LOCUS       New Construct      2819 bp    DNA         SYN       30-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..2819
                     /gene="Fisuc2448"
                     /codon_start=1
                     /translation="MPSIAEIENKIEDIVAKYRETGATGDFIYDFILAYDLPKASVKR
                     LQNGSLNLSKNQGEICWKGKLLYKELFKDELNVSLALETAKSIKHNERFVVLSDGKKL
                     LCVDTKTKDTLETEFKDLPKHSSFFLPWAGIEKTEFIDENPADVRAARKMAKLFDEIK
                     KDNAKKLISQHDFNVFLSRLLFCFFAEDTGIFTDNLFTNTIAATTKADGSDLNEFLER
                     LFTVLDTAPKERKKLPDYLEKFPYVNGGLFRNKIEIPRFSAHSRNKIIECGELNWKDI
                     NPDIFGSMFQTVIDEEQRGDLGQHYTSVPNIMKVIEPLFLNDLRKEFDAAGKSVTKLK
                     NLLLRIRNIQIFDPACGSGNFLIIAYKELRKLEMDILLKMQEFGLTGIHLNHFYGIEI
                     DDFACETAKLSLWLAEHQMNVEFFSKTGTICPTLPLKEAGHIVCDNACNIDWNNVCPN
                     NGNDEIYVLGNPPYLGARLQKENHKKDVEAVFENVKGAKNLDYIACWFYKGSDYIANS
                     NATLAFVSTNSICQGEQVAILWPQIFNKGIEIQFGYSSFKWTNNAQYNAGVIVTIIGL
                     SDEKLRKEHFLFNGLRKNKASKINAYLSSGSDVIVEEKKDSLSDFPPMNFGSMPNDGQ
                     YLLFEKKEHDDAVKKCPEIKKFMKLFLGADEFINGEKRYCLWITDNSKKEAMSIPIIA
                     ERVKKCKELRVKSKRAATNKLALVPYKFGEIRYKETSSIIVPGASSERRTYIPMDFLS
                     KDTVVSNAAFAVYDAEPWLFSILNSKMHMAWVRTVGGKLKNDYRYSAKLVYNTFPFPH
                     INDEQKNMLKDYAFGIIAARENHPGKTLAELYNPESMPNDLKKAHEENDFAIEKIYRA
                     RPFANDEERLEFLFRLYEQMIREEQE*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgccgagta
      181 tagcggaaat agaaaacaag attgaagaca tcgtggctaa atatcgcgag acaggcgcaa
      241 ctggcgattt tatctatgac ttcattctgg cctacgattt gccaaaagcg agcgtcaaac
      301 ggctgcagaa tggttcattg aatctgtcaa aaaatcaggg ggaaatctgc tggaagggca
      361 aactgctgta taaagaatta ttcaaagatg aattgaatgt gagcctggct ttagaaactg
      421 ctaaaagtat taaacataat gagcgtttcg tggtcctgtc tgatggtaag aaactgttgt
      481 gcgttgatac aaaaacgaag gataccttgg agacggaatt taaggatctg cccaaacact
      541 caagcttctt cctgccgtgg gccgggatcg aaaaaacgga atttatagat gaaaatcccg
      601 cggacgttcg cgcggcgcgc aagatggcta aactgttcga tgaaattaaa aaagataatg
      661 caaaaaaact gatttctcaa catgatttca acgtgtttct gtcacggtta ttgttttgct
      721 tctttgctga agacaccggt atctttacgg ataatctttt tacgaataca atagcagcga
      781 ctacgaaagc tgatggatct gacttgaatg aatttctgga acgtttgttt acggttttgg
      841 atactgcacc aaaagagcgt aaaaaacttc ctgattatct ggaaaaattt ccttatgtga
      901 atggtggtct gtttcgtaat aaaattgaga ttccgcggtt tagcgcgcat agccgcaaca
      961 agattattga atgtggtgag cttaattgga aagacataaa tcccgatata tttggttcta
     1021 tgtttcagac cgtaattgac gaggagcaac gcggcgattt ggggcagcat tacactagcg
     1081 tgccgaatat tatgaaagta attgaaccac tgttccttaa cgatctgcgg aaagagttcg
     1141 acgccgccgg taagtcggtg actaaattaa aaaatctgct tctgcgcatt agaaatatcc
     1201 aaatcttcga tccggcatgt ggtagcggta actttctgat tatcgcttat aaagaacttc
     1261 ggaaactgga aatggacatc ttacttaaaa tgcaggagtt tggattaaca ggtattcatc
     1321 tgaaccactt ttatggcatt gaaattgatg atttcgcttg tgagaccgcc aagttgagtt
     1381 tatggctggc agaacaccag atgaatgttg aattcttctc taaaacaggt actatttgcc
     1441 ctaccctgcc attaaaggaa gcggggcata ttgtgtgcga caatgcatgc aatatcgatt
     1501 ggaataacgt ttgcccgaat aatggcaatg acgagatcta tgttttagga aatccgccat
     1561 acctgggtgc gagactgcag aaggaaaatc acaaaaaaga tgtcgaagca gtattcgaga
     1621 atgtcaaagg cgctaaaaat cttgactaca tcgcctgctg gttctataaa ggttcggatt
     1681 acattgcgaa ttctaatgcc actttggctt tcgtaagcac caactccatc tgccagggtg
     1741 agcaggtggc aatcttatgg ccccagatct ttaataaggg cattgaaata caatttggct
     1801 attcctcttt taagtggact aataatgcgc agtacaacgc gggagtgatt gtaactatta
     1861 tcggtttatc cgatgaaaaa cttcggaaag aacactttct ttttaacggt ctgcgcaaaa
     1921 acaaagcaag caaaataaac gcatatctgt cgtcaggatc tgatgtgatt gttgaggaga
     1981 aaaaagacag cctttcagat tttccgccta tgaactttgg ttcaatgccg aatgatggcc
     2041 agtatttgct gtttgagaaa aaagaacatg atgacgcggt aaagaaatgc ccggaaatca
     2101 aaaaattcat gaaactgttt ttgggtgctg acgaatttat caatggtgaa aagcgttatt
     2161 gtctgtggat taccgacaac tcaaaaaaag aggcaatgtc aataccaatc attgcggagc
     2221 gcgtgaaaaa gtgtaaagaa ctgcgcgtaa aatcgaaaag agcggctaca aataagttag
     2281 cacttgtccc ctacaagttc ggcgagattc gttacaaaga aaccagctcc atcatcgttc
     2341 caggggcctc gtcagaacgt agaacctaca ttccaatgga ttttctgagt aaagacacgg
     2401 tggtgagcaa cgctgctttt gctgtgtatg atgcggagcc ctggttattt tccattctga
     2461 actcgaaaat gcatatggca tgggtaagaa cagtgggcgg gaagctgaaa aacgactata
     2521 gatattctgc taaactggtt tacaatacct ttccgtttcc acatataaac gatgagcaga
     2581 aaaacatgtt aaaggattac gcctttggaa tcatcgctgc tcgggaaaat catccgggta
     2641 agaccctggc ggaactgtac aatcccgagt cgatgccgaa cgatttaaag aaagcccatg
     2701 aagaaaatga ttttgcaatt gaaaaaattt atcgtgcccg cccatttgct aacgatgaag
     2761 aacgtttaga atttttgttt agattgtatg aacagatgat cagagaagaa caggaataa
//
