LOCUS       New Construct      1418 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1418
                     /gene="M.Cac824I"
                     /codon_start=1
                     /translation="MHTHKFTVGSLFAGVGGICLGFKTAEYRGLGYNLIWANEIDEYA
                     GETYRHNFDHNLIAGDIEKIVDLNIIQSEQNRYINIMNEVATDEERIKYKVLIEKCEE
                     EKQIYREKQKQILSSKIDVLNGGFPCQAFSIAGEQKGFDDHRGNLFISIIKLIRLLDS
                     VHGKPRVLFLENVKNLMSHDGGRTYKVIKSKLEKEGYIIKEKVLNTMDFSHLPQNRER
                     IYIVGFLNKKDAEKFTMFDNIGEFYINKTVDERVRDIKKVIDYTLTKNTGDKYYYTKE
                     KYPKYFITEEEYDKIPESYRKDVRINLDESITEEYQFYQLRRGMYVRKNMSNVCPTLT
                     ANMGTGGHNVPLIKVKDGIRKLTPKETFKLQGFPVGNGYELPTKYINRPYPESRLYKQ
                     AGNAVSVPIIRLIAEQILRVLEE*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgcatactc
      181 acaaattcac cgttggttca ctgtttgctg gagttggcgg aatatgtttg ggattcaaaa
      241 cagccgaata taggggtctt ggatataacc ttatttgggc aaacgaaata gatgaatacg
      301 caggtgaaac atacagacat aattttgacc ataatctgat tgctggagac atagaaaaga
      361 ttgtggatct gaatataatt cagtcagagc agaataggta tataaacata atgaatgaag
      421 tagcaactga cgaagaaaga attaaatata aagttcttat tgaaaagtgc gaggaggaaa
      481 aacagatata cagagaaaaa cagaaacaga tacttagctc taagattgac gttttgaatg
      541 gaggctttcc gtgccaagca ttttccattg caggagaaca aaaaggattc gacgatcata
      601 ggggaaacct gtttatttct attattaaat taattaggtt gttagacagc gtacatggta
      661 aacccagagt gttgtttttg gaaaatgtta aaaatcttat gagccatgat ggtggaagga
      721 catataaagt tataaaatcc aaactggaaa aagaaggcta tattataaag gaaaaagtac
      781 ttaacaccat ggactttagc catcttcccc agaatagaga aaggatatat atagtgggat
      841 ttctgaataa gaaagatgcg gaaaagttca ccatgtttga taatataggc gaattttaca
      901 ttaacaagac agttgacgaa agggtaagag atattaaaaa agtaattgat tatacgctta
      961 ctaaaaatac aggagacaag tattattaca caaaagagaa gtatccgaaa tattttataa
     1021 cggaggaaga atacgataag ataccggagt catacaggaa agatgttaga attaatttgg
     1081 acgagagcat aacggaagaa taccagtttt atcagctgag aagaggaatg tatgtgagaa
     1141 agaatatgag caatgtttgt cccacattaa cagcgaatat gggcactgga ggacataatg
     1201 ttccgctgat aaaggtgaag gatggcataa gaaaactgac ccctaaggag acatttaaac
     1261 tgcagggctt tcccgtaggc aatggctatg agctgccgac aaagtatata aacagacctt
     1321 acccagaatc cagattgtat aaacaggctg gaaatgctgt gtccgtaccc ataattaggt
     1381 tgatagctga acaaattctt agagtgcttg aagagtga
//
