LOCUS       New Construct      3963 bp    DNA         SYN       6-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..2678
                     /gene="h16_A0004"
                     /codon_start=1
                     /translation="MPLTLPQLERHLFKAADILRGKMDASEFKEYIFGMLFLKRCSDV
                     FDQRREEVIAERMNAGESRANAEKSAELERWYKGAEYFWVPPRSRYKFLLDEAHQNVG
                     DSLNKALSGIETANTKLYDVLEHIDFTRKVGQSKIPDIKLRQLITHFGIYRLRNEDFE
                     FPDLLGAAYEYLIGEFADSAGKKGGEFYTPRSVVRMMVRLIQPTLAHDIYDPCCGSGG
                     MLIAAKEYIDEHGEDGRKANLFGQEFNGTVWSIAKMNMLLHGISTANLQNEDTLAEPQ
                     HVEGGELMHFDRVLTNPPFSINWGNTEKNADGTPAWSPKFPERFRYGQVPLGAKKADL
                     MFLQHMLAVTRDGGMVATVMPHGVLFRGGEEKAIRAGIVEDDLLEAVLGVAPNLFYGT
                     GIPACILVLRQRVQNGANRVSGKPAERRGKVLFINADREFFEGRAQNHLLPEHIEKIV
                     TTFNEFKQVEGFSAIVDNATLKANDYNLNIRRYADNTPPPEPHDVRAHLVGGVPKAEV
                     QAKSTLFAAHGLNPMDLFVERDAKYVDFKPELAKRRDLKPSIERNAGLVAKEAAIRAA
                     FEQWWHQHSARITLLAGQMDNAASLVGLRNDLLASFSESLEAIGMLDPFQVRGIVAGF
                     WYQTKYDFLTLMARGAKGVADAWRTSIVTALEDKANKESPLDHKLVKFLMSDFVDAIN
                     ELEAKRAELDSQIKAADPKSGKDAEGDEGDAAESSEDEPADDNAVDETQLKAWKKELA
                     TAKKALKAKKDSFAAHLNREVDGLSPEQAAELLLTILHNDMRAIVERYIAAQRKAIVV
                     AFENWWDKYRVTLTEIEGKRDSAAQALQVFLKGLRYV*"
     misc_feature    2679..2694
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             2695..3963
                     /gene="H16_A0005"
                     /codon_start=1
                     /translation="MSNFVALGDHITVQKGKAPLVTGYVGKGAEPYLSPEYLRGRAPA
                     DLAKAGPDAVRAAEGETILLWDGSNAGEFFRSKVGLVASTMTKISPSSVFRPAYFFHV
                     AKQAERFLKAQTNGTGIPHVDRELLEGIKVFCPGSTEQQLLAEILDTLDTAIYETEAI
                     IAKLKAVKQGLLHDLLTRGIDANGELRPPQAEAPHLYESSPLGWIPNEWGLAPTATRC
                     HLITKGTTPAANEMWQGGAGIRFLRVDNLSFDGQLDLDASTFRVSLATHKGFLARSRC
                     LEGDVLTNIVGPPLGKLGLVTKEIGEVNINQAIALFRPTEQLLPKFLLIWLSSSISQS
                     WLRNRAKQTSGQVNLTLALCQELPLPRMTINEQQAIVDRVDAAQEQIWCEEELIRKMR
                     LEKSGLMDDLLTGRVRVKPQLAETKQAGSA*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgcctttaa
      181 ctttaccgca acttgagcgt cacttattta aagcagcaga tatcttaaga ggcaaaatgg
      241 atgcctcaga gtttaaagag tatatttttg ggatgctgtt cctgaaacgg tgctcggatg
      301 tgtttgacca gcggcgcgag gaggtaatcg cggaaagaat gaacgccggc gaatcccgtg
      361 caaacgccga aaaaagtgcg gagttagaga gatggtacaa gggtgcggag tacttctggg
      421 taccgccgcg ttcaagatat aaatttctgt tagatgaagc ccaccaaaac gtcggtgata
      481 gtctgaacaa agcactgagc ggcattgaga ctgcaaatac taagctttat gatgtactgg
      541 aacatatcga ttttactcgc aaagtaggtc aaagcaaaat tccagatatc aaactgcgcc
      601 agttgattac acattttggt atttatcgcc tgcgtaacga agatttcgag tttccagatt
      661 tattaggtgc ggcgtacgaa tatctgatag gcgagtttgc agacagcgcg ggaaaaaagg
      721 gaggtgaatt ttacacacca cgctcggttg tccgcatgat ggtgcgctta atccagccaa
      781 ctttggcaca tgatatttat gatccgtgct gcggttcggg tggcatgtta attgctgcaa
      841 aagaatatat tgacgagcat ggcgaggatg gtagaaaagc caatttgttt ggacaggaat
      901 ttaacgggac agtatggtct atcgcgaaaa tgaacatgtt actgcatgga atttccaccg
      961 ccaatcttca gaacgaagat actttagctg aaccccagca cgtggagggc ggcgaattaa
     1021 tgcatttcga ccgtgttctg accaatccac cgtttagtat taattggggc aatacggaga
     1081 aaaatgctga tggcactccg gcctggagcc cgaaatttcc ggagcgcttc cgttacggtc
     1141 aagtgcctct tggcgcgaaa aaggccgacc tgatgtttct gcagcatatg cttgcagtta
