca atttgattat ttggtaaatc tgccagctaa tatcgcgagt gcgaaactga
      481 aaaatgtgca cgggcagccg ctgaattctt taggggaggt agtgaataat gcaatggaac
      541 tggtcgaggc tcagtcagag aagctgaaag gcgttcttcc caaaacatat acagatttta
      601 aagataatct gttggcggag ttacttcgca catttaataa taacgccctg gatgatgtcg
      661 ggggtgatgt gatgggtcgc atctatgaat attttctgag caaattcgca aaaaacatag
      721 caagcgatga cggtgtcttt ttcactccga aaagccttgt aaaaatgatc gttaatgtgc
      781 ttgaaccttc ctatggagtt ttgcttgacc cggcttgtgg tagtggaggc atgtttgtcc
      841 agaccggtga ctttgtcaat gcggcgggaa tggcatcaaa tgagacgatg acattctatg
      901 gtcaggaaaa ggtggaatac aatgcacaac tgtgtctgat gaacatggca gtccatggtt
      961 taaccggggt tgttaagagt ggagatgagg caaactcctt ttatcatgac gcgcataact
     1021 taacgggtca gtgcgactac gttatggcga atcctccgtt caatgtagac aaagtgaaag
     1081 cagagagttg ccagtcagcg ggccgtctgc cgtttggctt acctgcagta aataaggaca
     1141 aagaaatcgg taatggaaat tacctgtgga ttagttactt ttatagttat ttaaacgaaa
     1201 aaggccgtgc ggggtttgtt atggctgcct cagcaactga ttctcaaagc aaagataaag
     1261 atattcgtga gaaattggtt gctacaggtc atgtggattg tatgatctcc gtcggtaata
     1321 attttttcta taccaagtcc ctgccgtgca cactgtggtt ttttgacaag tgtaaaaaaa
     1381 aaaatatcaa ggacaaagtt ctgtttattg atgcccgtaa ttactacacg gtcgttgatc
     1441 gtaccctgaa tgagtggtct gagtggcagt taaagaacct gaatgccatt gtgtggctgt
     1501 acagaggtga gctggataaa taccaatcac tgctggctga gtaccgtacc actctgcttt
     1561 cttatgcgga tgaaacagat gatgcaaact tagaaaagat cctgcgtaag acgtcgaaga
     1621 tagccaaaca ggttgaatta ttaaaagaat accgcgaaac gcttcgcgca gaggccaaag
     1681 catctgtaga cgcggcggac aaaaaatcta agaaaaagac acaagaagaa tgggacacac
     1741 gcttagcaga atttgatgaa atcttgaacg tagctaaaga agcagactgg ctgtatgaaa
     1801 agtttggaga tggcaaatat aaagacattc tggggttgtg taaaatcgcg acgcgcgcag
     1861 agattgctga aaaaaatttt tctcttacgc ccggcgcgta tgtgggcgta gctccagttg
     1921 aagatgatgg cgtcgatttc gccgaacgca tgcatgaaat acatgaagaa ttattgaaac
     1981 tgcaggatga gagtaataca ctgatgaaaa caatttcaaa aaatatgaaa gagctgggca
     2041 tttaaatagg aggtgttcaa tatggcatgg gaaaaagtta agctggggga tgtctgtgtc
     2101 tctattgcgg acggcgatca cttgcccccg ccgaaggccg attgtggaat acccttcgtt
     2161 acgatttcga acatcacatc cgcaaatcag tttgacttta cgaacacaat gttcgttcca
     2221 caggagtact atgattctct tgatgaaaaa cgcaaaccga aggttaacga tatcttatac
     2281 agcgttgtgg gctctttcgg aaaaccggtg ttcattaaag atgattcccc attcgtcttt
     2341 cagcgccaca tcgccattct gcgtccggac gaatcgaaaa tttattcccg ctatttatat
     2401 tataagatgt tgagcaatga cttttatatg atggcggatg cagttgcggt gggtgcggcg
     2461 cagcgcactg tttctctgac cgcattgcgc aatatggaaa tcaatattcc gaacaaagaa
     2521 acccaaaagc gcattgcgga tatcctgtct gcgtacgatg atctgattga gaataatcag
     2581 aaacagatta aattactgga ggaagcggca cagagactgt ataaacagtg gtttattgac
     2641 ttaaagtttc ctggatatga gactaccccg atcgtggatg gattgccaca aggatggtgg
     2701 aaggaaaagt taggtgacgt tgtagattat gttcgcggaa ccagttatac ctccaacgaa
     2761 ttgagtgata acgaaggagt cctgcttgtg aatctgaaaa atatcaatgc gttcggaggt
     2821 tacaaacgca atgccgaaaa gcggtttact ggaaaataca aggaaaacgg aattttggaa
     2881 tctggtgatc ttgtgatggg ctgtaccgat atgacgaaag aacggagact tgtgggacat
     2941 gtagccttaa ttcctaatct gaaggaatgt gccatcttca ctatggacct gctgaaaatt
     3001 ttacctaaaa cgattagtaa gacgtttttc tatgcccaat gccggtttgg tggtctgagc
     3061 tataaaatca gcccgctggc aaatggtgcc aacgttttac atctgcgtcc tgaaaatatg
     3121 gcagacatag aagttttgtg tccggagaaa agtatcgtgg agatgtacga caacgtgttc
     3181 gctagtatga ttagcaaaat cgaaaagctg gaagatcaga ttcagttagc cgcagaaagc
     3241 cggaaccgcc tgctgccgaa gatcatgaat ggagagatct cagtctaa
//
