LOCUS       New Construct      1313 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1313
                     /gene="M.LmoJ2"
                     /codon_start=1
                     /translation="MIFRTAELFAGPGGGAYAAKTAKYVDREGNNWGFEHAWANEYDK
                     DTVETYKLNILGDPEATTVYCQDVRKFDLTNHKLLSDIDALIFGFPCNDYSLVGKQKG
                     LDGEFGPLYSYGSKALSEFQPKVFIAENVGGLSSANEGEAFIHILKEFQKAGYKLTPH
                     FYKFEQYGVPQARHRIIIVGIRNDIYEEGITFRVPAPIFKDHTEYKKAYDALTNPPIS
                     LNALNHEFTKHNAKTEKMLSYIPEGGNAWVESIPEAFRLNVKGARLSNIYKRLHRNKP
                     SYTVTGSGGGGTHMYHWSENRALTNRERARLQTFPDDFYFQGGKESVRKQIGMAIPPD
                     GLRPILLAVLKSFAQEDYEWIEPTKKLQTDILFNESGKTIATIVKS*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgatcttcc
      181 ggaccgccga gttatttgct ggccctggtg gaggtgctta cgccgctaaa actgctaaat
      241 atgtcgaccg cgaaggaaac aactgggggt ttgagcatgc ctgggctaat gagtatgata
      301 aagatacagt agagacctac aagctgaata ttctgggaga cccggaagcg actactgtct
      361 actgtcagga tgttcgcaaa ttcgatttga ccaatcataa actgctttcg gatattgacg
      421 cactgatatt cgggttccct tgtaatgatt atagtctggt cggcaagcag aaaggtctgg
      481 atggtgagtt tggtccactt tattcttatg gcagtaaagc actgtctgaa tttcaaccca
      541 aagttttcat cgctgaaaat gtgggtggtc tgtcctcagc caatgaggga gaggcattca
      601 ttcatatctt gaaagagttt caaaaggcag ggtataaact tacaccccat ttctacaaat
      661 ttgaacagta cggggtcccg caagccagac accgtataat aatagtcggc attcgtaatg
      721 acatctatga agaggggatt acatttcgcg tgcccgcgcc catttttaaa gatcatactg
      781 aatataaaaa ggcctatgac gccttaacca acccgccgat tagccttaac gctctgaatc
      841 atgaattcac aaaacataac gcaaaaacgg aaaaaatgtt gagttacatc cccgaggggg
      901 gaaatgcgtg ggttgaaagc attccggaag cgtttcgtct gaatgtgaaa ggtgccagac
      961 tgtcaaacat atataagcgg cttcaccgca acaaaccgag ttacacggtt accggatccg
     1021 gagggggtgg tacacacatg tatcattggt ccgaaaaccg tgcgctgacg aatagagaac
     1081 gcgcgcgcct gcagactttt ccggatgatt tttactttca gggcggaaaa gaatccgtgc
     1141 ggaaacagat tggcatggcc attccgcctg atgggcttcg ccctatcctt ctggctgtgt
     1201 taaaatcatt cgcacaagaa gattacgagt ggatagagcc gacgaaaaag ttgcaaaccg
     1261 atatcttatt caatgaatct ggtaaaacca tcgccacgat tgttaagtcc tga
//
