LOCUS       New Construct      1073 bp    DNA         SYN       19-3-2014
ACCESSION   New Construct
KEYWORDS    
SOURCE      Synthetic
  ORGANISM  Synthetic
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Synthetic"
     misc_feature    1..154
                     /product="T5P25lac-full-no RBS"
                     /label="T5P25lac-full-no RBS"
     misc_feature    155..170
                     /product="RBS+7"
                     /label="RBS+7"
     CDS             171..1073
                     /gene="M.MseI-TTAA"
                     /codon_start=1
                     /translation="MPISTVWTPDGDDLIVEADNLDFIQTLPDASFRMIYIDPPFNTG
                     RTQRLQSLKTTRSVTGSRVGFKGQTYDTVKSTLHSYDDAFTDYWSFLEPRLLEAWRLL
                     TPDGALYLHLDYREVHYAKVVLDAMFGRESFLNELIWAYDYGARSKSKWPTKHDNILV
                     YVKDPNNYVWNGQDVDREPYMAPGLVTPEKVALGKLPTDVWWHTIVPPASKERTGYAT
                     QKPVGIIRRMIQASSNEGDWVLDFFAGSGTTGAAARQLGRRFVLVDVNPEAIAVMAKR
                     LDDGALDTSVTIVQTPQSDPRTDG*"
ORIGIN
        1 aattgtgagc ggataacaat tacgagcttc atgcacagtg aaatcatgaa aaatttattt
       61 gctttgtgag cggataacaa ttataatatg tggaattgtg agcgctcaca attccacaac
      121 ggtttccctc tagaaataat tttgtttaac ttttatagga ggtgttcaat atgccaatta
      181 gcacagtgtg gacgcccgac ggagacgact taatagttga ggccgataat cttgatttta
      241 ttcaaacatt accagatgca agctttagaa tgatttatat tgaccccccg tttaatacag
      301 gaagaacaca aaggttgcag agtttaaaga caactagaag tgtaacaggt agtagagtag
      361 gctttaaagg acaaacctac gacactgtta agtcaacttt acattcctat gatgatgcgt
      421 ttacagacta ctggtcattt cttgaaccga ggctgctgga agcttggagg cttttaacac
      481 cggatggtgc tctttacttg catttggact acagagaagt tcattatgct aaagtagtgc
      541 tggacgctat gtttggaagg gagtcgttcc tgaacgaact tatttgggcc tacgattatg
      601 gagccagatc caaaagtaaa tggccgacta aacatgataa tatacttgta tatgtgaaag
      661 acccgaataa ctatgtgtgg aacggacaag acgtagacag agagccgtac atggcaccgg
      721 gattagtgac tcctgaaaaa gttgcgttag gcaaacttcc gacagacgtt tggtggcata
      781 ctatagtacc gccagcgtca aaagaaagaa ccggttatgc gacgcagaaa cctgtaggaa
      841 taattaggag aatgatacag gcaagttcaa acgagggcga ttgggtactt gatttttttg
      901 caggctctgg cactactgga gctgctgcta gacaattagg taggaggttt gttctggtag
      961 atgttaatcc cgaagccata gcagtaatgg ccaagagatt agacgacgga gcattggata
     1021 ctagtgtgac aattgttcag actcctcagt ctgaccccag aacagacgga taa
//
