LOCUS       New Construct      1154 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1154
                     /gene="M.LmoJ3"
                     /codon_start=1
                     /translation="MKYRAISLFAGVGGLDLGFEQTGKFETIWANEFDEKAALTYRSN
                     FNNTLSTLDIHEVNKKELPKANIVLSGFPCQAFSVAGYRLGFEDERGDLFFETLKVIN
                     SVNPEVVFLENVKNLVGHDKGRTFRIIFDALEQHGYFVKYQTLNAKDYGNIPQNRERI
                     YIVGFRNKSAFEKFDFPKPIELKKKLSDVIDYDSQLEDRYYYSPEKHTFYDLLEQDIV
                     SQDTLYQWRRQYVRENKSNVSPTLTANMGTGGHNVPLILSKHGIRKLTPRECFNLQGY
                     PPSYILPNIAISHLYKQAGNSVVVPVIKRIAENISMALEEASKIDLIQLDI*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaagtatc
      181 gcgccattag cttgttcgcg ggcgttggtg gtttagacct gggctttgag cagacgggga
      241 agtttgaaac gatatgggct aatgaatttg atgaaaaagc agcactgaca tatcgctcta
      301 actttaataa caccctttcc acccttgata ttcatgaagt caataaaaaa gagctgccta
      361 aagctaatat tgttctgtct ggattcccct gtcaggcgtt ctctgttgcc ggctaccgcc
      421 ttggattcga ggacgaacgt ggagatctgt tcttcgaaac tttaaaagtg atcaattcgg
      481 tcaacccaga agttgtgttt ttagaaaacg tgaagaactt agtgggtcac gataagggca
      541 gaacattccg cataatcttt gacgcgcttg aacagcatgg atatttcgtg aaatatcaga
      601 cattaaatgc taaagattat ggcaatattc cccagaatcg cgagagaatc tatattgtag
      661 gctttagaaa taaatctgcc ttcgagaaat ttgattttcc caaaccgatc gagttaaaga
      721 aaaagttaag tgatgtcatc gattatgact cgcagctgga agatcggtat tattatagtc
      781 cggagaaaca caccttttat gacttattgg agcaggatat cgtgtcccag gatacacttt
      841 accagtggcg gcgtcagtac gtcagagaga ataagtccaa tgttagcccg acgcttactg
      901 ctaacatggg gaccggtggc cacaatgtgc cattgatact ttcaaaacat gggattcgta
      961 aattgacccc gcgtgagtgt ttcaacctgc aggggtatcc gccgagttat attctgccta
     1021 acatcgcgat tagtcatttg tacaaacaag ccggaaactc cgtagtcgtg cctgtcataa
     1081 aaagaatcgc tgaaaatatt tcaatggcac tggaggaagc gtcgaaaatc gacttaattc
     1141 agttagatat atga
//
