LOCUS       New Construct      2784 bp    DNA         SYN       30-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1616
                     /gene="Fisuc1722"
                     /codon_start=1
                     /translation="MFEQTFNNIDDILWKEGGCSNELDYVEQTSWVLFLKYLDNLEAE
                     REEEAELKGETYERIIDKKFRWNTWAAPKTKNGEPDHAKALTGDDLTDFVNNKLFPYL
                     KKFKETATTPQSLEYKIGEIFGELRNKITSGYNLREILWYADALSFQSSEDKHEMSHL
                     YEDKIRRMGNAGRNGGEYYTPRPLIRTIVRIIDPKIGETVLDPACGSAGFLCEAYAYM
                     KQKVKSVADRETLQKKTFYGQEKKGLAYIIGIMNMILHGVNAPNILHTNTLSENMANV
                     EQKMRKDVIIANPPFGGKERAEVQQNFPIKTSETAYLFMQYFVKLLKAGGRAGIVIKN
                     TFLSNTDNASVMLRKELLENCDLHTILDLPSGVFTGAGVKTVVLFFEKGRPTEKIWYY
                     QPDFGRNLGKTNPLTEDDLAEFVKLQKKKTDSEKSWTINVKDLNPETYDLSVKNPNKK
                     TEVELRDAKTIIKEMQSLDEENRKILAKLSI*"
     misc_feature    1617..1632
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1633..2784
                     /gene="Fisuc1721"
                     /codon_start=1
                     /translation="MSKWEWKKLKDICEKGSSNIKQSDLKDLTGDYPIFGASGYIQNV
                     DFYQRNRDYIGIIKDGSGVGRTMLLPAFSSVIGTLQYILPKEGNDIKFINYALQNIDF
                     SKSIQGAAIPHIYFKDYGETEILVPPLSEQKSIVKFLDEEFSKIETLKTNAETNLKNA
                     KELFESTLEKELNPGKNGTLPSGWEWKTLRELCILRPSKNEALSHLKGTDEVSFLPME
                     DLNIRERNTIPHKSRALSEVHGSYTFFAEGDVLLAKVTPCFENGKMGIASNLLNGVGF
                     GSSEYIVFRTTKSMINSYLFYVLMSSRFISGGKKQMLGACGLKRLSKEYVESFQIPLP
                     PLSVQKEIVARLDKLSENVKRLEVNYKQIIANCDELKKSILKKTFEGES*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgttcgagc
      181 agacctttaa taatattgac gacatactgt ggaaagaagg aggttgttca aacgaactgg
      241 actatgttga acagacatcg tgggttcttt ttctgaaata tttagataat ttagaagcag
      301 aacgtgaaga agaagcggaa ttaaagggcg aaacatatga aagaatcatc gacaaaaaat
      361 tcagatggaa tacctgggca gccccgaaaa cgaaaaatgg agagccggac catgcaaagg
      421 cactgacagg agatgatctg actgacttcg tcaataacaa acttttcccc taccttaaaa
      481 aattcaaaga aactgctact actcctcaga gtcttgagta taaaattggc gagatttttg
      541 gagaactgcg caataaaatt accagtggtt ataacctgcg cgagattttg tggtatgcgg
      601 atgccctgtc attccagtca tcggaggata agcacgaaat gagtcatctg tatgaagata
      661 aaattcggcg catgggcaat gctggccgca atggtggtga atattatacc ccgcgtccat
      721 taattcgtac catagttcgg attattgacc cgaaaattgg ggaaacagtc ctggatcccg
      781 cgtgtggttc tgcgggtttc ctttgcgagg cttacgctta catgaaacaa aaggtaaaga
      841 gtgtggccga tcgcgaaacg cttcaaaaaa agacgttcta tggccaagag aaaaaaggcc
      901 tggcttacat tatcggcatt atgaatatga ttttgcacgg cgtaaatgcg ccaaacatcc
      961 tgcacacgaa caccttgtct gaaaatatgg cgaatgtgga gcaaaagatg cgcaaagacg
     1021 ttataatagc taaccccccg ttcgggggta aagaacgtgc agaagtccaa cagaatttcc
     1081 caatcaaaac tagtgaaacg gcctatttgt tcatgcaata ttttgtcaag ttgttgaaag
     1141 caggcggacg cgcagggatt gtcataaaaa atacgttcct gagtaataca gacaatgcga
     1201 gtgttatgct gcgtaaagaa ctgctggaaa attgcgactt gcataccatt ctggatctgc
     1261 ccagcggcgt ttttaccggt gcaggtgtga aaactgtggt gttattcttt gagaaggggc
     1321 ggcctaccga aaaaatatgg tactatcaac cggattttgg tagaaacctg ggcaaaacta
     1381 atcccctgac agaagatgac ttggcagaat ttgtcaaatt acagaagaaa aagacggata
     1441 gtgagaaatc ctggactatt aacgttaaag acctgaatcc tgagacctat gatctgtcgg
     1501 taaagaatcc gaataaaaaa acagaagtcg agctgcgtga tgccaaaacc attataaaag
     1561 agatgcaatc tcttgatgag gaaaaccgta aaattttggc aaagttatct atataaatag
     1621 gaggtgttca atatgagcaa atgggaatgg aaaaagctga aagacatctg tgagaaaggt
     1681 tcttctaata tcaaacagtc tgatttaaaa gatctgaccg gagactatcc aatcttcggc
     1741 gcaagcggct atatccagaa cgtggatttt tatcaaagaa accgggacta catcggcatt
     1801 attaaagatg gtagcggtgt gggtcgtacc atgttgcttc cagcgttcag ttcagtgatc
     1861 gggaccctgc agtacatact gcctaaagaa ggcaatgaca taaaattcat caattatgcc
     1921 cttcaaaata ttgatttttc aaaaagtatc cagggggcgg ctattcctca tatttacttt
     1981 aaagattatg gtgaaacaga aattctggtt ccgccgctgt ctgaacagaa gtccattgta
     2041 aaatttcttg acgaagaatt tagtaaaatt gaaaccttga aaacaaacgc tgagacaaat
     2101 ctgaaaaatg cgaaggaatt gttcgagtcg acgctggaga aagaattaaa tcccggtaaa
     2161 aatggaacac tgccctcagg gtgggagtgg aagactctga gagaattgtg tatcttgcgc
     2221 ccttcgaaaa atgaagcact gtcgcatctg aagggtaccg atgaagtttc ctttttacca
     2281 atggaagatc ttaacattcg tgagcggaat acaattccac acaaatcccg ggccctgagc
     2341 gaagtacatg gatcttatac gtttttcgct gaaggcgatg tgttactggc aaaagttact
     2401 ccctgttttg aaaatgggaa aatgggaatt gcttctaact tattgaacgg tgtgggtttc
     2461 ggctctagcg agtatattgt ttttcgcact acgaaatcca tgataaattc ttatctgttt
     2521 tatgtattga tgtcaagtcg ctttatttcg gggggcaaaa agcaaatgct gggagcctgt
     2581 ggactgaaac gcctgagcaa ggaatatgtt gagagttttc agataccgtt acccccctta
     2641 tccgtccaaa aagaaatagt tgcgcggctg gacaaactga gcgaaaatgt taaacgcctt
     2701 gaagttaact ataagcagat aatagccaac tgtgacgaat tgaaaaagag tattctgaaa
     2761 aaaacttttg agggagaaag ctaa
//
