LOCUS       New Construct      3066 bp    DNA         SYN       5-4-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Synthetic"
     misc_feature    1..170
                     /product="T5P25lac-full+RBS"
                     /label="T5P25lac-full+RBS"
     CDS             171..1721
                     /gene="Moth_1672M"
                     /codon_start=1
                     /translation="MTENTNMDLSTLENWLWEAACVIRGAVDAPKYKDYILPLIFLKR
                     LSDVFEDEIARLAEEIFDSIEEALKQVEEDHALVRFYIPPQARWDAISRQTTNIGEYL
                     TSAVRAVARENPKLHGIFENIDFNAQMAGQPVIDNDRLYNLIQVLSRHRLGLKDVEVD
                     ILGRAYEYLLRKFAEGQGQSAGEFYTPREVTWLMAYLLEPRPGDEIYDPACGSGGLLI
                     KSVLALKETYGDDPRIAPVKIYGQEILYTTFAMAKMNAFIHDLEADIRLGDTMARPAF
                     TNPDGSLRTFDKVTANPMWNQKFPLPLYEEDPFDRFKFGGIPPASSADWGWIQHMFAS
                     LKEGGKMAVVLDTGSVSRGSGNQGSNRERDIRKVFVENDLVECVILLPENMFYNTTAP
                     GIIMVINKAKKHPAEILLINASKLFTKGRPKNYMEDEHIKQVYSIYREWREEEGLSKI
                     IPVEEAARNDYNLSPSRYVSINGKEEYRPIEEILVELAEVEEERQAVDKELNDILGKL
                     GFGGWLNG*"
     misc_feature    1722..1737
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             1738..3066
                     /gene="Moth_1671S"
                     /codon_start=1
                     /translation="MGKEVNEVKEGYKETEIGVLPEDWEVVRLGKVFEEVDRRVNNVK
                     NAASLPVLSLTKNNGIIPQTERFKKRIATDDLSNYKVVYKKELVYNPYVIWEGAIHIL
                     NRLEAGLVSPVYPVLSVNKKVADAYFFDFWLRTPSAIKAYSRYASGAVNRRRAIRKTD
                     FKNIDAPLPPLHEQRKIAYVLSTIQRAIQLQDKVIAATRELKKSLMRHLFTYGPVPVD
                     QIDRVPLKETEIGMVPEHWEVVRLREVADFTKKPRGLNYSGNIPFIPMELIPIGRVNI
                     QKYIIKPSSEISSGVYCEQGDLLLAKITPSFENYKQGIISQIPKPFAFATTEVYPIKA
                     RKDFLEILYLFYYLLIPQVRQDIAGKMEGTTGRQRISKSVIQNYLIPIPPLSEQRQIA
                     RFLITVDKKIEAEEYRKSTLQSLFQTMLHLLMTGKVRVKDLEVKEDALRQ*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgaccgaaa
      181 ataccaacat ggacctgtcg acattagaaa attggttatg ggaggcagct tgcgtcatcc
      241 gtggtgcagt tgacgcacca aaatacaagg attatattct tccattgatt tttttaaagc
      301 gcttaagcga tgtttttgag gatgagattg caagacttgc tgaggagata tttgattcaa
      361 tagaagaggc gttgaagcag gtggaagaag accatgcgct ggtgcgtttt tatattccgc
      421 cacaagcgcg ctgggatgcc atcagtcgcc agacgacaaa cattggtgaa tatttaacat
      481 cggctgttcg cgcggttgca agagagaatc cgaaattaca tggcatattt gagaatatag
      541 attttaatgc tcaaatggct ggccaacccg tcatagataa tgatcgttta tataatctta
      601 tacaggtgct gtctcgtcac cgtctgggct taaaagatgt ggaggtggat atcttaggac
      661 gtgcatatga atatctgtta cgcaaattcg cagagggtca ggggcagtca gcgggtgaat
      721 tttacacccc aagagaagtc acctggctta tggcctacct gttggaacct cggcctggcg
      781 acgagattta cgatcctgca tgtggtagcg gcgggctttt gattaaaagt gtgctggctc
