LOCUS       New Construct      1556 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1556
                     /gene="M.SinI"
                     /codon_start=1
                     /translation="MIMNDIITVTEAAQLLELTPQRVRTMCKQGSIDAYQSGRTWLIK
                     SSSVEKLMLVNSLSDAQNSYSMLASEPKNKPKALSFFSGAMGLDLGIEQAGFETLLAS
                     EIDKAARDTILSNRPNMALIGDIRDYTTEDILKLAGVSSGNEIDLIMGGPPCQAFSTA
                     GKRLGLEDERGNVFIKYLDVALDIRPKYIVIENVRGLLSAPMKHRPHNERGEGLPPLK
                     SEEQPGGVLHYIIRIIKSAGYSVSFNLYNSANFGVPQIRERVIIICSRDGSRVPFLQP
                     THSEKGEYGLPKWITLRETITNLKNITHEHVLFPEKRLKYYRLLKEGQYWKHLPEDLQ
                     KEALGKSFFLGGGKTGFLRRVAWDRPSPTLVTHPAMPATDLAHPDELRPLSVQEYKVI
                     QQFPEEWVIKGKLLDKYRQLGNAVPIGLGLAVGKNILDHMNGRKIESFPNFRYSRYKN
                     TSDLDIFGELV*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgattatga
      181 atgacattat cactgttacc gaagccgcac agcttttgga gcttactcca caacgggtgc
      241 gtaccatgtg taaacagggc tctattgatg cttaccagtc tggtcggaca tggttgatta
      301 aaagtagttc agtggaaaaa cttatgctgg taaactctct gagcgacgct cagaactcat
      361 acagcatgct ggcttctgaa ccgaaaaata aaccgaaagc attgagcttc ttcagcggtg
      421 caatgggtct ggacttaggt atagaacaag cgggctttga aactctgttg gcttctgaaa
      481 tagataaagc ggcgcgcgat accatactgt caaatcgccc gaatatggcg ttaatcggcg
      541 atataagaga ttatacgaca gaagatatac ttaaattagc tggcgtttcg tcggggaatg
      601 agatcgatct gatcatgggc ggaccgccgt gccaggcttt ctcaactgcc gggaaacgtc
      661 tgggtctgga agatgagaga ggtaacgtgt ttattaagta cctggatgtc gctttggata
      721 tccgtccgaa gtacatcgtt atagagaacg tgcgcgggtt actttccgct ccgatgaaac
      781 atcggccgca taacgagcgt ggcgaaggtc tgcctccgtt gaaatctgaa gaacaacctg
      841 gcggcgttct gcactatata attcggatta tcaagtctgc aggctatagt gtttcattta
      901 atctgtataa tagtgcaaat tttggcgtac cgcagatccg cgaaagagta ataatcattt
      961 gtagtcggga tggctcaaga gtcccgtttc tgcagccgac tcatagtgaa aaaggagagt
     1021 atggtttacc aaaatggatc accttacgcg aaacgattac caatcttaaa aatatcaccc
     1081 acgagcatgt cttatttcct gaaaagcgcc tgaaatatta tcgtttattg aaagaggggc
     1141 aatactggaa gcacttgcca gaagatctgc agaaagaagc tcttggcaaa tccttttttt
     1201 taggtggtgg gaagacaggt tttttgcgcc gtgtagcctg ggaccgccct tcgccgacac
     1261 tggtcaccca tccggctatg ccggcaacag acttagcaca cccggatgag ttgcgcccct
     1321 taagtgtaca ggagtataag gttatacagc aattccctga ggaatgggtg ataaagggta
     1381 aactgttgga taaataccgc cagctgggaa atgcggtgcc gatcggtttg ggtttagccg
     1441 tgggtaagaa tatcttagac catatgaatg gccgcaaaat cgagagcttt ccgaattttc
     1501 gttactcacg gtacaaaaac accagtgatc tggatatctt tggtgaactg gtatga
//
