LOCUS       New Construct      3165 bp    DNA         SYN       20-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1619
                     /gene="M.Mel0051"
                     /codon_start=1
                     /translation="MTNQEIVQRLWNECNVLRDDGITYQDYVTELTYILFLKMSKEQG
                     QEEGIPERYRWDELKAKEGLELKTFYKQMLLDLGNAQKTPNPKVNAIYKDASTSIDEP
                     ANLEKIIKDIDELDWFSAREEGLGNLYEGLLEKNANETKSGAGQYFTPRPLINMMVRM
                     VHPKPGERLCDPAAGTFGFMVAANDYLKKQTDDYFDLSDEEQQFQRHEAFSGMELVPT
                     THRLALMNEYLHDMDGRLEQGDSLSAAGKWMKDFDVILTNPPFGTKKGGERATRDDLT
                     YATSNKQLNFLEIIYNALNRKGTARAAVVVPDNVLFADGAGEQIRRDLMNKCNLHTIL
                     RLPSGIFYAQGVQTNVLFFDRGTTDQNNTAEIWIYDMRHKMRTFGKRSPLNERDFAEF
                     EKLYCPDNRTERKETYDAKNNTEGRWRKFTKADIEARPNTSLDISWMTDEEEADTRSL
                     SEILTAMQEKSEEIAQAVALLNQELAGVDQDD*"
     misc_feature    1620..1635
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1636..3165
                     /gene="S.Mel0050"
                     /codon_start=1
                     /translation="MTKNLHIDTQAMRAKILDLAIQGKLTDQREEDGNARDLLREIQA
                     EKEKLIKEKKIKKGKPLPEITEEEKPFEIPENWEWVRLGDIAQAVNGDRGKNYPKREE
                     YVASGIAWINAGHITDDGYLNADKMNYITPNKYDSLRNGKIQRNDLLFCLRGSFGKVA
                     RIEPFCEGAIASSLAIIRLFNTALRAYLFIYLKSPQAANELNKYANGTAQPNLAAKDV
                     LKYLIPLPPLAEQHRIADKVSALFAQLDAINKAYEEYRELQQAMKAKLLDLAMQGKLT
                     DQRKEDGDARDLFNDIQKEKQRLIAEKKIKKEKPLPEIEADEIPFEIPTNWMWARLGD
                     IVSKTIKRGKSPKYSITGDVLVFAQKCNTKPGRINLELAKKLDLSTLKKYPEEEFMLS
                     GDIVINSTGNGTMGRVGFFRDKDNPYNLKIVPDSHVTTIRVLHDLIDSQYIFYFLKKL
                     QPKLESSGDGSTNQKELKATIIASICIPLPPLDEQKRIVAKLDELLPLCNVPGVYSKG
                     K*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgacaaacc
      181 aggaaatcgt gcagcggctg tggaatgaat gcaacgttct gcgcgacgac ggtatcacgt
      241 atcaagacta tgtgactgaa ttaacatata ttttatttct gaaaatgtca aaggagcaag
      301 gccaagagga aggcatcccg gaacgttatc ggtgggatga actgaaagcg aaggaaggtc
      361 ttgaacttaa aacattctat aagcagatgt tgctggatct tggcaatgca cagaaaactc
      421 cgaatccaaa agttaatgcc atatacaaag atgcatcaac atccatcgat gagcctgcga
      481 atttggagaa aattattaaa gacattgatg agcttgattg gttcagcgcc cgcgaagagg
      541 gtctgggaaa cctgtacgaa gggctgcttg agaaaaatgc aaatgaaact aaaagcggtg
      601 caggccaata cttcacacca cgcccactta ttaatatgat ggtgcgtatg gttcacccta
      661 agccgggtga gcgtttatgc gatcctgccg ctggaacctt tggctttatg gtagcggcaa
      721 atgactacct gaaaaagcaa acggatgatt atttcgacct tagcgatgag gaacagcagt
      781 ttcagagaca tgaagcattt tcgggaatgg aattggtacc gacaactcac agattagcgt
      841 taatgaacga gtatctgcat gatatggacg gtcgcttaga acagggtgac tcactgtctg
      901 cggccggtaa atggatgaag gattttgatg ttattttgac caaccctcca tttgggacca