      841 tgaaggagac ttacggtgat gatccacgca ttgcaccggt caaaatctac ggtcaggaaa
      901 ttttgtacac aacatttgcg atggccaaaa tgaatgcctt catacatgac cttgaggctg
      961 atatacgtct gggggatact atggctcgtc ctgccttcac aaatcccgat gggtctctgc
     1021 gtactttcga caaagtaacg gccaacccga tgtggaatca gaaatttccc ctgccgcttt
     1081 atgaggagga tcccttcgac cgttttaaat tcgggggtat ccctccggcg tcttcagcgg
     1141 actggggctg gatacaacac atgtttgctt cgctgaagga aggtggaaaa atggctgtgg
     1201 ttttagacac aggatcagta agccggggct ctgggaatca gggaagcaac agagaacgcg
     1261 acatccgtaa agttttcgtc gaaaacgatc ttgtcgaatg cgttatcttg ttgcctgaga
     1321 acatgtttta taacacaact gcgcctggca tcatcatggt cattaataaa gccaaaaaac
     1381 atcccgccga gatattgtta attaacgcgt ctaaactgtt cacaaaaggt cgcccgaaaa
     1441 attatatgga agatgagcat atcaaacagg tgtacagcat ttatcgcgaa tggcgggaag
     1501 aagaaggttt atcaaaaatc attcctgtgg aggaggctgc tagaaacgat tacaatctga
     1561 gccctagtcg ttatgtgtcg ataaacggga aggaagaata ccgcccgatt gaagaaattt
     1621 tagttgaact ggccgaagta gaagaagagc gccaagctgt cgataaagag ctgaacgata
     1681 ttcttggaaa attaggattt ggtgggtggc tgaatgggta aataggaggt gttcaatatg
     1741 ggcaaagaag ttaacgaggt taaagagggt tataaagaaa ctgagatcgg tgtgctgccg
     1801 gaagactggg aggttgttcg cctggggaaa gtcttcgaag aagtggatcg tagagtgaat
     1861 aatgtaaaga atgcggcctc cttaccggtg ttgtccctga ccaaaaacaa cgggattatt
     1921 ccgcagactg aaagatttaa aaagcgcatt gcaacggatg acttaagtaa ctataaagta
     1981 gtttacaaaa aagagttggt atacaaccct tatgtcattt gggaaggcgc tatccatatt
     2041 ctgaatcgtt tagaagccgg actggtgtca ccagtttatc cggttctttc agtaaacaag
     2101 aaggtcgcgg atgcttattt tttcgatttc tggttacgga ctccctccgc tataaaagcg
     2161 tatagccgtt atgcttcagg tgccgttaat agacggcgtg ccattcgcaa gactgatttt
     2221 aaaaacatag atgcaccgtt acccccactg catgagcagc ggaaaattgc ttatgttttg
     2281 tcaaccattc agcgcgcgat acagctgcag gataaagtta ttgcggctac gagagagttg
     2341 aagaaatcat tgatgcgtca cttatttacg tacggccccg tcccagtcga ccaaattgat
     2401 cgggttcctc tgaaggaaac cgaaattggc atggtaccgg aacattggga agtagtccgt
     2461 ttgcgcgaag tagcggattt tactaaaaaa ccccgcgggc tgaattatag cggtaatata
     2521 ccttttatac cgatggagct tattccaatt ggtcgtgtta atatccaaaa atacattatt
     2581 aaaccgagta gcgaaatctc tagtggcgtg tattgcgagc aaggagattt gttgttggcg
     2641 aaaattactc ctagtttcga gaactataaa caaggtatta tcagtcagat cccaaaaccg
     2701 tttgcgtttg cgacaactga agtttatccg ataaaggctc gcaaagattt ccttgaaatt
     2761 ctttaccttt tttactatct gctgattccg caggttcgcc aggacattgc aggaaaaatg
     2821 gagggaacca cgggtcgcca gcgtattagc aaaagtgtta tacaaaacta tctgatccct
     2881 attcccccgc tgagtgaaca acgccagatt gcgagatttt taataactgt agacaaaaaa
     2941 atcgaagcgg aagagtatag aaaaagcaca ctgcaaagcc tgttccaaac catgctgcac
     3001 ttactgatga cagggaaagt ccgtgtgaaa gatctggagg ttaaggaaga cgcgctgcgc
     3061 caataa
//
