LOCUS       New Construct      1163 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1163
                     /gene="M.HaeIII-GGCC"
                     /codon_start=1
                     /translation="MNLISLFSGAGGLDLGFQKAGFRIICANEYDKSIWKTYESNHSA
                     KLIKGDISKISSDEFPKCDGIIGGPPCQSWSEGGSLRGIDDPRGKLFYEYIRILKQKK
                     PIFFLAENVKGMMAQRHNKAVQEFIQEFDNAGYDVHIILLNANDYGVAQDRKRVFYIG
                     FRKELNINYLPPIPHLIKPTFKDVIWDLKDNPIPALDKNKTNGNKCIYPNHEYFIGSY
                     STIFMSRNRVRQWNEPAFTVQASGRQCQLHPQAPVMLKVSKNLNKFVEGKEHLYRRLT
                     VRECARVQGFPDDFIFHYESLNDGYKMIGNAVPVNLAYEIAKTIKSALEICKGN*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaatctga
      181 tttctctttt ttctggtgcc gggggcctgg atctggggtt tcagaaggca ggcttccgga
      241 ttatctgtgc caatgagtac gacaaatcta tttggaagac atatgaatca aatcatagtg
      301 caaagcttat caagggggat atttctaaaa ttagttcaga tgagtttcct aaatgcgatg
      361 gaattattgg tggcccaccg tgtcagtcat ggtcagaggg gggctccctg cgtgggattg
      421 acgacccgcg tggtaaactg ttctatgagt atattcggat tcttaaacaa aaaaaaccta
      481 ttttttttct tgccgagaat gtaaagggaa tgatggctca gcgtcataac aaggcggttc
      541 aagaattcat tcaggaattc gataatgctg gctatgatgt ccatattata ttgctgaacg
      601 ctaatgacta cggagtggcg caggatcgta aacgcgtctt ttatattggt ttcagaaagg
      661 agttgaatat taattacttg ccgccgatac cacacctgat taaaccgaca tttaaagatg
      721 taatttggga ccttaaagac aatccaattc cagcactgga taaaaataag accaatggca
      781 ataaatgtat atatccaaac catgagtatt ttattggttc atatagcacc attttcatga
      841 gccgtaaccg tgtccgccag tggaatgagc cggcattcac agtacaagcc agtggccgtc
      901 aatgtcagct tcacccgcag gcgccggtca tgttaaaagt aagtaaaaat ctgaacaagt
      961 tcgtagaagg taaggagcac ctgtatcgtc ggcttacagt aagagaatgc gcacgcgttc
     1021 aaggattccc cgatgatttt atttttcact acgaatctct taatgatggt tataaaatga
     1081 ttggcaatgc ggtgccggtt aacttagcct atgagattgc aaaaaccatc aagagcgcgc
     1141 tggaaatctg caaagggaat taa
//