      961 agaagggggg cgagcgcgct acacgcgacg atctgacata tgcaacaagc aacaagcagt
     1021 taaactttct ggagataata tacaatgctt taaatcgtaa aggaactgcc cgcgcagccg
     1081 tcgtagtgcc ggataatgta cttttcgcgg atggagcagg agagcagata cgtcgtgatt
     1141 tgatgaataa gtgtaatctt catacaattc tgagattgcc ctctggaatc ttttatgccc
     1201 agggtgtcca aaccaacgtg ctgttcttcg accgcggcac tacagaccag aataatactg
     1261 ctgaaatttg gatttatgat atgcgtcata aaatgcgtac gtttggcaag cgctctcccc
     1321 ttaatgaacg tgactttgct gagtttgaaa aactttattg tccagataat cgtaccgagc
     1381 ggaaagagac ctatgatgct aaaaataaca ccgaaggtcg ctggcgtaag tttactaaag
     1441 ctgatatcga agcgcgccca aatacgagtc tggatatatc ctggatgaca gatgaagaag
     1501 aagccgacac tcgctccctg tcagagatac tgaccgcgat gcaggagaag tccgaggaaa
     1561 tagcgcaagc ggtagccctg ctgaatcagg agcttgccgg cgttgatcaa gatgactgaa
     1621 taggaggtgt tcaatatgac gaaaaatctg catatcgaca cccaagccat gcgggcaaaa
     1681 atactggacc tggcaattca gggcaagctg acggatcagc gtgaagaaga tggaaacgca
     1741 cgcgacctgc tgcgcgagat tcaagccgaa aaggagaaat tgatcaaaga aaaaaaaata
     1801 aagaaaggta agccgttgcc agagattaca gaagaagaaa agcctttcga aatacctgag
     1861 aattgggagt gggtgcggct gggagacata gcccaagctg ttaacggtga tcgcgggaaa
     1921 aactacccga aacgcgaaga gtatgtggcc agcggcatcg cttggattaa cgcgggtcat
     1981 attaccgatg atggctacct gaatgcagat aagatgaact acattacgcc gaacaagtat
     2041 gactccctgc gcaatggcaa aattcagcgt aatgacttgc tgttttgtct gagaggaagc
     2101 ttcggcaaag tagcccgtat tgagccgttt tgtgaggggg ctatagcatc ttccttagcg
     2161 atcatacgcc tgtttaacac cgcgctgcgt gcctatctgt tcatatacct gaaatcccct
     2221 caagcggcca acgaattgaa caaatacgct aatgggacgg cgcagcctaa tttagccgcg
     2281 aaggatgttc ttaaatactt aatcccgctt cctccgttag ctgagcagca ccggattgct
     2341 gataaagtaa gcgcactttt cgcgcagctt gatgccatca ataaagcata tgaagaatac
     2401 cgcgaattac agcaggccat gaaagccaag ctgcttgatc ttgctatgca gggtaaactg
     2461 acagaccaaa gaaaggaaga tggcgatgca cgtgacttat tcaacgatat tcagaaagag
     2521 aagcagcgct tgattgccga gaagaaaatt aaaaaggaaa aacctctgcc tgaaatagaa
     2581 gctgatgaga tcccttttga gatcccgaca aattggatgt gggcccgtct gggcgacata
     2641 gtgagcaaga ccattaaacg tggaaaatct ccaaagtatt cgataactgg cgatgttctg
     2701 gtatttgcgc aaaagtgcaa cacaaagcct ggtcgcatta atctggagct ggcgaaaaaa
     2761 ctggatttgt cgaccctgaa aaagtaccca gaggaagaat tcatgctgtc gggggatatt
     2821 gtgataaata gcaccgggaa cgggactatg ggccgcgtgg ggtttttccg cgacaaagac
     2881 aatccgtata atctgaaaat cgtgccggat tcacatgtta caacaatccg tgttctgcat
     2941 gatcttattg acagtcagta tattttctat tttctgaaaa aactgcagcc aaaattagaa
     3001 agttcagggg atgggtccac aaatcagaaa gaattaaaag caactatcat agcttcaata
     3061 tgtattccac tgcccccgct tgatgagcag aaacgtatcg tcgccaaact ggacgaactg
     3121 ctgccactgt gtaatgtgcc gggggtttat tcgaaaggca agtga
//