     1201 cacgtgacgg cggtatggtc gcaaccgtta tgccgcacgg cgtcttgttt agagggggtg
     1261 aggaaaaagc catccgtgcc ggtatcgtcg aagacgattt actggaagca gttctgggtg
     1321 ttgcacccaa cttgttttat ggcaccggga tacccgcatg catccttgtt cttcgtcaac
     1381 gtgtacaaaa tggtgccaac cgtgttagtg gcaagccagc agaacgtcgg ggcaaggtgt
     1441 tgtttattaa cgccgatcgc gagtttttcg aaggtcgcgc tcaaaaccac ctgcttccgg
     1501 aacatattga gaaaatcgtg acgactttta atgaatttaa acaagttgaa ggattcagcg
     1561 ccatcgttga taacgctacc ttaaaagcta atgattataa tctgaatatt cgccgctacg
     1621 cagataacac tccgcccccg gagccgcatg acgtccgtgc ccatctggtg ggcggggtcc
     1681 cgaaagcgga agtccaggcg aaatctactc tgtttgccgc gcacggtctg aatcccatgg
     1741 atctgttcgt tgaacgtgat gcgaaatacg tggattttaa gccggaatta gcgaaacgcc
     1801 gtgacctgaa accaagtatt gaaagaaatg caggtctggt tgctaaagag gccgcgatac
     1861 gcgctgcctt cgaacaatgg tggcaccagc acagcgcacg catcacctta ctggctggac
     1921 aaatggacaa cgccgcttcg ttggtgggtc tgcgcaatga tttattggcc tctttctccg
     1981 aaagcctgga ggccattgga atgctggatc cgtttcaggt acggggtatt gtagcaggct
     2041 tttggtatca aaccaaatac gacttcttaa ccctgatggc tcgcggcgcc aaaggtgtcg
     2101 ctgatgcctg gcgcacctcg atagtgacag cgctggaaga caaagccaat aaggagagcc
     2161 ctctggacca caagcttgta aagttcttaa tgtccgattt tgtggatgct ataaatgagt
     2221 tagaagcaaa acgtgctgaa ctggattcac agataaaggc cgcagaccca aaatcgggta
     2281 aggacgccga gggcgatgag ggggatgcgg ccgaatctag tgaagacgaa ccagctgatg
     2341 acaatgccgt tgacgaaacc caacttaagg cttggaaaaa agaattggca acagcgaaaa
     2401 aagctttaaa agcaaagaaa gactcatttg ctgcacacct taacagagaa gtcgatggtt
     2461 tgtctccgga gcaggccgca gaactgctgc ttactattct gcacaacgat atgcgtgcca
     2521 ttgtggagag atatattgcg gcgcagcgta aggcaatcgt tgttgccttt gaaaattggt
     2581 gggacaagta ccgtgttacc ttaaccgaga tagaagggaa acgcgattct gcggcgcaag
     2641 ccttacaagt ttttttaaag ggtcttcgtt acgtttgaat aggaggtgtt caatatgagt
     2701 aattttgtgg ccttgggtga tcatataaca gtgcagaagg gcaaagcgcc gctggttacg
     2761 gggtatgttg ggaaaggggc agagccttat cttagccctg aatatcttcg tggtagagca
     2821 ccggcagatc tggcgaaagc tggtccagac gcagttcgtg cagccgaggg cgaaactatt
     2881 ttgctgtggg acggtagtaa tgccggcgag ttttttcgga gcaaagtagg gctggtagct
     2941 tcaacgatga ccaaaatctc accaagctct gtgttccgcc cagcttattt ttttcatgtt
     3001 gcaaagcagg cggagagatt tctgaaggct caaacaaatg gaacaggcat cccccacgta
     3061 gatcgggagc ttttagaagg catcaaagta ttttgcccgg ggtccactga gcagcaactg
     3121 cttgcagaga ttttagatac tcttgacaca gctatatatg aaacagaagc aatcatagct
     3181 aaacttaaag cggtgaaaca gggtcttctg catgatttgt taactcgcgg tattgatgct
     3241 aacggagaac tgcgtccacc acaagccgaa gcgccgcatc tgtatgaatc ttcgccactg
     3301 ggctggattc cgaatgaatg gggtttagct ccaacggcta cacgctgcca cttgatcacc
     3361 aaaggaacaa cgccagctgc aaacgaaatg tggcagggtg gcgctggcat cagatttctg
     3421 agagtcgata acttatcctt tgacggacaa cttgatttgg atgcttctac atttagagta
     3481 agtctggcca cacataaagg cttccttgcc cggagtcggt gtctggaagg tgatgttctg
     3541 actaacattg tgggtccccc actgggcaaa ctgggcttag ttacgaaaga gatcggcgag
     3601 gtcaacatta accaggctat tgcgcttttc cgtccaactg aacagttgtt accaaaattc
     3661 ctgcttattt ggttgtcatc gtcaataagt caatcttggt tacgcaatcg tgcgaaacag
     3721 acgtcagggc aggtaaattt gaccttagcg ctttgtcaag aactgccgct tccccgtatg
     3781 accatcaatg agcagcaggc tatcgttgat cgggtggacg ccgctcagga gcagatctgg
     3841 tgtgaagaag agctgatacg caaaatgcgc cttgagaaaa gcggtctgat ggatgactta
     3901 ttaactgggc gcgtccgcgt taaaccgcag ttagcagaaa ctaagcaagc cgggagtgcg
     3961 tga
//
