LOCUS       New Construct      1154 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1154
                     /gene="M.HhaI-GCGC"
                     /codon_start=1
                     /translation="MIEIKDKQLTGLRFIDLFAGLGGFRLALESCGAECVYSNEWDKY
                     AQEVYEMNFGEKPEGDITQVNEKTIPDHDILCAGFPCQAFSISGKQKGFEDSRGTLFF
                     DIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIP
                     QKRERIYMICFRNDLNIQNFQFPKPFELNTFVKDLLLPDSEVEHLVIDRKDLVMTNQE
                     IEQTTPKTVRLGIVGKGGQGERIYSTRGIAITLSAYGGGIFAKTGGYLVNGKTRKLHP
                     RECARVMGYPDSYKVHPSTSQAYKQFGNSVVINVLQYIAYNIGSSLNFKPY*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgatcgaaa
      181 taaaagataa acaactgact gggctgcgtt ttattgacct tttcgcgggg ttaggtggat
      241 tccgtttggc actggagtca tgcggagcag agtgtgtata tagtaatgaa tgggataagt
      301 acgcccaaga ggtgtacgaa atgaactttg gcgagaaacc agagggggat ataacccagg
      361 ttaacgaaaa gactatacct gaccatgata tcttgtgtgc aggatttccg tgtcaggcgt
      421 tttccattag tgggaagcag aaaggctttg aggatagtcg tgggactctg ttcttcgaca
      481 tagctcgcat cgttcgcgaa aaaaaaccga aggtcgtttt catggaaaat gttaaaaatt
      541 ttgcatccca tgacaatggt aatacattgg aagtagtaaa gaacactatg aacgagctgg
      601 attactcttt tcatgctaaa gtcttaaacg cgttggatta tgggattcca cagaagcgcg
      661 agcgtatcta tatgatatgt tttcgcaatg acctgaacat acaaaacttt cagttcccga
      721 agccatttga gttgaataca tttgtcaaag acctgctgct gccggattcg gaagtcgaac
      781 acttggttat tgatcgtaaa gatttagtta tgactaacca ggaaatcgag cagactactc
      841 cgaaaaccgt acgtcttggc attgtcggta agggcggaca gggggaacgt atttacagta
      901 cccgcggcat tgctatcact ctttctgctt acggtggtgg tatattcgcc aaaacaggtg
      961 gctaccttgt taatggaaaa acaagaaaac tgcatccgcg tgagtgtgct cgcgtaatgg
     1021 gttacccaga cagttataaa gtacatccaa gcacgagcca ggcgtacaaa cagtttggta
     1081 acagcgtcgt gatcaatgtg ttgcagtaca tcgcatataa tataggtagt agccttaact
     1141 tcaagccata ctaa
//
